Ficus septica Burm. F. Leaves Ethanolic Extract Induces Apoptosis in 7,12-Dimethylbenz[A]Nthracene-Induced Rat Liver Cancer Quatitavely

Dita Brenna Septhea, Anindyajati Anindyajati, Andita Pra Darma, Ika Nurzijah, Agung Endro Nugroho, Edy Meiyanto

Abstract


The chemopreventive effect of Ficus septica Burm. f. leaves ethanolic extract (FLEE) was studied in 7,12-dimethylbenz[a]nthracene(DMBA)-induced rat liver cancer. Rats were divided into 5 group, 5 rats (5 wk of age Sprague Dawley rat) in each group. Group 1 was control diet group, administered with 0,5% CMC-Na as vehicle. FLEE was administered 750 mg/kgBW and 1500 mg/kgBW starting 4 wk until 5 wk after DMBA administration at the first until fifth wk to group 2 and group 3. Group 4 was control extract group, administered  with 750 mg/kgBW and group 5 was DMBA group. DMBA is a carcinogen to induce liver cancer was also administered in DMBA control group and all animals were necropsied at 6 wk after DMBA administration. Activity of inducing apoptosis was detected using Double Staining method in 750 mg/kgBW FLEE group compared to control group but no in 1500 mg/kgBW FLEE group resulted in 100% dead. Apoptotic cells would have orange flourescence but normal cells would have green flourescence detected by flourescence microscope. To investigate the protein that involved in apoptotic mechanism, we studied p53 expression using Imunohistochemistry (IHC). There was no difference expression of p53 in both tested and control groups. Based on the results, FLEE has a potency as chemoprentive agent because its activity on inducing apoptosis in liver cancer with p53-independent pathway. The mechanism of apoptosis induction of this extract needs to be explored by observing the expression of related proteins.

Keywords: apoptosis, Ficus septica, liver cancer, p53 independent pathway


Full Text:

PDF

References


Davis, J. M., Navolonic, P. M., Weinstein C. R., Steelman, L. S., Hu, Konovlepa, M., et al, 2003, Raf-1 And Bcl-2 Induce Distinct and Common Pathway That Contribute To Cancer Drug Resistance, Clin. Cancer Res., 9(3), 1161-1170. Link

Dempsey, P. W., Doyle, S. E., He, J. Q. and Cheng, G., 2003, The Signaling Adaptors and Pathways Activated By TNF Superfamily, Cytokine Growth Factor Rev., 14(3-4), 193-209. Link

Dolinsky, C., 2011, Breast Cancer: The Basics, Website, http://www.oncolink.org/types/article.cfm?c=3&s=5&ss=33&id=8320&CFID=20529568&CFTOKEN=66709214, accessed on February 5, 2011. Link

Donato, F., Boffetta, P. and Puoti, M. A., 1998, A Meta-Analysis of Epidemiological Studies On The Combined Effect of Hepatitis B And C Virus Infections In Causing Hepatocellular Carcinoma, Int. J. Cancer., 75(3), 347-354. Link

Garcia, M., Jemal, A., Ward, E. M., Center, M. M., Hao, Y., Siegel, R.L., et al., 2007, Global Cancer Facts and Figures 2007, Atlanta, GA: American Cancer Society.

Gerl, R. and Vaux, D. L., 2005, Apoptosis In Development and Treatment of Cancer, Carcinogenesis, 26(2), 263-270. CrossRef

Ghobrial, I.M., Witzig, T.E. and Adjei, A.A., 2005, Targeting Apoptosis Pathways In Cancer Therapy, CA Cancer J. Clin., 55(3), 178-194. Link

Hanahan, D. and Weinberg, R. A., 2000, The Hallmark of Cancer, Cell, 100(1), 57-70. Link

Kaufmann, S.H., and Hengartner, M.O., 2001, Programmed Cell Death: Alive And Well In The New Millennium, Trends Cell Biol., 11(12), 526-534. CrossRef

Mihajlovic, M.L., 2008, Recent Advances in Radiation Therapy of Cancer Cells: A Step towards an Experimental dan Systems Biology Framework, Curr. Radiopharm., 1(1), 22-29. CrossRef

Nurcahya, B.M., 2007, Efek Antiproliferatif Ekstrak Etanolik Daun Awar-Awar (Ficus septica Burm. f.) terhadap Sel Kanker Payudara T47D, Undergrad Thesis, Universitas Gadjah Mada, Departement of Pharmacy, Yogyakarta.

Parkin, D. M., Pisani, P. and Ferlay, J., 1999, Global Cáncer Statistic, CA Cancer J. Clin., 49(1), 33-64. Link

Parkin, D. M., Bray, F., Ferlay, J. and Pisani, P., 2005, Global Cancer Statistics, 2002, CA Cancer J. Clin., 55(2), 74-108. Link

Schuler, M. and Green, D.R., 2001, Mechanism Of P53-Dependent Apoptosis, Biochem, Soc, Trans, 29(6), 684-688. Link

Sekti, D.A., Mubarok, M.F. and Wulandari, A., 2008, Upaya Peningkatan Aktivitas Sitotoksik Doxorubisin pada Sel Kanker Payudara MCF-7 Menggunakan Ekstrak Etanolik Daun Awar–Awar (Ficus Septica Burm. f.) serta Studi Docking Molekuler Alkaloid Fenantroindolisidin sebagai Inhibitor Raf-1, Proceeding, Kongres Ilmiah XVI ISFI 2008. Yogyakarta.

Staerk, D., Lykkeberg, A.K, Christensen, J., Budnik, B.A., Abe, F. and Jaroszewski, J.W., 2002, In Vitro Cytotoxic Activity of Phenanthroindolizidine Alkaloids from Cynanchum vincetoxicum and Tylophora tanakae against Drug-Sensitive and Multidrug Resistant Cancer Cells, J. Nat. Prod., 65(9), 1299-1302. Link

Steele, R. J, Thompson, A. M., Hall P.A. and Lane, D.P., 1998, The P53 Tumor Suppressor Gene, Br. J. Surg., 85(11), 1460-1467. CrossRef

Tamimi, R. M., Lagiou, P., Adami, H. and Trichopoulo, D., 2002, Prospect for Chemopreventionof Cancer, J. Intern. Med., 251(4), 286-300. Link

Tyagi, A. K., Agarwal, C., Chan, D.C. and Agarwal, R. 2004, Synergistic Anti-Cancer Effects of Silibinin With Conventional Cytotoxic Agents Doxorubicin, Cisplatin Dan Carboplatin Against Human Breast Carcinoma MCF-7 Dan MDA-MB468 Cells, Oncol. Rep., 11(2), 493-499. Link




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev2iss2pp255-260

Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:

               

               

      

 

Indonesian Society for Cancer Chemoprevention