Antiproliferative Effect of Chloroform Extract of Cangkring Leaves (Erythrina fusca Lour.) and Its Isolates against HeLa Cells

Endah Puspitasari, Endah Dwi Setyowati, Binar Asrining Dhiani, Sugeng Riyanto

Abstract


Cervical cancer is a type of cancer possessing the 3rd highest incidence among women worldwide. Today’s therapies against cancer are still ineffective, thus people are seeking for alternative method. Erythrina fusca Lour. has been used traditionally as anticancer. This experiment is conducted to study the ability of chloroform extract of E. fusca Lour. leaves and its isolates on HeLa cervical cancer cells. The cytotoxicity assay and doubling time assay were done using direct counting method, while the DNA staining assay was done using acrydine orange. The chloroform extract and isolate # 30 showed cytotoxic activity with IC50 of 16 and 5 µg/ml, respectively, while isolate # 2 and isolate # 8 didn’t. The effluent # 30 could not affect HeLa cells growth at the given concentration, but it might promote DNA fragmentation indicating apoptosis induction. The molecular mechanisms underlying these effects still need to be further explored.

Keywords: Erythrina fusca Lour., isolate and effluent of chloroform extract, antiproliferative, cervical cancer


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References


Afiyati, R., 2002, Efek Antiproliferatif Ekstrak Etanol Biji Tanaman Cangkring (Erythrina fusca Lour.) terhadap Sel HeLa, Essay, Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta.

Armanios, M. and Greider, C.W., 2005, Telomerase and Cancer Stem Cells, Cold Spring Harb. Symp. Quant. Biol, 70, 205-208. CrossRef

Bai, L. and Zhu, W.G., 2006, p53: Structure, Function, and Therapeutic Applications, J. Cancer Mol., 2(4), 141-153. https://pdfs.semanticscholar.org/8ed9/f4d5c61dda26c523c197a731982a2b03470b.pdf">CrossRef

Chahar, M.K., Sharma, N., Dobhal, M.P. and Joshi, Y.C., 2011, Flavonoids: a Versatile Source of Anticancer Drugs, Pharmacogn. Rev., 5(9), 1-12. CrossRef

Desaintes, C., Goyat, S., Garbay, S., Yaniv, M. and Thierney, F., 1999, Papillomavirus E2 Induces p53-Independent Apoptosis in HeLa Cells, Oncogene, 18(32), 4583-4545. CrossRef

Di Filippo, M. and Bernardi, G., 2009, The Early Apoptotic DNA Fragmentation Targets a Small Number of Specific Open Chromation Regions, PloS One, 4(4), e5010. CrossRef

Duke, J.A., 1983, Erythrina fusca Lour., Handbook of Energy Crops, Websitehttp://www.hort.purdue.edu/newcrop/ duke_energy/Erythrina_fusca.html, accessed on March 20th, 2013. Link

Field, S.J., Tsai, F.Y., Kuo, F., Zubiaga, A.M., Kaelin, Jr.W.G. Livingston, D.M., et al., 1996, E2F Functions in Mice to Promote Apoptosis and Suppress Proliferation, Cell, 85(4), 549-561. Link

Julia, S., 2001, Uji Sitotoksisitas Fraksi Protein Daun Erythrina fusca Lour. (Cangkring) pada Sel HeLa, Essay, Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta.

Koehn, F.K. and Carter, G.T., 2005, Rediscovering Natural Products as a Source of New Drugs, Discov. Med., 5(26), 159-164. Link

Mallumbres, M. and Barbacid, M., 2009, Cell Cycle, CDKs and Cancer: a Changing Paradigm, Nat. Rev. Cancer, 9(3), 153-166. CrossRef

Meiyanto, E., Sismindari, Candra, L. and Moordiani, 2003, Efek Antiproliferatif Ekstrak Etanol Daun dan Kulit Batang Tanaman Cangkring (Erythrina fusca Lour.) terhadap Sel HeLa, Indones. J. Pharm., 14(3), 124-131. Link

Prayong, P., Barusrux, S. and Weerapreeyakul, N., 2008, Cytotoxic Screening of Some Indigenous Thai Plants, Fitoterapia, 79(7-8), 598-601. CrossRef

Redd, W.H., Montgomery, G.H. and DuHamel, K.N., 2001, Behavioral Intervention for Cancer Treatment Side Effects, J. Natl. Cancer Inst., 93(11), 810-823. Link

Roy, S., Kaur, M., Agarwal, C., Tecklenburg, M., Sclafani, R.A. and Agarwal, R., 2007, p21 and p27 Induction by Silibinin is Essensial for Its Cell Cycle Arrest Effect in Prostate Carcinoma Cells, Mol. Cancer Ther., 6(10), 2696-2707. CrossRef

Sismindari, Yuswanto, A.G., Susanti, L. and Ngolady, D., 2001, Effects of Aqueous and Methanol Extract of Erythrina fusca Lour. on DNA Topoisomerase II, J. Biotechnol., 12, 481-487. Link

WHO/ICO, 2010, Information Centre on HPV and Cervical Cancer (HPV Information Centre), Human Papilomavirus and Related Cancers in World, Summary Report 2010, Webiste, http://apps.who.int/ hpvcentre/statistics/dynamic/ico/SummaryReportsSelect.cfm, accessed on March 20th, 2011. Link




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev3iss1pp358-363

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