Ficus septica Burm. f. Leaves Ethanolic Extract Triggered Apoptosis on 7,12-Dimethylbenz[a]anthracene-Induced Rat Mammary Carcinogenesis Qualitatively

Anindyajati Anindyajati, Andita Pra Darma, Ika Nurjizah, Dita Brenna Septhea, Agung Endro Nugroho

Abstract


Ficus septica Burm. f. ethanolic extract (FEE) shows cytotoxic effects on several cancer cell lines. Our research aimed to investigate the effect of FEE on apoptosis induction and p53 expression against carcinogenesis of 7,12-Dimethylbenz[a]anthracene (DMBA)-induced rat mammary.The research was conducted by comparing both apoptosis induction and p53 expression in DMBA-induced rats that were treated with FEE against control groups. Cells that undergo apoptosis were visualized by Double Staining method with acridine orange and ethidium bromide, while p53 expression was detected by IHC staining. Double staining results showed increased occurrence of apoptotic cells compared to the control groups. IHC staining of p53 did not show significant difference between treatment and control groups. However, FEE was able to repair morphology of cells undergoing carcinogenesis. Thus, we conclude that FEE has an anti-carcinogenic activity on DMBA-induced rat mammary through apoptosis induction without affecting p53 expression. Therefore, the ethanolic extract of Ficus Septica leaves is a potential chemo-preventive agent on breast cancer. Further study on its molecular mechanism needs to be explored.

Keywords: Ficus septica, breast cancer, 7,12-Dimethylbenz[a]anthracene, carcinogenesis, apoptosis, p53


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References


Hanahan, D. and Weinberg, R.A., 2011, Hallmarks of Cancer: The Next Generation, Cell, 144(5), 646-674. CrossRef

Jemal, A., Siegel, R., Ward, E., Hao, Y., Xu, J., Murray, T., et al., 2008, Cancer Statistik 2008, CA Cancer J. Clin., 58(2), 71-96. CrossRef

King, R.J.B., 2000, Cancer Biology, 2nd Edition., London: Pearson Eduation Limited.

Mubarok M.F., Sekti Dewi A. and Ainun W., 2008, Peningkatan Aktivitas Sitotoksik Doxorubicin terhadap Sel MCF-7 menggunakan Ekstrak Etanolik Daun Awarawar (Ficus septica Burm. f.), Proceeding KONGRES ILMIAH XVI ISFI 2008. ISBN : 978-979-95107-6-2. Penerbit : Ikatan Sarjana Farmasi Indonesia.

Nugroho, A.E., Ikawati, M., Hermawan, A., Putri, D.D.P. and Meiyanto, E., 2011, Cytotoxic Effect of Ethanolic Extract Fractions of Indonesia Plant Ficus septica Burm. F. on Human Breast Cancer T47D Cell Lines, Int. J. Phytomed, 3(2011), 216-226. CrossRef

Staerk, D., Lykkeberg, A.K, Christensen, J., Budnik, B.A., Abe, F. and Jaroszewski, J.W., 2002, In Vitro Cytotoxic Activity of Phenanthroindolizidine Alkaloids from Cynanchum vincetoxicum and Tylophora tanakae against Drug-Sensitive and Multidrug Resistant Cancer Cells, J. Nat. Prod., 65(9), 1299-1302. Link

Sun, S.Y., Hail, N. Jr. and Lotan, R., 2004, Apoptosis as a Novel Target for Cancer Chemoprevention, J Natl. Cancer Inst., 96(9), 662-672. Link

Tamimi, R.M., Lagiou, P., Adami, H.O. and Trichopoulos, D., 2002, Prospect for Chemopreventionof Cancer, J. Intern. Med., 251(4), 286-300. Link

Wyllie, A., Donahue, V., Fischer, B.P., Hill, D., Kessey, J. and Manzow, 2000, Cell Death, Roche Diagnostic Cooporation, 2-64.




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev3iss1pp334-338

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