Cytotoxic Activity of Cambodian Leaves Extract (Plumeria acuminate) on Breast Cancer Cells and COX-2 Targeted Prediction of Its Chemical Contents
Abstract
Cambodian leaves are suspected to contain stigma sterol which may target Cyclo-Oxygenase-2 (COX-2) or Estrogen Receptor (ER) to contribute to its cytotoxic activity on breast cancer cells. This study aimed to determine the potential of Cambodian leaf compounds and extracts as chemopreventive agents for luminal breast cancer with a molecular target of COX-2. Ethanol was used to extract the active compound of Cambodian leaves. The study on chemical activity against COX-2 employed molecular docking with Molecular Operating Environment (MOE) and the cytotoxic property of Cambodian leaf extract (CLE) on T47D was determined using the trypan blue exclusion method. The extraction yielded as 4.87% w/w CLE. Thin layer chromatography showed that Cambodian leaves contain sterol. Molecular docking confirmed that several sterol compounds have greater affinity to COX-2 than native ligands indicating that they are potent as COX-2 inhibitors. They are Stigmast-7-en-3-ol, Lupeol Acetate, and Lupeol carboxylic acid with docking scores of -14.3874, -13.8098, and -14.1045 kcal/mol respectively. The CLE exhibited cytotoxic activity on T47D cells with an IC50 value of 18 μg/mL. Therefore, CLE has a potential effect as a chemopreventive agent for breast cancer and potentially as a COX-2 inhibitor.
Keywords: Cambodian leaf extract, breast cancer, COX-2 inhibitor, chemopreventive.
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Dhyantari, D. 2015, Efek Antiinflamasi Dari Ekstrak Glukosamin Ceker Ayam Pada Tikus Wistar Jantan Yang Diinduksi Karagenan. The Use Chicken Foot Extraction as the Source of Glucosamine as Anti-Accute Inflamation Agent by In Vivo, Jurnal Pangan dan Agroindustri, 3(3), 888–895. Link
Farooque, Ashraf, M.D., Mazumder, A., Shambhawee, S., and Mazumder, R., 2012, Review on Plumeria Acuminata, International Journal of Research in Pharmacy and Chemistry, 2(2). Link
Gai, W.T., Yu, D.P., Wang, X.S., and Wang, P.T., 2016, Anti-cancer effect of ursolic acid activates apoptosis through ROCK/PTEN mediated mitochondrial translocation of cofilin-1 in prostate cancer, Oncol. Lett., 12(4), 2880-2885. Crossref
Gupta, M., Mazumder, U.K., Gomathi, P., and Selvan, V.T., 2006, Antiinflammatory evaluation of leaves of Plumeria acuminata, BMC Complementary and alternative medicine, 6(1), 1-6. Crossref
Gupta, M., and Yadav, N., 2016, Phytochemical screening of leaves of Plumeria alba and Plumeria acuminat, Journal of Chemical and Pharmaceutical Research, 8(5), 354-358. Link
Harris, Z.M., 2014, Cyclooxygenase-2 and the inflammogenesis of breast cancer, World J Clin Oncol, 5(4), 677-692. Crossref
Harris, R.E., 2009, Cyclooxygenase-2 (COX-2) and the Inflammogenesis of Cancer, Sub-Cellular Biochemistry, 49, 145-165. Crossref
Hidayati, N.A., Listyawati, S., and Setyawan, A.D., 2008, Kandungan Kimia dan Uji Antiinflamasi Ekstrak Etanol Lantana cemara pada tikus putih (Rattus norvegicus L.) Jantan. Thesis. FMIPA. Universitas Sebelas Maret. Surakarta. Link
Hugo, H., Saunders, C., Ramsay, R. and Thompson, E., 2015, New Insights on COX-2 in Chronic Inflammation Driving Breast Cancer Growth and Metastasis, Journal of Mammary Gland Biology and Neoplasia, 20(3-4), 109-119. Crossref
Kementerian Kesehatan RI. 2019. Beban Kanker di Indonesia. Website, https://pusdatin.kemkes.go.id, accessed on July, 20 2021. Link
Patel, D, Jyoti, 2018, Managing Side Effect of Chemotherapy Cancer, accessed on Ocober, 23 2021. Link
Puratchikody, A., Sriram, D., Umamaheswari, A., and Irfan, N., 2016, 3D Structural Interactions and Quantitative Structural Toxicity Studies of Tyrosine Derivatives Intended for Safe Potent Inflammation Treatment, Chemistry Central Journal, 10(24), 1–19. Crossref
Riaz, S., Mehboob, A., and Shahida, H., 2020, Virtual screening of antitumour phytochemical against peroxisome proliferators activated receptor proteins PPARs, Pak. J. Pharm. Sci., 33(1), 379-383. Link
Shofi, M., Suwitasari, F., and Istiqomah, N., 2020, Aktivitas Antioksidan Ekstrak Etanol Kamboja Jepang (Adenium obesum) dan Kamboja Putih (Plumeria acuminata), Al-Kauniyah: Jurnal Biologi, 13(2), 167-178. Crossref
Yu, Z., Xu, W., and Wang, H., 2018, Resveratrol treatment inhibits acute pharyngitis in the mice model through inhibition of PGE2/COX-2 expression, Saudi J. Biol. Sci., 25(7), 1468-1472. Crossref
DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev14iss3pp199-206
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