Synergistic Effect of Cinnamon Essential Oil (Cinnamomum burmannii) and Doxorubicin on T47D Cells Correlated with Apoptosis Induction

Etyk Yunita Anjarsari, Nita Kristina, Yonika Arum Larasati, Dyaningtyas Dewi Pamungkas Putri, Edy Meiyanto

Abstract


Cinnamon  (Cinnamomum burmannii)  shows  anticancer activity  in  several  types  of  cancer  cells.  The aim of this study is to  observe the  cytotoxic  activity  of  cinnamon essential oil (CEO) solely and its combination with doxorubicin,  also the  ability  of  the  combination to induce apoptosis on T47D breast cancer cells. The CEO was prepared through distillation of dry cinnamon bark. Cytotoxic  assay  was performed by using MTT assay and apoptosis determination was done by using double staining with ethidium bromide and acridine orange. The result showed that CEO exhibited cytotoxic effect on T47D cells with IC50 values of 75 µg/ml.  Moreover, CEO showed synergist effect with doxorubicin. The lowest combination index (CI) with CI values of 0,37 was obtained by combination of doxorubicin-CEO  37,5  µg/ml-1,25  µM.  Treatment with CEO solely and its combination with doxorubicin showed apoptosis induction on T47D cells. The results of this  study  indicate  the potency of CEO  to  be  developed  as co-chemotherapeutic  agent  of  doxorubicin on breast cancer. 

Keywords:  cinnamon essential oil, doxorubicin, T47D cells, combination cytotoxic


Full Text:

PDF

References


American Cancer Society, Breast Cancer Facts & Figures 2013-2014, Atlanta: American Cancer Society, Inc. 2013.

Drummond, C., 2007, The Mechanism of Anti-tumour Activity of the DNA Binding Agent SN 28049, Thesis, University of Auckland, New Zealand.

Ka, H., Park, H.J., Jung, H.J., Choi, J.W., Cho, K.S., Ha, J. and Lee, K.T., 2003, Cinnamaldehyde Induces Apoptosis by ROS-mediated Mitochondrial Permeability Transition in Human Promyelocytic Leukemia HL-60 cells, Cancer Lett., 196(2), 143-152. CrossRef

Kasibhatla, S., Amarate-Mendes, G.P., Finucane, D., Bruner, T., Bossy-Wetzel, E. and Green, D.R., 2006, Acridine Orange/Ethidium Bromide (AO/EB) Staining to Detect Apoptosis, CHS Protoc., 2006(3). CrossRef

Kwon, H.K., Hwang, J.S., So, J.S., Lee, C.G., Sahoo, A., Ryu, J.H., et al., 2010, Cinnamon Extract Induces Tumor Cell Death Through Inhibition of NFkB and AP1, BMC Cancer 2010, 10(1), 392. CrossRef

Lee, C.W., Hong, D.H., Han, S.B., Park, S.E., Kim, H.K., Kwon, B.M., et al., 1999, Inhibition of Human Tumor Growth by 2’- Hydroxyl- and 2’- Benzoyl-oxycinnamaldehydes, Planta Med., 65(3), 263-266. CrossRef

Mizutani, H., Oikawa, S.T., Hiraku, Y., Kojima, M. and Kawanishi, S., 2005, Mechanism of Apoptosis Induced by Doxorubicin Through the Generation of Hydrogen Peroxide, Life Sci., 76(13), 1439-1453. CrossRef

Pahl, H.L., 1999, Activators and Target Genes of Rel/NFκB Transcription Factors, Oncogene, 18(49), 6853-6866. CrossRef

Reynolds, C.P. and Maurer, B.J., 2005, Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models, Methods in Molecular Medicine, Methods Mol. Med., 110, 173-183. CrossRef

Singh, G., Maurya, S.S., Delampasona, M.P. and Catalan, C.A.N., 2007, A Comparison of Chemical, Antioxidant and Antimicrobal Studies of Cinnamon Leaf and Bark Volatile Oils, Oleoresins and Their Constituents, Food Chem. Toxicol., 45(9), 1650-1661. CrossRef

Smith, L., Watson, M.B., O'Kane, S.L., Drew, P.J., Lind, M.J. and Cawkwell, L., 2006, The Analysis of Doxorubicin Resistance In Human Breast Cancer Cells Using Antibody Microarrays, Mol. Cancer Ther., 5(8), 2115-2120. CrossRef

Tyagi, A.K., Agarwal, C., Chan, D.C.F. and Agarwal, R., 2004, Synergistic Anti Cancer Effects of Silibinin with Conventional Cytotoxic Agents Doxorubicin, Cisplatin, and Carboplatin Against Human Breast Carcinoma MCF-7 and MDA-MB468 Cells, Oncol. Rep., 11(2), 493-499. Link

Ueda, J.Y., Tezuka, Y., Banskota, A.H., Tran, Q.L., Tran, Q.K., Harimaya, Y., et al., 2002, Antiproliferative Activity of Vietnamese Medicinal Plants, Biol. Pharm. Bull, 25(6), 753-760. Link

Wattanapitayakul, S.K., Chularojmontri, L., Herunsalee, A., Charuchong- kolwongse, S., Niumsakul, S. and Bauer, J.A., 2005, Screening of Antioxidants from Medicinal Plants for Cardioprotective Effect Against Doxorubicin Toxicity, Basic Clin. Pharmacol. Toxicol, 96(1), 80-87. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev4iss1pp450-456

Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:

               

               

 

Indonesian Society for Cancer Chemoprevention