Ethanolic Extract of Papaya (Carica papaya) Leaves Improves Blood Cholesterol Profiles and Bone Density in Ovariectomized Rats

Raisatun Nisa Sugiyanto, Rahmi Khamsita, Ratna Asmah Susidarti

Abstract


Bone loss and disturbance in the blood cholesterol profiles modulation are two effects caused by menopauses syndromes. As the estrogen concentration in the body decreased drastically, menopause women need the replacement of estrogen to keep the regulation of several physiological functions in the body, such as bone generation and cholesterol regulation in a good condition. Phytoestrogen in Carica papaya leaves, such as quercetin, could be one of the potential agents for the estrogenic effect. The aim of this study is to know the effects of papaya leaf extract (PLE) on the blood cholesterol profiles and bone density in ovariectomized rats. Thirty six female Sprague Dawley rats divided into six groups.  The groups were sham-treated ovx (S-OVX), ovariectomized rats (OVX), CMC-Na control (OVX+CMC-Na), positive control (OVX+Estradiol), and the PLE treatment groups dose 750 mg/kgBW (OVX+750mg/kgBW) and dose 1000 mg/kgBW (OVX+1000 mg/kgBW). Administrations of PLE were done in three weeks orally and estradiol administrated intraperitonially. In the end of the treatment, the blood sample of tested animals was collected for the blood cholesterol determination (LDL, HDL, triglyceride, and total cholesterol) and the femur bones were examined for the bone density. Based on the results, PLE dose of 750 mg/kgBW a day in ovariectomized rats showed estrogenic effects in modulating blood cholesterol profile by lowering total cholesterol levels. Meanwhile, PLE dose of 1000 mg/kgBW significantly increased the bone density (p<0.05). Thus, PLE is potential to overcome the negative effects of post-menstrual women especially in the cholesterol blood profiles and bone density.

Keywords : Carica papaya, phytoestrogen, bone density, blood cholesterol, ovariectomized rats


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References


Canini, A., Alesiani, D., D’Arcangelo. and G., Tagliatesta, P., 2007, Gas Chromatography–Mass Spectrometry Analysis of Phenolic Compounds from Carica papaya L. leaf, J. Food Compost. Anal., 20(7), 584-590. CrossRef

Gent, J. and Braakman, I., 2004, Low-density Lipoprotein Receptor Structure and Folding, Cell Mol. Life Sci., 61(19-20), 2461-70. CrossRef

Gruber, C.J., Tschugguel, W., Schneeberger, C. and Huber, J.C., 2002, Production and Action of Esterogens, N. Engl. J. Med., 346(5), 340-350. CrossRef

Harnish, D.C., Evans, M.J., Scicchitano, M.S., Bhat, R.A. and Karanthanasis, S.K., 1998, Estrogen Regulation of the Apolipoprotein Al Gene Promoter through Transcription Cofactor Sharing, J. Biol. Chem., 273(15), 9270-9278. CrossRef

Kosnayani, A.S., 2007, Hubungan Asupan Kalsium, Aktivitas Fisik, Paritas, Indeks Massa Tubuh, dan Kepadatan Tulang pada Wanita Pasca Menopause, Thesis, Semarang : Program Doktoral Ilmu Gizi dan Kesehatan Masyarakat UNDIP.

Lundeen, S.G., Carver, J.M., McKean, M.L. and Winneker, R.C., 2007, Characterization of the Ovariectomized Rat Model for the Evaluation of Esterogen Effect on Plasma Cholesterol Levels, Endicronology, 138(4), 1552-1558. CrossRef

Monroe, D.G., Secreto, F.J. and Spelsberg, T.C., 2003, Overview of Estrogen Action in Osteoblasts: Role of the Ligand the Receptor and The Coregulators. J. Musculoskelet. Neuronal. Interact., 3(4), 357-362. Link

Murray, R.K., Granner, D.K., Mayer, P.A. and Rodwell, V.W., 1995, Biokomia Harper, Jakarta: Penerbit Buku Kedokteran EGC.

Prince, R.L. and Draper, C., 2000, Bone and Calcium dalam Lobo, R.A., Kelsey, J., Marcus, R., (eds) Menopause: Biology And Pathobiology, Academic Press, San Diego California.

Ruggiero, J. and Likis, F.E., 2002, Estrogen: Physiology, Pharmacology, and Formulations for Replacement Therapy, J. Midwifery Womens Health., 47(3), 130-138. Link

Whitten, P.L. and Patisaul, H.B., 2001, Cross-Species and Interassay Comparisons of Phytoesterogen Action, Environ. Health Perspect, 109(1), 5-20. Link




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev3iss3pp426-431

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