Nanoparticle from Medinilla speciosa with Various of Encapsulating Agent and Their Antioxidant Activities Using Ferric Reducing Assay

Rissa Laila Vifta, Fania Putri Luhurningtyas

Abstract


Antioxidants are agents that can reduce free radicals. Parijoto fruit (Medinilla speciosa) contains flavonoids that could act as an antioxidant. However, those flavonoids are water-soluble and show low bioavailability. Nanotechnology is a potential approach to improve the bioavailability of flavonoids from Parijoto fruit. This study was conducted to determine the antioxidant activity of parijoto nanoparticles with variations of the chitosan, alginate, and chitosan/alginate encapsulants. Secondary metabolites of parijoto fruit were using the maceration method. The synthesis of parijoto nanoparticles was conducted using the ionic gelation method with chitosan, alginate, and chitosan/alginate encapsulation. Parijoto nanoparticle size and distribution were characterized using Particle Size Analyzer (PSA). The formation of nanoparticles in colloids was determined as a percent. The antioxidant activity of nanoparticle was evaluated using Ferric Reducing Antioxidant Power (FRAP) method using a UV-Vis spectrophotometer. Chitosan encapsulation produced nanoparticles with a size of 269.3 nm, pdI 0.372 and transmittance 99.379%. Alginate encapsulation produced a particle size of 366.4 nm, pdI 0.589 and transmittance 99.690%. The combination of chitosan/alginate encapsulants produced a particle size of 187.00 nm, pdI 0.239 and transmittance 99.894%. Parijoto nanoparticles obtained from chitosan, alginate, and chitosan/alginate encapsulant showed strong antioxidant powers indicated by IC50 values 2.442±0.047 ppm, 3.175±0.169 ppm and 2.115±0.045 ppm, respectively. Altogether, our study shows that parijoto nanoparticles are potent as antioxidant agents.

Keywords: Alginate, antioxidant, chitosan, FRAP, Medinilla speciosa, nanoparticle


Full Text:

PDF

References


Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G. and Lightfoot, D.A., 2017, Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts, Plants, 6(4), 42. CrossRef

Amari, N.O., Bouzouina, M., Berkani, A. and Lotmani, B., 2014, Phytochemical screening and antioxidant capacity of the aerial parts of Thymelaea hirsuta L., Asian Pacific Journal of Tropical Disease, 4(2), 104-109. CrossRef

Arifin, B. and Ibrahim, S., 2018, Structure, Bioactivity and Antioxidant of Flavonoid, Jurnal Zarah, 6(1), 21–29. CrossRef

Ayumi, D., Sumaiyah, S. and Masfria, M., 2018, The preparation and characterization of ethanol extracts of dragon tail leaves (Rhaphidophora pinnata (L.f.) Schott) using the ionic gelation method, Talenta Conference Series: Tropical Medicine (TM), 1(3), 29–33. CrossRef

Charernsriwilaiwat, N., Rojanarata, T., Ngawhirunpat, T., Sukma, M. and Opanasopit, P., 2013, Electrospun chitosan-based nanofiber mats loaded with Garcinia mangostana extracts, International Journal of Pharmaceutics. Elsevier B.V., 452(1–2), 333–343. CrossRef

Galvez, M.A.C., 2015, Evaluation of DPPH Free Radical Scavenging Activity and Phytochemical Screening of Selected Folkloric Medicinal Plants in Tinoc, Ifugao, Cordillera Administrative Region, Philippines, International Journal of Scientific and Research Publications, 5(12), 440-445.

Irma, L. and Suryanto, E., 2016, Pengaruh Lama Perendaman Terhadap Aktivitas Antioksidan dari Empelur Sagu Baruk (Arenga microcharpha), Chem. Prog., 9(1), 25–34. CrossRef

Kurniasari, D. and Atun, S., 2017, The preparation and characterization of fingerroot (Boesenbergia pandurata) etanol extract nanoparticles with various citosan compotition, Jurnal Sains Dasar, 6(1), 31. CrossRef

Martien, R., Adhyatmika, Irianto, I.D.K., Farida, V. and Sari, D.P., 2012, Technology developments nanoparticles as drug delivery systems, Majalah Farmaseutik, 8(1), 133–144. CrossRef

Maryam, S., Baits, M. and Nadia, A., 2015, The Determination of Antioxidant Activity of Moringa Oleifera Lam. Moringa Leaf Ethanol Extract Using FRAP (Ferric Reducing Antioxidant Power) Method, Jurnal Fitofarmaka Indonesia, 2(2), 115–118. CrossRef

Munin, A. and Edwards-Lévy, F., 2011, Encapsulation of natural polyphenolic compounds, a review, Pharmaceutics, 3(4), 793-829. CrossRef

Ningsih, N., Yasni, S. and Yuliani, S., 2017, Nanoparticle of Red Mangosteen Peel Extract Synthesis and the Functional Characteristic of its Encapsulated Product, Jurnal Teknologi dan Industri Pangan, 28(1), 27–35. CrossRef

Pieroni, L.G., Rezende, F.M.D., Ximenes, V.F. and Dokkedal, A.L., 2011, Antioxidant activity and total phenols from the methanolic extract of Miconia albicans (Sw.) Triana leaves, Molecules, 16(11), 9439-9450. CrossRef

Sa’adah, H., Nurhasnawati, H. and Permatasari, V., 2017, Effect of the extraction method on the concentration of flavonoids ethanol extract of onion dayak bulbs (Eleutherine palmifolia (L.) Merr) using spectrophotometry, Borneo Journal of Pharmascientech, 01(01), 1–9.

Sarian, M.N., Ahmed, Q.U., So’ad, M., Zaiton, S., Alhassan, A.M., Murugesu, S., Perumal, V., Mohamad, S., Akilah, S.N., Khatib, A. and Latip, J., 2017, Antioxidant and antidiabetic effects of flavonoids: A structure-activity relationship based study, BioMed Research International, 2017. CrossRef

Shewasinad, A., Bhoumik, D., Hishe, H.Z. and Masresha, B., 2019, Antidiabetic Activity of Methanol Extract and Fractions of Thymus schimperi Ronniger Leaves in Normal and Streptozotocin Induce Diabetic Mice, Iranian J Pharmacol Ther., 1, 8.

Tahir, M., H., A. C. and Widiastuti, H. 2016. Uji Aktivitas Antioksidan Buah Semangka (Citrullus lanatus) dengan Metode FRAP, As-syifaa Jurnal Farmsasi, 08(01), pp. 31–38.

Taurina, W., Sari, R., Hafinur, U.C., Wahdaningsih, S. and Isnindar, I., 2017, Optimization of Stirring Speed And Stirring Time Toward Nanoparticle Size of Chitosan-Siam Citrus Peel (Citrus nobilis L. var Microcarpa) 70% Ethanol Extract, Majalah Obat Tradisional (Traditional Medicine Journal), 22(1), 16-20. CrossRef

Tusanti, I., Johan, A. and Kisdjamiatun, R.A., 2014, Sitotoksisitas in vitro ekstrak etanolik buah parijoto (Medinilla speciosa) terhadap sel kanker payudara T47D, Jurnal Gizi Indonesia, 2(2), 53–58. CrossRef

Vifta, R.L. and Advistasari, Y.D., 2018, Pytochemical Screening, Characterization, and Determination of Total Flavonoids Extracts and Fractions of Parijoto Fruit (Medinilla speciosa B.), In Prosiding Seminar Nasional Unimus (Vol. 1).

Vijayalakshmi, M. and Ruckmani, K., 2016, Ferric reducing anti-oxidant power assay in plant extract, Bangladesh Journal of Pharmacology, 11(3), 570-572. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev11iss1pp22-29

Copyright (c) 2020 Rissa Vifta

Indexed by:

               

               

      

 

Indonesian Society for Cancer Chemoprevention