Estrogenic Activity of Mahoni Seed Ethanolic Extract [Swietenia mahogany (L.) Jacq] on Uterus Weight, Bone Density and Mamae Gland Proliferation on Ovariectomized Rats

Poppy Anjelisa Zaitun Hasibuan, Rosa Gloria Sitanggang, Robbani Syahfitri Angkat

Abstract


Menopause is a hypoestrogenic condition due to decreased function of the ovary. During menopause there is no reserved ovum in the ovary, as a result the synthesis of estrogen by the follicles does not take place. Deficiency of estrogen can lead to discomfort and decrease in the women quality of life. Therefore, supplements from natural resources to reduce menopausal symptoms will be needed. The objectives of the study were to determine the effect of mahogany seeds ethanolic extract (MSEE) on the development of uterus, bone density, and mammae gland proliferation on ovariectomized rats. Extract was made by maceration using 96% ethanol as the solvent, then the study of estrogenic effect was carried out on 30 female rats which were divided into 6 groups. Group 1 (normal control), group 2 (positive control) given estradiol dose of 0.18 mg/kg body weight (BW), group 3 (negative control) given Na-CMC 1% and group 4, 5, 6 given MSEE orally for 14 consecutive days with doses of 50, 100, 200 mg/kg BW. Data were analysed using ANOVA then continued with Tukey HSD Post Hoc test to see the differences between the treatments. The results of the study showed that MSEE was able to increase the weight of the uterus, the length of estrus phase in the estrus cycle, bone density and the mammae gland proliferation of rats. The results concluded that MSEE has phytoestrogenic effect on ovariectomized rats.


Keywords: phytoestrogen, ovariectomy, uterus weight, bone density, mammae proliferation


Full Text:

PDF

References


Agustini, K., Wiryowidagdo, S. and Kusmana, D., 2005, Pengaruh Pemberian Ekstrak Biji Klebet (Trigonella foenum-graecum L.) Terhadap Kadar Hormon Estradiol Dan FSH Plasma Tikus Putih Betina Galur Wistar Prepubertal, Majalah Ilmu Kefarmasian, 2(2), 74-83. CrossRef

Anderson, E., Clarke, R.B. and Howeli, A., 1998, Estrogen Responsiveness and Control of Normal Human Breast Proliferation, Journal of mammary Gland Biology and Neoplasia, 3(1), 23-35. CrossRef

Aswar, U., Gurav, M., More, G., Rashed, K. and Aswar, M., 2014, Effect of aqueous extract of Solanum xanthocarpum schard. & Wend. on postmenopausal syndrome in ovariectomized rats, Journal of integrative medicine, 12,(5), 439-446. CrossRef

Chlebowski, R.T., Kuller, L.H., Prentice, R.L., Stefanick, M.L., Manson, J.E., Gass, M., et al., 2009, Breast Cancer after Use of Estrogen plus Progestin in Postmenopausal Women, N. ENGl. J. Med., 360(6), 573-587. CrossRef

Dianingati, R.S., Novarina, A., Hana, A.K., Muntafi’ah, L. and Lukitaningsih, E., 2015, The combination of High Calcium Milk With Citrus Maxima Peels Ethanolic Extract Increased Bone Density of Ovariectomized Rats, Indonesia J. Cancer Chemoprevent, 6(2), 42-48. CrossRef

Gerard, C., Blacher, S., Communal, L., Courtin, A., Tskitishvili, E., Mestdagt, M., et al., 2015, Estetrol is a week estrogen antagonizing estradiol-dependent mammary gland proliferation, Journal of Endocrinology, 224(1), 85-95. CrossRef

Jie, D., Ying, X., Xiaoping, M., Jinna, A., Xiudong, Y., Zhiqiang, L., et al., 2015, Estrogenic effect of the extract of Ranshen (Radix ginseing) on reproductive tisssue in immature mice, Journal of traditional Chinese Medicine, 35(4), 460-467. CrossRef

Keiler, A.M., Dorfelt, P., Chatterjee, N., Helle, J., Bader, M.I., Vollmer, G., et al., 2015. Assessment of the effects of naringenin-type flavones in uterus and vagina, Journal of steroid Biochemistry and molecular biology, 145(1), 49-57. CrossRef

Keiler, A.M., Macejova, D., Dietz, M.B., Bolton, J.L., Pauli, G.F., Chen, S.N., et al., 2017, Evaluation of estrogenic potency of standardized hops extract on mammary gland biology on MNU- induced mammary tumor growth in rats, Journal of steroid Biochemistry and molecular biology, 174(1), 234-241. CrossRef

Lestari, B., Walidah, Z., Utomo, R.Y., Murwati, R. and Meiyanto, E., 2019, Suplementation with extract of pumpkin seeds exerts estrogenic effects upon the uterin, serum lipids, mammary glands, and bone density in ovariectomized rats, Phytotherapy research, 2019, 1-10. CrossRef

Liu, H., Xiong, Y., Wang, H., Yang, L., Wang, C., Liu, X., et al., 2018, Effects of Water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model, Journal of Ethnopharmacology, 221(1), 126-136. CrossRef

Lusiana, N., 2017, Pengaruh Fitoestrogen Daging Buah Kurma Ruthab (Phoenix dactylifera L.) terhadap sinkronisasi siklus estrus mencit (Mus musculus L.,) betina, Klorofil, 1(1), 24-31. Link

Mendelsohn, M.E. and Karas, R.H., 2010, Rapid Progess for Non-nuclear Estrogen Receptor Signaling, J. Clin. Invest., 120(7), 2277-2279. CrossRef

Murkies, A.L., Wilcox, G. and Davis, S.R., 1998, Phytoestrogen, Journal of Clinical Endocrinology and Metabolism, 83(2), 297-303. CrossRef

Nurrochmad, A., Leviana, F., Wulancarsari, C.G. and Lukitaningsih, E., 2010, Phytoestrogen of osteoporosis, International Journal of Phytomedicine, (2), 363-372. Link

Novitasari, D., Devyanto, H.T., Arifah, F.H., Ivanawati, A., Ulum, Z. and Murwanti, R., 2018, Estrogenic Activity of Ethanolic Extract of Papaya Peels (Carica Papaya L.) on Uterine Weight And Mammae Gland Proliferation on Ovariectomy Rats, Indonesia J. Cancer Chemoprevent, 9(2), 86-91. CrossRef

Parhizkar, S., Latiff, L.A., Rahman, S.A., Dollah, M.A. and Parichehr, H., 2011, Assesing Mahogany (Swietenia Mahogani L. Jacq) Seeds In The Liver Tissue of Diabetic Experimental Rats Estrogenic Activity of Nigella sativa in Ovariectomized Rats Using Vaginal Cornification Assay, African Journal of Pharmacy and Pharmacology, 5(2), 137-142. CrossRef

Prasetiawan, E., Adnyane, K.M. and Wresdiyati, T., 2017, The Antioxidant Activieties of Ethanol Extract of Swietenia mahogany L., Jurnal Kedokteran Hewan, 11(2), 57-61. CrossRef

Ramos-Vara, J.A., 2005, Technical Aspects of Immunohistochemistry, Vet Pathol, 42, 405-426. CrossRef

Salahuddin, M.S., Safitri, E., Yunita, M.N., Susilowati, S., Hamid, I.S. and Yudhana A., Pengaruh Ekstrak Kedelai (Glycine max) Terhadap Proliferasi Lapisan Endometrium Mencit (Mus musculus), Jurnal Medik Veteriner, 2(1), 49-54. CrossRef

Sirotkin, A.V. and Harrath, A.H., 2014, Phytoestrogens and Their Effects, European Journal of Pharmacology, 741, 230-236. CrossRef

Triutomo, D.H., Miranda, A., Tamba, L.J. and Lukitaningsih, E., 2017, Estrogenic Effect Ethanol Extract Corn Silk (Stigma maydis) on Bone Density and Histology Femur Profiles in Ovariectomized Rats Female Sprague Dawley Strain, Indonesia J. Cancer Chemoprevent, 7(3), 104-109. CrossRef

Vijayanarayana, K., Rodrigues, R.S., Chandrashekhar K.S., and Subrahmanyam E.V., 2007, Evaluation of Estrogenic activity of Alcoholic extracts of rhizhomes of curculigoorchioides, J Ethnopharmacol, 114(2), 241-245. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev11iss2pp75-83

Copyright (c) 2020 Rosa Gloria Sitanggang, Robbani Syahfitri Angkat, Poppy Anjelisa Zaitun Hasibuan

Indexed by:

               

               

      

 

Indonesian Society for Cancer Chemoprevention