The Cytotoxic Activity of Solanum Nigrum Ethanolic Extract on Widr Human Colon Cancer Cells

Astrid Ayu Maruti, Ilham Augusta F., Dyaningtyas Dewi Pamungkas Putri, Adam Hermawan, Muthi' Ikawati

Abstract


Solanum nigrum L. or Leunca in Indonesia has been traditionally used as a herbal plant, which is believed to have anti-tumor properties, although the mechanism for the activity remains unknown. The resecarch aim to examine the cytotoxic effect of the ethanolic extract of Solanum nigrum on WiDr human colon cancer cells. In this study, we prepared an ethanol extract from herb of Solanum nigrum and investigated the mechanism involved in its growth-inhibitory effect on WiDr human colon cancer cells. Herbs of Solanum nigrum dry powder is extracted with 70% ethanol then added into the WiDr cell culture in 96 wells plate in various concentration : 50, 100, 250, and 500 µg/ml. Cytotoxicity of the Solanum nigrum ethanolic extract  was analyzed with MTT assay on WiDr human colon cancer cell lines. Results from the MTT assay showed WiDr cells was weakly suppressed in the presence of the extract. The result of the assay also showed a very close correlation between the Solanum nigrum extract concentration and the surviving cell numbers which means the extract caused cell death in a dose-dependent fashion in WiDr cancer cells with the IC50 of 359,23 µg/ml. Collectively, the research suggest further studies to explore other chemopreventive possibilites of Solanum nigrum ethanolic extract.

Keywords : colon cancer, MTT assay, cytotoxic, WiDr, Solanum nigrum


Full Text:

PDF

References


ACS, 2007, Global Cancer Facts and Figures 2007, American Cancer Society, Website, Link

Chen, T.R., Drabkowski, D., Hay, R.J., Macy, M. and Peterson, W. Jr., 1987, WiDr is a Derivative of Another Colon Adenocarcinoma Cell Line, HT-29, Cancer Genet. Cytogenet., 27(1), 125-134. Link

Klabunde, C.N., Lanier, D., Breslau, E.S., Zapka, J.G., Fletcher, R.H., Ransohoff, D.F., et al., 2007, Improving Colorectal Cancer Screening in Primary Care Practice: Innovative Strategies And Future Directions, J. Gen Intern. Med., 22(8), 1195-1205. CrossRef

Levrero, M., De Laurenzi, V., Costanzo, A., Gong, J., Wang, J.Y. and Melino, G., 2000, The p53/p63/p73 Family of Transcription Factors: Overlapping and Distinct Functions, J. Cell Sci., 113(10), 1661-1670. Link

Palozza, P., Serini, S., Maggiano, N., Giuseppe, T., Navarra, P., Ranelletti, F.O., et al., 2005, Carotene Downregulates the Steady-State, Heregulin-a-Induced COX-2 Pathways in Colon Cancer Cells, J. Nutr., 135(1), 129-136. CrossRef

Ravi V., Sheikuduman, M.S.T., Patel S.S., Raamamurthy J. and Karunakaran, G., 2009, Anti-Inflammatory Effect of Methanolic Extract of Solanum nigrum Linn Berries, Int. J. Appl. Res. Nat. Prod., 2(2), 33-36. Link

Sigmond, J., Backus, H.H., Wouters, D., Temmink, O.H., Jansen, G. and Peters, G.J., 2003, Induction of Resistance to the Multitargeted Antifolate Pemetrexed (ALIMTA) in WiDr Human Colon Cancer Cells is Associated with Thymidilate Synthase Overexpression, Biochem. Pharmacol., 66(3), 431-438. Link

Son, Y.O., Kim, J., Lim, J.C., Chung, Y., Chung, G.H. and Lee, J.C., 2003, Ripe Fruits Of Solanum Nigrum L. Inhibits Cell Growth and Induces Apoptosis In MCF-7 Cells, Food Chem. Toxicol., 41(10), 1421-1428. Link




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev2iss3pp291-294

Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:

               

               

      

 

Indonesian Society for Cancer Chemoprevention