Growth of NIH 3T3 Fibroblast Cells Exposed to Carbonated Hydroxyapatite with Incorporated Propolis

Puspaneka Wijayanti, Sri Pramestri Lastianny, Suryono Suryono

Abstract


Carbonated hydroxyapatite is frequently used as bone graft material in dentistry. It is highly biocompatible, has osteoconductive properties, and functions as a drug delivery system. Propolis is a natural product from bees that has antibacterial and anti-inflammatory effects and is capable of accelerating wound healing. Incorporating propolis into carbonated hydroxyapatite was expected to enhance the wound-healing process by stimulating fibroblast growth and regenerating alveolar bone in the treatment of periodontitis. The aim of this study was to evaluate the effect of carbonated hydroxyapatite with incorporated propolis on the viability of NIH 3T3 fibroblast cells. This study used three treatment groups [carbonated hydroxyapatite with various concentrations of incorporated propolis (5%, 7.5%, and 10%)] and one control group (carbonated hydroxyapatite with no propolis). An MTT assay was carried out to assess cell viability, and absorbance readings were performed by using an ELISA reader. The data were analyzed by using one-way ANOVA. The results showed significant differences between all groups, and carbonated hydroxyapatite with 10% incorporated propolis has the highest cell viability level of all groups, while the control group has the lowest cell viability. In conclusion, adding propolis to carbonated hydroxyapatite could increase the growth of NIH 3T3 fibroblast cells.

 

Keywords: Carbonated hydroxyapatite, Propolis, NIH 3T3 fibroblast cells, MTT assay


Full Text:

PDF

References


Abdellatif, B., Mohamed, H., Karim, A. and Asma, B., 2014, Radiography Monitoring of Osteoconduction and Osteoinduction of Orthotopic Allograf Autoclaved Covered Propolis, International Journal of Advances in Life Science and Technology, 1(1), 25-31. CrossRef

Al-waili, N., 2018, Mixing two different propolis samples potentiates their antimicrobial activity and wound healing property : A novel approach in wound healing and infection, Veterinary World, 11(8), 1188-1195. CrossRef

Aral, C.A., Kesim, S., Greenwell, H., Kara.M., Cetin, A. and Yakan, B., 2015, Alveolar Bone Protective and Hypoglycemic Effects of Systemic Propolis Treatment in Experimental Periodontitis and Diabetes Mellitus, J Med Food, 18(2), 195–201. CrossRef

Carranzza, F.A., Newman, M.G., Takei, H.H. and Klokkevosts inid, P.R., 2012, Carranza’s Clinical Periodontologi, 11 th ed. St. Louis Missouri:Sounders Elsevier.

Ding, T., Xue, T., Lu,H., Huang, Z. and Sun, J., 2012, Effect of particle size of hydroxyapatite nano particles on its biocompatibility, IEEE Transactions on Nano Bioscience, (11) 4. CrossRef

Darmadi, D. and Mustamsir, E., 2016, The effect of Propolis on Increasing the Number of Osteoblasts and Chondrocytes, and Decreasing the Number of Osteoclasts in Wistar Rats (Rattusnovergicus) wirth Femoral Bone Fracture, Journal of Dental and Medical Sciences, 15(12), 90-95. CrossRef

Fives-Taylor, P.M., Meyer, D. and Mintz, K., 1996, Virulence factors of the periodontopathogen Actinobacillus actinomycetemcomitans, J. Periodontol., 67, 291-297. CrossRef

Guo, Y., Yao, Y., Guo, Y. and Ning, C., 2012, Hydrothermal fabrication of mesoporus carbonated hydroxyapatite microsshperes for a drug delivery system, Microporous and Mesoporous Materials; Elsevier Inc,155, 245-251. CrossRef

Grenho, L., Barros, J., Ferreira, C., Santos V.R., Monteiro, F.J., Ferraz, M.P. and Cortes, M.E., 2015, In Vitro Antimicrobial Activity and Biocompatibility of Propolis Containing Nanohydroxyapatite, Biomed.Mater, 10. CrossRef

Jacob, A., Parolia, A., Pau, A. and Amalraj, F.D., 2015, The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process, BMC Complementary and Alternative Medicine, 15, 294. CrossRef

Li, B. and Wang, J., 2009, Fibroblasts and Myofibroblasts in Wound Healing: Force Generation and Measurement, J Tissue Viability, 20(4), 108–120. CrossRef

Mai, R., Reinstorf, A., Pilling, E., Hlawitschka, M., Jung, R., Gelinsky, M., Schneider, M., Loukota, R., Pompe, W., Eckelt, U. and Stadlinger, B., 2008, Histologic study of incorporation and resorption of a bone cement-collagen composite: an in vivo study in the minipig, Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 105, e9-e14. CrossRef

Marcucci, M.C.,1995, Propolis: chemical composition, biological properties and therapeutic activity, Apidologie, 26, 83–99. CrossRef

Ozan, F., Polat, Z., A., Er, K., Ozan, U. and Deger, O., 2007, Effect of propolis on survival of periodontal ligament cells: new storage media for avulsed teeth, J.Endod, 33, 570-573. CrossRef

Shokrzadeh, M. and Modanloo, M., 2017, An overview of the most common methods for assessing cell viability, J.Res.Med.Dent.Sci., 5(2), 33-41. CrossRef

Silva, R.P., Machado, B.A.S., Barreto, G.D.S., Costa, S.S., Andrade, L.N. and Amaral, R.G., 2017, Antioxidant, antimicrobial, antiparasitic, and cytotoxic properties of various Brazilian propolis extracts, PLoS ONE, 12(3). CrossRef

Sukumar, S. and Drizhal, I., 2008, Review Article: Bone Grafts in Periodontal Therapy, Acta Medica (Hradec Kralove), 51(4), 203-207. CrossRef

Surbakti, A., Oley, M.C. and Prasetyo, E., 2017, Perbandingan antara penggunaan karbonat apatit dan hidroksi apatit pada proses penutupan defek kalvaria dengan menggunakan plasma kaya trombosit, Jurnal Biomedik (JBM), 9(2), 107-114. CrossRef

Suryono, Hasmy, N.S., Pertiwi, T.L., Benyamin, B. and Ismail, A., 2017, Propolis 10%-Gel as a Topical Drug Candidate on Gingivitis, International Journal of Medicine and Pharmacy, 5(1), 12-17. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev11iss2pp54-59

Copyright (c) 2020 Puspaneka - Wijayanti, Sri Pramestri Lastianny, Suryono Suryono

Indexed by:

               

               

      

 

Indonesian Society for Cancer Chemoprevention