Annona muricata's Leaves Ethanolic Extract Enhance p53 Expression in Rat Hepatic Cells Induced by Dimethylbenz(a)Anthracene (DMBA)

Rosa Adelina, Intan Sari Oktoberia, Putri Reno Intan

Abstract


Annona muricata or soursop contains acetogenin which can induce apoptosis in vitro and has cytotoxic effect. The study has been done to examine soursop leaves extract’s potency in vivo especially to induce apoptosis in 7,12-Dimethylbenz[a]anthracene (DMBA)-induced Sprague Dawley rats. The induction process was done twice a week for five weeks. The soursop leaves extract were given in three dosages, that are 200, 400, and 800 mg/kgBW for seventeen days after two weeks DMBA induction. Data analysis showed that Annona muricata leaves ethanolic extract could enhance p53 pathway significantly with a dose independent manner in DMBA-induced rats.

Keywords: Proapoptosis,  Annona muricata leaves ethanolic extract, hepatoma


Full Text:

PDF

References


American Cancer Society [editorial], 2013, Cancer Facts and Figures 2013, Website, http://www.cancer.org/research/cancerfactsfigures/cancerfactsfigures/cancer-facts-figures-2013. Link

Bykov, V.J., Issaeva, N., Shilov, A., Hultcrantz, M., Pugacheva, E., Chumakov, P., et al., 2002, Restoration of the Tumor Suppressor Function to Mutant p53 by a Low- Molecular-Weight Compound, Nat. Med., 8(3), 282–288. CrossRef

Chih, H., Chiu, H.F., Tang, K.S., Chang, F.R. and Wu, Y.C., 2001, Bullatacin, a Potent Antitumour Annonaceous Acetogenin, Inhibits Proliferation of Human Hepatocarcinoma Cell Line 2.2.15 by Apoptosis Induction, Life Sci., 69(11), 1321–1331. Link

Das, S., Raj, L., Zhao, B., Kimura, Y., Bernstein, A., Aaronson, S.A., et al., 2007, Hzf Determines Cell Survival Upon Genotoxic Stress by Modulating p53 Transactivation, Cell., 130(4), 624–637. CrossRef

Gibbs, J.B., 2000, Anticancer Drug Targets: Growth Factor and Growth Factor Signaling, J. Clin. Invest., 105(1), 9-13. CrossRef

Gudkov, A.V. and Komarova, E.A., 2010, Pathologies Associated with the p53 Response, Cold Spring Harb. Perspect Biol., 2, a001180. CrossRef

Halasova, E., Adamkov, M., Kavcova, E., Matakova, T., Musak, L., Vybohova, D., et al, 2009, Expression of Anti-Apoptotic Protein Surveivin and Tumor Supressor p53 Protein in Patients with Pulmonary Carcinoma, Eur. J. Med. Res., 14(4), 97-100. CrossRef

Issaeva, N., Bozko, P., Enge, M., Protopopova, M., Verhoef, L.G., Masucci, M., et al., 2004, Small Molecule RITA Binds to p53, blocks p53-HDM-2 Interaction and Activates p53 Function in Tumors, Nat. Med., 10(12), 1321–1328. CrossRef

Kim, G., Zeng, L., Alali, F., Rogers, L.L., Wu, F., McLaughlin, J.L., et al., 1998, Two New Mono-Tetrahydrofuran Ring Acetogenins, Annomuricin E and Muricapentocin, from the Leaves of Annona muricata, J. Nat. Prod., 61(4), 432-436. CrossRef

King, R.J.B., 2000, Cancer Biology, 2nd Edition., London: Pearson Eduation Limited.

Looi, M.L., Dali, A.Z.H.M., Ali, S.A.M., Ngah, W.Z.W. and Yusof, Y.A.M., 2008, Expression of p53, Bcl-2, and Ki-67 in Cervical Intraepithelial Neoplasia and Invasive Squamous Cell Carcinoma of the Uterine Cervix, 30(2), Website, http://www.aqch.com/feature/2008/feature042008.php. Link

Midgley, C.A., Desterro, J.M., Saville, M.K., Howard, S., Sparks, A., Hay, R.T., et al, 2000, An N-terminal p14ARF Peptide Blocks Mdm2-Dependent Ubiquitination In Vitro and Can Activate p53 In Vivo, Oncogene., 19(19), 2312–2323. CrossRef

National Institute of Health Research and Development (NIHRD), 2013, Penyajian Pokok-Pokok Hasil Riset Kesehatan Dasar 2013, Jakarta: Ministry of Heath.

Parkin, D.M.,2001, Global Cancer Statistics in The Year 2000, Lancet Oncol., 2(9), 533-543. CrossRef

Pfizer, 2013, Pfizer Fact: The Burden of Cancer in Asia, Website, http://www.pfizer.com/files/products/cancer_in_asia.pdf. Link

Soussi, T., Dehouche, K. and Beroud, C., 2000, p53 Website and Analysis of p53 Gene Mutations in Human Cancer: Forging a Link Between Epidemiology and Carcinogenesis, Hum. Mutat., 15(1), 105–113. CrossRef

Tanaka, T., Ohkubo, S., Tatsuno, I. and Prives, C., 2007, hCAS/CSE1L Associates with Chromatin and Regulates Expression of Select p53 Target Genes, Cell, 130(4), 638–650. 10.1016/j.cell.2007.08.001">CrossRef

Thuluvath, P.J., Choti, M., Geschwind, J.F., Norwitz, L. and Kalloo, A.N., 2006, Liver Cancer, Website, https://gastro.nts.jhu.edu. Link

Tyagi, A.K., Agarwal, C., Chan, D.C.F. and Agarwal, R., 2004, Synergistic Anticancer Effects of Silibini with Conventional Cytotoxic Agents Doxorubicin, Cisplatin and Carboplatin Against Human Breast Carscinoma MCF-7 and MDA-MB468 Cells, Oncol. Rep., 11(2), 493-499. Link

Umadevi, M., Kumar, K.P.S., Bhowmik D, and Duraivel S, 2013, Traditionally Used Anticancer Herbs in India, J. Med. Plants. Stud., 1(3), 56-74. Link

Vassilev, L.T., 2005, p53 Activation by Small Molecules: Application in Oncology, J. Med. Chem., 48(14), 4491–4499. CrossRef

Walaszek, Z., Hanausek, M. and Slaga, T.J., 2004, Mechanisms of Chemoprevention, Supplement American College of Physicians, 125(5), 128-133.

Yayasan Kanker Indonesia, 2012, YKI-Jakarta Race, Website, https://yayasankankerindonesia.org/2012/yki-jakarta-race/. Link




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev4iss1pp483-487

Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:

               

               

      

 

Indonesian Society for Cancer Chemoprevention