Cardioprotective Effect of Kelor (Moringa oleifera) Leaf Ethanolic Extract against Doxorubicin-Induced Cardiotoxicity in Rats

Fikriansyah Fikriansyah, Mentari Widiastuti, Nindi Wulandari, Prisnu Tirtanirmala, Retno Murwanti


The usage of doxorubicin (DOX) as an anticancer drug in cancer patient may cause several side effects. One of that is cardiotoxicity by inducing the expression of nitric oxide synthase which may release nitric oxide (NO) resulting reactive oxygen species (ROS) in the cardiac. DOX needs to be combined with antioxidant since it could supressed ROS in the cardiac and reduce cardiomyopathy. Kelor (Moringa oleifera) is known as the source of antioxidant. This study aim to observe the treatment effects of ethanolic extract of kelor (EEK) on histopathology profile and concentration of NO in rats cardiac. The result from the hematoxylin-eosin staining showed that EEK improved the histopathology profile of rats’ cardiac. Compared with the DOX-only treatment, the structure of cardiac muscle cells treated by ethanolic extract of kelor is more well-arranged and the cells’ nucleus still visible. Concentration of NO was measured by cardiac puncture method. The result showed that the concentration of NO was decrease in line with increasing dose levels of EEK in combination with DOX. But at rats only given with EEK, the concentration of NO is quite high. In conclusion, EEK could be a cochemotherapy agent by reducing the cardiotoxicity effect of DOX.

Keywords : doxorubicin, Moringa oleifera, nitric oxyde, histopathology


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Arafa H.M., Abd-Ellah M.F. and Hafez H.F., 2005, Abatement by Naringenin of Doxorubicin-Induced Cardiac Toxicity in Rats, J. Egypt. Cancer Inst., 17(4), 291-300. Link

Bustová, I., 2009, Risk of Cardiotoxicity of Combination Treatment Radiotherapy and Chemotherapy of Locally Advanced Breast Carcinoma Stage III, Klin. Onkol., 22(1),17-21. Link

De Beer, E.L., Bottone, A.E. and Voest, E.E., 2001, Doxorubicin and Mechanical Performance of Cardiac Trabeculae after Acute and Chronic Treatment: a Review, Eur. J. Pharmacol, 415(1), 1-11. Link

Fogli, S., Nieri, P. and Breschi, M.C., 2004, The Role of Nitric Oxide in Anthracycline Toxicity and Prospects for Pharmacologic Prevention of Cardiac Damage, FASEB. J., 18(6), 664-675. CrossRef

Frias, M.A., Lang, U., Gerber-Wicht, C. and James, R.W., 2009, Native and Reconstituted HDL Protect Cardiomyocytes from Doxorubicin-induced Apoptosis, Cardiovasc. Res., 85(1), 118-126. CrossRef

Garner A.P., Paine M.J., Chinje E.C., Ortize P., Strat Ford I.J., et al., 1999, Nitric Oxide Synthases Catalyse the Activation of Redox Cycling and Bioreductive Anticancer Agents, Cancer Res., 59(8), 1929-1934. Link

Montano, Calderon J.M., Burgos-Moron E., Perez-Guerrero C. and Lopez- Lazaro M., 2011, A Review on Dietary Flavonoid Kaempferol, Mini. Rev. Med. Chem., 11(4), 298-344. Link

Palmer, R.M., Ashton, D.S. and Moncada, S., 1988, Vascular Endothelial Cells Synthesize Nitric Oxide From L. arginine, Nature., 333(6174), 664-666. CrossRef

Schildknecht, S., Pape, R., Muller, N., Robotta, M., Marquardt, A., Burkle, A., et al., 2011, Neuroprotection by Minocycline caused by Direct and Spesific Scavenging of Peroxynitrite, J. Biol. Chem., 286(7), 4991-5002. CrossRef

Sharma, G., Tyagi, A.K., Singh, R.P., Chan, D.C.F. and Agarwal, R., 2004, Synergistic Anticancer Effect of Grape Seed Extract and Conventional Cytotoxic Against Human Breast Carcinoma Cells, Breast Cancer Res. Treat., 85(1), 1-12. CrossRef

Siddhuraju, P. and Becker, K., 2003, Antioxidant Properties of Various Solvent Extracts of Total Phenolic Constituents from Three Different Agroclimatic Origins of Drumstick Tree (Moringa oleifera Lam.) Leaves, J. Agric. Food Chem., 51(8), 2144-2155. CrossRef

Singal, P.K., Li, T., Kumar, D., Danelisen, I. and Iliskovic, N., 2000, Adriamycin-induced Heart Failure: Mechanism and Modulation, Mol. Cell Biochem, 207(1-2), 77–86. Link

Tyagi, A.K., Agarwal, C., Chan, D.C.F. and Agarwal, R., 2004, Synergistic Anti Cancer Effect of Silibinin with Conventional Cyotoxic Agents Doxorubicin, Cisplatin, and Carboplatin against Human Breast Carcinoma MCF-7 and MDA-MB468 Cells, Oncol. Rep., 11(2), 493-499. Link


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