Lymphocyte Proliferation Activity MTT -Test of Ethanolic Extract of Pasak Bumi Root (Eurycoma longifolia Jack) On Induced 7,12-Dimethylbenz[A] Antracene (DMBA) Female Sprague Dawley Rat
Abstract
Pasak bumi root (Eurycoma longifolia Jack) contains quasinoid. Quasinoid potentially has antitumor/anticancer, anti-parasitic, and immunostimulatory activity. The aim of this research was to study the lymphocyte proliferation activity MTT-test of ethanol extract of Eurycoma longifolia Jack root on induced by 7,12-dimethylbenz[a] antracene (DMBA) female Sprague Dawley rat. The test was done at 6 groups of 10 SD rat each. Each groups was administered orally with DMBA (Group I) dose of 20 mg/kg bw, corn oil (Group II), baseline (Group III), and ethanol extract of pasak bumi root dose 12.6; 25.2; and 50.4 mg/kg bw (Group IV,V,and VI). Ethanol extract is given for 5 weeks. At the second week five rats were sacrificed to isolate the lymphocytes of spleen then rats are injected orally with DMBA 2 times a week for 5 weeks. At the eight until twenty fourth week all groups were just administered by aquades. Measurement of spleen relative weight and isolation of the lymphocyte spleen were done at second week and the last of experiment. Lymphocyte proliferation activity were measured by MTT-reduction method. Data were analyzed statistically by analyzes of variance (anova) continued by LSD test and Kruskall Wallis continued by Mann Whitney test. The result showed that limphocyte proliferation activity MTT of ethanol extract of E. longifolia root doses of 12.6; 25.2; and 50.4 mg/kg bw for 14 days prior to DMBA-induced are 339.35 ± 8.43;61.20 ± 6.27;310.28 ± 6.81 and dose 50,4 mg/kg bw after DMBA-induced is 122.108 ± 11.426 .
Keyword: E. longifolia root, spleen, lymphocyte cell, 7,12-Dimetilbenz (a) antracene (DMBA)
Full Text:
PDFReferences
Akrom, 2004, Cellular immune activity of Male Swiss Mice Infected Plasmodium berghei with The Ethanol Extract Phylantus niruri, Research Report, Fac. of Pharmacy, Ahmad Dahlan University, Yogyakarta.
Ang, H.H. and Ngai, T.H., 2001, Aphrodisiac Evaluation In Non-Copulator Male Rats After Chronic Administration of Eurycoma Longifolia, Fundam. Clin. Pharmacol., 15(4), 265-268. Link
Dandekar, S., Sukumar, S., Zaeld, H., Yuong, L., J.T., Cardiff, R.D., 1986, Spesific Activation of The Cellular Harvey-ras Oncogen in Dimethilbenzantracene-Induced Mouse Mammary Tumors, Mol. Cell. Biol., 6(11), 4104-4108. Link
Hargono, D, Winarno, M. W. and Werawati, A., 2000, Effect of Gynura Ngokilo Leaf Juice (Procumbers Lour Meer) on Mice againts Immune System Activity, Cermin Dunia Kedokteran, 127.
Ikawati, Z, 2006, Pengantar Farmakologi Molekuler, Yogyakarta: Gadjah Mada University Press.
Jiwajinda, S., Santisopasri, V., Murakami, A., Hirai, N. and Ohigashi, H., 2002, Quassinoid from Eurycoma longifolia as Plant Growth Inhibitors, Phytochemistry, 58(6), 959-962. Link
Kresno, S. B., 2001, Imunologi: Diagnosis dan prosedur Laboratorium, 4th Edition, Medi, Jakarta.
Kuo, P.C., Shi, L.S., Damu, A.G., Su, C.R., Huang, C.H., Ke, C.H., et al., 2003, Cytotoxic and Antimalarial Beta-carboline Alkaloids from the Roots of Eurycoma longifolia, J. Nat Prod., 66(10), 1324-1327. CrossRef
Miyata, M., Kudo, G., Lee, Y.H., Yang, T.J., Gelboin, H.V., Fernandez-Salguero, P., Kimura, S., et al., 1999, Targeted Disruption of the Microsomal Epoxide Hydrolase Gene, J. Biol. Chem., 274(34), 23963-23968. Link
Shalini, J. and Hariom, Y., 2009, Cell Proliferation Assay, Protocol Online.
Supriyadi, H,M. and Taryono., 2001, Pasak bumi (Eurycoma longifolia Jack) Plant Drug Indonesia, Use and Medicinal Properties, Jakarta: Pustaka Populer Obor.
Tjindarbumi, D. and Mangunkusumo, R., 2002, Cancer in Indonesia, Present and Future, Jpn J. Clin. Oncol.,32(1), 17-21. Link
Underwood, J.C.E., 1993, General and Systemic Pathology, 2nd edition., Jakarta : EGC.
DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev2iss1pp187-192
Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention
Indexed by:
Indonesian Society for Cancer Chemoprevention
