Antiproliferative Effect and Apoptosis Induced in Human Cell Lines by Bruguiera gymnorhiza Barks Methanol Extract

Warsinah Warsinah, Sismindari Sismindari, Ratna Asmah Susidarti

Abstract


The Antiproliferative effects of methanol extract from Bruguiera gymnorhiza (B. gymnorhiza) barks were tested in vitro against three human cell lines: Hela, Raji and Myeloma cells. The extract was found to have antiproliferative effects against Hela, Raji and Myeloma cells with an IC50 value of 133, 504 and 384 µg/mL, respectively. The antiproliferative test was then performed on Hela, Raji, and Myeloma cells. Cytotoxicity assay of the extract was then determined using MTT method. There were some indications of apoptosis, such DNA fragmentation, as assessed by acrydine orange- ethidium bromide staining. These results indicate that extract from B. gymnorhiza barks can induce apoptosis in human cell lines.

Keywords: Antiproliferative, Apoptosis, B. gymnorhiza


Full Text:

PDF

References


Jiang, S., 1977, Dictionary Of Traditional Chenese Crude Drugs, Vol 2, Shanghai Scientific Technologic, Shanghai.

McConkey, D.J., Orrenius, S. Jondal, M., 1990, Agent that Elevate cAMP Stimulate DNA Frgmentation in Thymocytes, J. Immunol., 145(4), 1227-1230. Link

Mimaki, Y., Kanmoto, T., Sashida, Y.,Nishino, A., Satomi, Y. and Nishino H., 1996, Steroidal Saponins from Underground Parts of Chloorophytum Comusum and Inhibitory Activity on Tumor Promoter Induce Phospolipids Metabolism of Hela Cells, Phytocemistry, 41(5), 1495-1410. Link

Pasechnik, V., 2000, Microbial Pathogens And Apoptotics Andicancer Therapy, Expert, Opin. Investig. Drugs, 9(6), 1243-1256. CrossRef

Tsuruo, T., Naito, M., Tomida, A., Fujita, N., Mashima, T., Sakamoto, H., et al, 2003, Molecular Targeting Therapy of Cancer: Drug Resistance, Apoptosis and Survival Signal, Cancer Sci., 94(1), 15-21. Link

Warsinah, Lestari, P. and Trisnowati, 2005, Isolasi Senyawa Bioaktif pada Kulit Batang B. gymnorhiza Sebagai Bahan Andikanker. Laporan Penelitian Dasar.

Wyllie, A.H., Kerr, J.F. and Currie, A.R., 1980, Cell Death; The Significance of Apoptosis, Int. Rev. Cytol., 68, 251-306. Link




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev2iss3pp299-303

Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:

                  

               

 

Indonesian Society for Cancer Chemoprevention