Elettaria cardamomum Distillate Increases Cellular Immunity in Doxorubicin Treated Rats

Rikat Raksamiharja, Khairunnisa Sy, Meirizky Zulharini S., Annisa Novarina, Ediati Sasmito


Doxorubicin is one of chemotherapeutic agent used for cancer therapy. However, doxorubicin usage causes some adverse effects, such as lymphocyte, CD4+ and CD8+ cells number. Therefore, the co-chemotherapeutic agent is required to reduce the imunosuppression effect. Cardamom (Elettaria cardamomum) contains terpenoid 1,8 cineol. This research aimed to know the effect of Elettaria cardamomum distillate (ECD) in combination with doxorubicin on Sprague Dawley rat’s hematology profile and the amount of CD4+ and CD8+ cells. The experiment was done for 13 days using 6 groups of rats: I doxorubicin (dox) 15 mg/kg BW; II dox + ECD5 mg/kg BW; III dox + ECD 50 mg/kg BW; IV dox + ECD 100 mg/kg BW; V ECD 100 mg/kg BW; VI control without treatment. The hematology profile and the amount of CD4+ and CD8+ were counted before and after treatment using flowcytometer. The results show that ECD increases the amount of lymphocyte, white blood, CD4+ and CD8+ cells in dose dependent manner in doxorubicin treated rats. Based on the data, it can be concluded that ECD is potential to be developed as immunostimulant agent for chemotherapy.

Keywords: Cardamom (Elettaria cardamomum), immunostimulant, hematology, CD4+, CD8+

Full Text:



Aggarwal, B.B., Shishodia, S., Sandur, S.K., Pandey, M.K. and Sethi, G., 2006, Inflammation and cancer: how hot is the link?, Biochem. Pharmacol., 72(11), 1605-1621. CrossRef

Bustova, I., 2009, Risk of Cardiotoxicity of Combination Treatment Radiotherapy and Chemotherapy of Locally Advanced Breast Carcinoma Stage III, Klin. Onkol., 22(1), 17-21. Link

Cho, K.H., 2012, 1,8 Cineole Protected Human Lipoproteins from Modification by Oxidation and Glycation and Exhibited Serum Lipid-Lowering and Anti-Inflammatory Activity in Zebafish, BMB Rep., 45(10), 565-570. Link

Gochev, V., Girova, T., Stoilova, I., Atanasova, T., Nenov, N., Stanchev, V., et al., 2012, Low Temperature Extraction of Essential Oil Bearing Plants by Liquefied Gases. 7. Seeds from Cardamom (Elettaria cardamomum (L.) Maton), J. BioSci. Biotech., 1(2), 135-139. Link

Hassan, S.B., Muhtasib H.G., Goransson, H. and Larsson, R., 2010, Alpha Terpineol: A Potential Anticancer Agent which Acts through Suppressing NF-κB Signalling, Anticancer Res., 30(6), 1911-1920. Link

Huang, Y., Fang, J., Hung, C., Wu, P. and Tsai, Y., 1999, Cyclic Monoterpene Extract from Cardamom Oil as a Skin Permeation Enhancer for Indomethacin: In Vitro and In Vivo Studies, Biol. Pharm. Bull., 22(6), 642–646. Link

Jakóbisiak, M., Lasek, W. and Gołab, J., 2003, Natural Mechanisms Protecting Against Cancer, Immunol. Lett., 90(2-3), 103-122. Link

Majdalawieh, A.F. and Carr, R.I., 2010, In vitro investigation of the potential immunomodulatory and anti-cancer activities of black pepper (Piper nigrum) and cardamom (Elettaria cardamomum), J. Med. Food, 13(2), 371-381. CrossRef

Moteki, H., Hibasami, H., Yamada, Y., Katsuzaki, H., Imai, K. and Komiya, T., 2002, Specific Induction of Apoptosis by 1,8-cineole in Two Human Leukemia Cell Lines, But Not a in Human Sstomach Ccancer Cell Line, Oncol. Rep., 9(4), 757–760. Link

Patel, D., Shukla, S. and Gupta, 2007, Apigenin and Cancer Chemoprevention: Progress, Potential, Promise (Review), Int. J. Oncol., 30(1), 233-245. Link

Serafino, A., Vallebona, P.S., Andreola, F., Zonfrillo, M., Mercuru, L., Federici, M., et al., 2008, Stimulatory Effect of Eucaliptus Essential Oil on Inante Cell-Mediated Immune Response, BMC Immunol., 9, 17. CrossRef

Uspenskaya, Y.A., Mikhutkina, S.V.,. Taksanova, E.I., Popova, N.N., Olovyannikova, R.Y. and A. B. Salmina, 2004, Induction of Apoptosis in Bone Marrow Cells is Mediated via Purinergic Receptors, Bull. Exp. Biol. Med., 138(2), 114-118. Link

Wattenberg, L., 1992, Inhibition of Carcinogenesis by Minor Dietary Constituents, Cancer Res., 52(7), 2085–2091. Link

Zhang, X.Y., Li, W.G., Wu, Y.J. and Gao, M.T., 2005, Amelioration of Doxorubicin-Induced Myocardial Oxidative Stress and Immuosuppression by Grape Seed Proanthocyanidins in Tumour-bearing Mice, J. Pharm. Pharmacol., 57(8), 1043-1052. CrossRef

DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev3iss3pp437-443

Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:




Indonesian Society for Cancer Chemoprevention