Correlation Between Antioxidant and Cytotoxic Activity of Parijoto (Medinilla speciosa Blume) Fractions in 4T1 Cell Line

Eva Annisaa', Widyandani Sasikirana, Nuraini Ekawati, Intan Rahmania Eka Dini


Parijoto (Medinilla speciosa Blume) is one of Indonesian plant used for traditional medicine. Previous studies have demonstrated antimicrobial and cytotoxic effects of Parijoto on T47D cells. Therefore, we intended to know the antioxidant and cytotoxic activity of these fractions in 4T1 cell line (a Mus musculus mammary carcinoma). This cancer causes the greatest number of cancer-related deaths This study also investigated the correlation between antioxidant activity and cytotoxicity of Parijoto fractions. Discovering the type of correlation between antioxidant and anticancer activity of botanical extracts could relieve in screening for cytotoxic agent from natural products. The antioxidant and cytotoxic activity investigated using the Diphenylpicrylhydrazyl (DPPH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay methods. The result showed that ethyl acetate fraction is the higher antioxidant activity (IC50:1.77 μg/mL) and the higher cytotoxicity (IC50:133.57 μg/mL). There was a strong positive correlation (correlation coefficient=0.957) between antioxidant and cytotoxic activity in 4T1 cell line, but the correlation was not significant (p=0.188).

Keywords: Parijoto (Medinilla speciosa Blume), antioxidant, cytotoxic, 4T1 cell line.

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Anggana, A.F., 2011, Kajian Etnobotani Masyarakat di Sekitar Taman Nasional Gunung Merapi (Studi Kasus Di Desa Umbulharjo, Sidorejo, Wonodoyo Dan Ngablak), Skripsi, Institut Pertanian Bogor, Bogor.

Artanti, A.N., Pujiastuti, U.H, Prihapsara, F. and Rakhmawati, R., 2020, Synergistic Cytotoxicity Effect by Combination of Methanol Extract of Parijoto Fruit (Medinilla speciosa Reinw. ex. Bl) and Cisplatin against HeLa Cell Line, Indones. J. Cancer Chemoprevent., 11(1), 16-21. CrossRef

Aryal, S., Baniya, M.K., Danekhu, K., Kunwar, P., Gurung, R. and Koirala, N., 2019, Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal, Plants, 8(4), 96. CrossRef

Badan Penelitian dan Pengembangan Kesehatan, 2018, Hasil Utama Riset Kesehatan Dasar 2018, Jakarta: Kementerian Kesehatan RI.

Bennette, L., Rojas, S. and Seefeldt, T., 2012, Role of Antioxidants in the Prevention of Cancer, Journal of Experimental & Clinical Medicine, 4(4), 215-222. CrossRef

DuPré, S.A., Redelman, D. and Hunter, K.W., 2007, The mouse mammary carcinoma 4T1: Characterization of the cellular landscape of primary tumours and metastatic tumour foci, Int J Exp Pathol, 88(5), 351–360. CrossRef

Ferreira, O. and Pinho, S.P., 2012, Solubility of Flavonoids in Pure Solvents, Ind. Eng. Chem. Res., 51(18), 6586–6590. CrossRef

Gomes, C.A., da Cruz, T.G., Andrade, J.L., Milhazes, N., Borges, F., Marques, M. and Paula, M., 2003, Anticancer Activity of Phenolic Acids of Natural or Synthetic Origin: A structure-Activity Study, J.Med.Chem, 46(25), 5395-5401. CrossRef

Hanum, S.A., Prihastanti, E. and Jumari, 2017, Ethnobotany of Utilization, Role, and Philosopical Meaning of Parijoto (Medinilla, spp) on Mount Muria in Kudus Regency, Central Java, Proceeding, Semarang: The 4th International Conference on Research, Implementation, and Education of Mathematics and Science. CrossRef

Ishikawa, K., Takenaga, K., Akimoto, M., Koshikawa, N., Yamaguchi, A., Imanishi, H., Nakada, K., Honma, Y. and Hayashi, J., 2008, ROS-generating mitochondrial DNA mutations can regulate tumor cell metastasis, Science, 320(5876), 661-664. CrossRef

Kim, S.J., Kim, H.S. and Seo, Y.R., 2019, Understanding of ROS-Inducing Strategy in Anticancer Therapy, Oxidative Medicine and Cellular Longevity, 2019(5381692), 1-12. CrossRef

Mumpuni, K.E., Susilo, H. and Rochman, F., 2015, Peran Masyarakat dalam Upaya Konservasi, Proceeding, Surakarta: Seminar Nasional XII Pendidikan Biologi FKIP UNS, pp.779-782.

Niswah, L., 2014, Uji Aktivitas dari Ekstrak Buah Parijoto (Medinilla speciosa Blume) Menggunakan Metode Difusi Cakram, Skripsi, Universitas Islam Negeri Syarif Hidayatullah, Jakarta.

Nordin, M.L., Kadir, A.A., Zakaria, Z.A., Abdullah, R. and Abdulah, M.N.H., 2017, Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1), Evidanced-based Complementary and Alternative Medicine, 2017(9368079), 1-10. CrossRef

Prayong, P., Barusrux, S. and Weerapreeyakul, N., Cytotoxic Activity Screening of Some Indigenous Thai Plants, Fitoterapia, 79(7-8), 598-601. CrossRef

Ruberte, A.C., Ramos-Inza, S., Aydillo, C., Talavera, I., Encío, I., Plano, D. and Sanmartín, C., 2020, Novel N,N′-Disubstituted Acylselenoureas as Potential Antioxidant and Cytotoxic Agents, Antioxidants, 9(1), 55. CrossRef

Sahin, I., Bilir, B., Ali, S., Sahin, K. and Kucuk, O., 2019, Soy Isoflavones in integrative Oncology: Increased Efficacy and Decreased Toxicity of Cancer Therapy, Integr Cancer Ther., 18(1-11), 1-2. CrossRef

Sammar, M., Abu Farich, B., Rayan, I., Falah, M. and Rayan, A., 2019, Correlation between cytotoxicity in cancer cells and free radical scavenging activity: In vitro evaluation of 57 medicinal and edible plant extracts., Oncol. Lett., 18(6), 6563-6571. CrossRef

Simone, C.B.2nd, Simone, N.L., Simone, V. and Simone C.B. 2007, Antioxidants and other nutrients do not interfere with chemotherapy or radiation therapy and can increase kill and increase survival, part 1, Altern. Ther. Health Med, 13(1), 22–28.

Simone, C.B.2nd, Simone, N.L., Simone, V. and Simone, C.B., 2007, Antioxidants and other nutrients do not interfere with chemotherapy or radiation therapy and can increase kill and increase survival, part 2, Altern. Ther. Health Med, 13(2), 40–47.

Singh, K., Bhori, M., Kasu, Y.A., Bhat, G. and Marar, T., 2017, Antioxidants as precision weapons in war against cancer chemotherapy induced toxicity – Exploring the armoury of obscurity, Saudi Pharmaceutical Journal, 26(2018), 177–190. CrossRef

Tao, K., Fang, M., Alroy, J. and Sahagian, G.G., 2008, Imagable 4T1 model for the study of late stage breast cancer, BMC Cancer, 8, 228. CrossRef

Tussanti, I., Johan, A. and Kisdjamiatun, 2014, Sitotoksisitas In Vitro Ekstrak Etanolik Buah Parijoto (Medinilla Speciosa, Reinw.Ex Bl.) Terhadap Sel Kanker Payudara T47D, Jurnal Gizi Indonesia, 2(2), 53-58. CrossRef

Valko, M., Rhodes, C.J., Moncol, J., Izakovic, M and Mazur, M., 2006, Free Radicals, Metals and Antioxidants in Oxidative Stress-Induced Cancer, Chem Biol Interact, 160(1), 1-40. CrossRef

Wang, J and Yi, J., 2008, Cancer Cell Killing Via ROS to Increase or Decrease, That Is The Question, Cancer Biology & Therapy, 7(12), 1875-1884. CrossRef

Widjanarko, M., 2008, Peran Masyarakat Menjaga Kearifan Lingkungan di Kawasan Gunung Muria di Kabupaten Kudus. Thesis. Universitas Katolik Soegijapranata, Semarang.

World Health Organization, 2020, Breast Cancer, Website,, accessed on August 18, 2020.

Ziaei, S. and Halaby, R., 2017, Dietary Isoflavones and Breast Cancer Risk, Medicines (Basel), 4(2), 18. CrossRef


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