Co-Chemotherapy Effect of Glycosylated Nanoalbumin Genitri Seed Extract Targeting Induced Apoptotic on Overexpressed HER2+ Breast Cancer
Abstract
HER2-targeted therapy aims to stop proliferation and induced apoptosis. Genitri seeds show anticancer effects and have potential as co-chemotherapy. To maximize bioactive delivery, a glycosylated-nanoalbumin delivery system is used. This research aims to explore the ability of Glycosylated-Nanoalbumin Genitri Seeds (GN-GSE) to induce apoptosis in MCF-7/HER2 cancer cells. The cytotoxic test using the MTT assay showed GSE selectivity (SI=2.7) against cancer cells (IC50 104 μg/mL) and non-toxicity against normal cells (IC50 284 μg/mL). Induction of apoptosis occurs through inhibition of the CDK1 protein which is predicted by molecular docking. GSE has the potential as a synergistic co-chemotherapy with tamoxifen (CI<1) and has good affinity for the CDK1 inhibitory domain. Elaeocarpenine is known to have good affinity with a greater ΔG value (-11.40 kcal/mol) than the native ligand. The GN-GSE formula meets the criteria for nanoparticles with good stability. This research shows that GSE has anti-cancer activity, making it potential as a therapy for HER2 overexpressed breast cancer as well as in a glycosylated nanoalbumin drug delivery system.
Keywords: Apoptotic induced, co-chemotherapy, genitri seeds, glycosylated, nanoalbumin.
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DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev15iss3pp198-209
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