Antioxidant Activity and Cytotoxic Potential of Parijoto (Medinilla speciosa (Reinw ex BL)) Fruit Fractions on HeLa Cell Line

Aji Winanta, Linta Sabila Hanik, Rifki Febriansah

Abstract


Parijoto (Medinilla speciosa (Reinw ex BL)) is one of the Indonesian indigenous plants widely used as traditional medicine. A previous study showed that ethanol and methanol extracts of Parijoto fruit could inhibit T47D breast cancer cells. This study explores the antioxidant and cytotoxic activities of Parijoto fruit extract and fractions on HeLa cell line. The fruits were extracted using ethanol 70% and fractionated by hexane and ethyl acetate. Furthermore, the fraction was analyzed for secondary phytochemical metabolite content using thin-layer chromatography and staining reagents. The antioxidant and cytotoxic activities were determined using the DPPH scavenging assay and the MTT assay, respectively. The ethanol extract and fraction contained flavonoid and tannin compounds. Ethanol extract, ethanol fraction, and ethyl acetate fraction of Parijoto fruit had an antioxidant activity with IC50 values of 77.3, 88.64, and 46.61 μg/mL, respectively. Ethyl acetate fraction showed the highest activity on HeLa cells with an IC50 value of 45.57 μg/mL compared to ethanol extract, ethanol fraction and n-hexane fraction with an IC50 value of 233.43, 700.75, and 534.30 μg/mL, respectively. Based on these results, the ethyl acetate fraction of Parijoto fruit had the potency to be explored further to elucidate their cytotoxicity mechanism in HeLa cells.


Keywords: antioxidant activity, cytotoxicity, Medinilla speciosa, Parijoto fruit fractions.


Full Text:

PDF

References


Abotaleb, M., Samuel, S., Varghese, E., Varghese, S., Kubatka, P., Liskova, A. and Büsselberg, D., 2018, Flavonoids in Cancer and Apoptosis, Cancers., 11, 28. CrossRef

Amić, D., Davidović-Amić, D., Bešlo, D. and Trinajstić, N., 2003, Structure-radical scavenging activity relationships of flavonoids, Croat. Chem. Acta., 76(1), 55–61. Link

Ayoola, G., Coker, H., Adesegun, S., Adepoju-Bello, A., Obaweya, K., Ezennia, E. and Atangbayila, T., 2008, Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria, Trop. J. Pharm. Res., 7(3), 1019–1024. CrossRef

Buitrago-Pérez, Á., Garaulet, G., Vázquez-Carballo, A., Paramio, J.M. and García-Escudero, R., 2009, Molecular Signature of HPV-Induced Carcinogenesis: pRb, p53 and Gene Expression Profiling, Current Genomics., 10, 26–34. CrossRef

Chahar, M., Sharma, N., Dobhal, M. and Joshi, Y., 2011, Flavonoids: A versatile source of anticancer drugs, Pharmacogn., 5(9), 1-12. CrossRef

Es-Safi, N.-E., Ghidouche, S. and Ducrot, P., 2007, Flavonoids: Hemisynthesis, Reactivity, Characterization and Free Radical Scavenging Activity, Molecules., 12, 2228–2258. CrossRef

Harborne, T.B., 1987, Metode Fitokimia: Penuntun Cara Moderen Menganalisis Tumbuhan. ITB Bandung.

Huang, C.-Y., Ju, D.-T., Chang, C.-F., Muralidhar Reddy, P. and Velmurugan, B.K., 2017. A review on the effects of current chemotherapy drugs and natural agents in treating non–small cell lung cancer, BioMedicine., 7(4), 12-23. CrossRef

Husnaa, U., Laksmiani, N.P.L., Susidarti, R.A. and Meiyanto, E., 2017, Ethyl Acetate Fraction of Caesalpinia sappan L. Enhances Cisplatin’s Cytotoxicity on HeLa Cells via G1 and S Arrest through p53 Expression, Indones, J. Cancer Chemoprevention., 8(2), 51-56. CrossRef

Jork, H. (Ed.), 1990, Thin-layer chromatography: reagents and detection methods. Vol. 1 a: Physical and chemical detection methods Fundamentals, reagents I. VCH, Weinheim. Link

Kaulika, N. and Febriansah, R., 2019, Chemopreventive Activity of Roselle’s Hexane Fraction against Breast Cancer by In-Vitro and In-Silico Study, Proceedings, Yogyakarta,: The Third International Conference on Sustainable Innovation 2019 – Health Science and Nursing (IcoSIHSN 2019), Atlantis Press: Advances in Health Sciences Research., 15, 66-71. CrossRef

Krueger, R.J., 2005, Drugs of Natural Origin, J. Nat. Prod., 68, 629–629.

Man, S., Gao, W., Zhang, Y., Huang, L. and Liu, C., 2010, Chemical study and medical application of saponins as anti-cancer agents, Fitoterapia, 81, 703–714. CrossRef

Manuaba, T.W., 2010, Panduan penatalaksanaan kanker solid PERABOI 2010, Sagung Seto: Jakarta.

Mardawati, E., Filianty, F., Marta, H., 2008, Kajian Aktivitas Antioksidan Ekstrak Kulit Manggis (Garcinia mangostana L ) Dalam Rangka Pemanfaatan Limbah Kulit Manggis di Kecamatan Puspahiang Kabupaten Tasikmalaya, TEKNOTAN: Jurnal Industri Teknologi Pertanian, 2. CrossRef

Meenakshi, S., Umayaparvathi, S., Arumugam, M., Balasubramanian, T., 2011, In vitro antioxidant properties and FTIR analysis of two seaweeds of Gulf of Mannar, Asian Pac. J. Trop. Biomed., 1, S66–S70. CrossRef

Ministry of Health RI, 2006, Inventaris Tanaman Obat Indonesia (V), Balai Penelitian Tanaman Obat, Jakarta.

Octaviani, I., 2016, Aktivitas Antibakteri Ekstrak Daun Parijoto (Medinilla speciosa) Terhadap Escherichia coli dan Staphylococcus aureus, Thesis, Universitas Atma Jaya Yogyakarta, Yogyakarta. Link

Oun, R., Moussa, Y.E. and Wheate, N.J., 2018, Correction: The side effects of platinum-based chemotherapy drugs: a review for chemists, Dalton Trans., 47, 7848–7848. CrossRef

Phang, C.-W., Malek, S.N.A., Ibrahim, H., 2013, Antioxidant potential, cytotoxic activity and total phenolic content of Alpinia pahangensis rhizomes, BMC Complement. Altern. Med., 13, 243. CrossRef

Ren, W., Qiao, Z., Wang, H., Zhu, L. and Zhang, L., 2003, Flavonoids: Promising anticancer agents, Med. Res. Rev., 23, 519–534. CrossRef

Riss, T.L., Moravec, R.A., Niles, A.L., Duellman, S., Benink, H.A., Worzella, T.J. and Minor, L., 2013, Cell Viability Assays, in: The Assay Guidance Manual. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences, pp. 1–25.

Tiwari, P., Kumar, B., Kaur, M., Kaur, G. and Kaur, H., 2011, Phytochemical screening and Extraction: A Review, Internationale Pharmaceutica Sciencia, 1, 98–106. Link

Tusanti, I., Johan, A. and Kisdjamiatun, R., 2014, Sitotoksisitas in vitro ekstrak etanolik buah parijoto (Medinilla speciosa, reinw.ex bl.) terhadap sel kanker payudara T47D, J. Gizi Indones. Indones. J. Nutr., 2, 53–58. CrossRef

Vifta, R.L. and Advistasari, Y.D., 2018, Skrining Fitokimia, Karakterisasi, dan Penentuan Kadar Flavonoid Total Ekstrak dan Fraksi-Fraksi Buah Parijoto (Medinilla speciosa B.), Proceeding, Semarang: Seminar Nasional , pp: 8-13. Link

Weerapreeyakul, N., Nonpunya, A., Barusrux, S., Thitimetharoch, T. and Sripanidkulchai, B., 2012, Evaluation of the anticancer potential of six herbs against a hepatoma cell line, Chin. Med., 7, 1-7. CrossRef

World Health Organization, 2018, Cancer., Website, https://www.who.int/news-room/fact-sheets/detail/cancer, accesed on May 24, 2019.

Yang, L.-L., Lee, C.-Y. and Yen, K.-Y., 2000, Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells, Cancer Lett., 157, 65–75. CrossRef

Yang, X.-W., Dai, Z., Wang, B., Liu, Y.-P., Zhao, X.-D. and Luo, X.-D., 2018, Antitumor Triterpenoid Saponin from the Fruits of Avicennia marina, Nat. Prod. Bioprospecting., 8, 347–353. CrossRef

Zhang, J., Chen, D., Han, D., Cheng, Y., Dai, C., Wu, X., Che, F. and Heng, X., 2018, Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells, Oncol. Lett., 15, 6845-6850. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev12iss2pp74-82

Copyright (c) 2021 Aji Winanta

Indexed by:

               

               

      

 

Indonesian Society for Cancer Chemoprevention