Combination of Leunca Herb Ethanolic Extract and Doxorubicin Suppresses HeLa Cells’ Growth

Sarmoko Sarmoko; Dyaningtyas Dewi Pamungkas Putri; Endah Puspitasari; Anindyajati Anindyajati; Edy Meiyanto

doi:10.14499/indonesianjcanchemoprev2iss3pp281-285

Combination of Leunca Herb Ethanolic Extract and Doxorubicin Suppresses HeLa Cells’ Growth

Sarmoko Sarmoko, Dyaningtyas Dewi Pamungkas Putri, Endah Puspitasari, Anindyajati Anindyajati, Edy Meiyanto

Abstract

Leunca (Solanum nigrum L.)ethanolic extractshowedcytotoxic activity on several cancer cell lines (HepG2, HT-29) and showed anti-proliferative activityon MCF-7 cells. Its application as a combinationagent in chemotherapy will increase the effectivity and reduce the toxicity of chemotherapy. We predict that application of combinatorial chemotherapy in cancer treatment will be more effective and less toxic compared to single treatment. Our research aims to investigate the cytotoxic activitiy of leunca herbs ethanolic extract alone and in combination with doxorubicin on HeLa cell line. MTT assay was conducted to measure the growth inhibitory effect of leunca herbs ethanolic extract and combinatorial treatments. Leunca herb ethanolic extract (5, 50, 250 μg/ml) increased the cytotoxic effect of doxorubicin compared to doxorubicin alone. The strongest cytotoxic activity resulted from the combination of 250 μg/ml leunca herbs ethanolic extract and 250 nM doxorubicin. Based on our results, leunca herbs ethanolic extract is a potential chemopreventive agent, while its molecular mechanism needs to be explored.

Keywords : Leunca herbs ethanolic extract, doxorubicin, HeLa, MTT assay

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References

Anindyajati, A., Sarmoko, S., Putri, D.D.P., Hermawan, A. and Meiyanto, E., 2010, Combination of Solanumnigrum L., Herb Ethanolic Extract and Doxorubicin Performs Synergism on T47D Breast Cancer Cell Line, Indones. J. Cancer Chemoprevent., 1(2), 78-84. CrossRef

Everist, S.L., 1974, Poisonous Plants of Australia, Angus and Robertson, Sydney.

Hu, K., Kobayashi, H., Dong, A., Jing, Y., Iwasaki, S. and Yao, X., 1999, Antineoplastic Agents III: Steroidal Glycosides from Solanumnigrum, Planta Med., 65(1), 35-38. Link

Istiaji, R.P., Fitria, M., Larasati, Tjondro F., Maruti, A.A., Setyowati, E.P. and Meiyanto, E., 2010, Leunca (Solanumnigrum L.) Herbs Ethanolic Extract Increase Cytotoxic Activity of Cisplatin on Hela Cervical Cancer Cells, Indones. J. Cancer Chemoprevent., 1(1), 32-37. CrossRef

Ji, Y.B.., Gao, S.Y., Ji, C.F. and Zhou, X., 2008, Induction of Apoptosis in HepG2 Cells by Solanine and Bcl-2 Protein, J. Ethnopharmacol, 115(2), 194-202. CrossRef

Kufe, D.W., Pollock, R.E., Weichselbaum, R.R., Bast, R.C., Gansler, T.S., Holland, J.F., et al2003, Holland-Frei Cancer Medicine, 6th Edition, Hamilton: BC Decker.

Liang, C.H., Shiu, L.Y., Chang, L.C., Sheu, H.M., Tsai, E.M. and Kuo, K.W., 2008, Solamargine Enhances HER2 Expression and Increases the Susceptibility of Human Lung Cancer H661 and H69 Cells to Trastuzumab and Epirubicin, Chem. Res. Toxicol., 21(2), 393-399. CrossRef

Liu, L.F., Liang, C.H., Shiu, L.Y., Lin, W.L., Lin, C.C. and Kuo, K.W., 2004, Action of Solamargine on Human Lung Cancer Cells – Enhancement of the Susceptibility of Cancer Cells to TNFs, FEBS Lett., 577(1-2), 67-74. CrossRef

Potter, A.J. and Rabinovitch, P.S., 2005, The Cell Cycle Phases of DNA Damage and Repair Initiated by Topoisomerase II – Targeting Chemotherapeutic Drugs, Mutat. Res., 572(1-2), 27-44. CrossRef

Weller, R.F. and Phipps, R.H., 1979, A Review of the Black Nightshade (Solanumnigrum L.), Protection Ecology, 1, 121-139.

Yang, J., Liu, X., Bhalla, K., Kim, C.N., Ibrado, A.M., Cai, J., et al., 1997, Prevention of Apoptosis by Bcl-2: Release of Cytochrome C from Mitochondria Blocked, Science, 275(5303), 1129-1132. Link