Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line

Roihatul Mutiah, Trian Sidha Minggarwati, Risma Aprinda Kristanti, Erna Susanti

Abstract


Eleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find the most active fraction, indicated by its cytotoxic potency on HeLa cervical cancer cell line, and to identify compounds in E. palmifolia bulbs fraction. E. palmifolia bulbs was extracted by maceration. The extraction with ultrasonic bath and partition fractionation was conducted by using n-hexane, chloroform, and ethyl acetate. Each fraction was tested for toxicity level on HeLa cells using MTT assay. The identification of active compounds was carried out by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). The result showed that based on the IC50 value, the ethyl acetate fraction had the highest bioactivity. IC50 values of n-hexane, chloroform, and ethyl acetate fractions were 250.77±19.01; 720.46±42.38; and 44.34±9.45μg/mL, respectively. The identification of the active compound in ethyl acetate fraction resulted 28 chemical compounds. Compounds with the highest percentage area were isoliquiritigenin and oxyresveratrol. The ethyl acetate fraction of E. palmifolia bulbs is potential to be developed as an anticancer candidate (phytopharmaceutical).


Keywords: Compound identification, Anticancer activity, Eleutherine palmifolia (L.) Merr., cervical cancer


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References


Brenton, A.G., and Godfrey, A.R., 2010, Accurate Mass Measurement: Terminology and Treatment of Data, J. Am. Soc. Mass Spectrom, 21(11), 1821-1835.

Chatsumpun, N., Chuanasa, T., Sritularak, B., Lipipun, V., Jongbunprasert, V., Ruchirawat, S., ,i>et al., 2016, Oxyresveratrol: Structural Modification and Evaluation of Biological Activities, Molecules, 21(4), 489. CrossRef

Chung, S.H., Franceshi, S., and Lambert, F., 2010, Estrogenand Erα: Culprits in Cervical Cancer?, Trends Endocrinol Metab., 21(8), 504-511. CrossRef

Doyle, A., and Griffiths, J.B., 2000, Cell and Tissue Culture for Medical Research. New York: John Willey and Sons Ltd.

Galingging, R.Y., 2009, Bawang Dayak (Eleutherine palmifolia) sebagai Tanaman Obat Multifungsi, Warta Penelitian dan Pengembangan, 15(3), 2-4.

Hirchaud, F., Hermetet, F., Ablise, M., Fauconnet, S., Vuitton, D.A., and Prétet, J.L., 2013, Isoliquiritigenin Induces Caspase-Dependent Apoptosis via Downregulation of HPV16 E6 Expression in Cervical Cancer Ca Ski Cells, Planta Med., 79(17), 1628-1635.

Hou, C., Li, W., Li, Z., Gao, J., Chen, Z., Zhao, X., et al., 2017, Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism, Oxid Med Cell Longev, 2017, 1-11. CrossRef

Iwashita, K., Kobori, M., Yamaki, K., and Tsushida, T., 2000, Flavonoids Inhibit Cell Growth and Induce Apoptosis in B16 Melanoma 4A5 Cells, Biosci. Biotechnol. Biochem, 64(9), 1813-1820. CrossRef

Kumar, B., Prakash, A., Ruhela, R.K., and Medhi, B., 2014, Potential of metabolomics in preclinical and clinical drug development, Pharmacol Rep., 66(6), 956-963. CrossRef

Mardiyaningsih, I., 2014, Aktivitas Sitotoksik Ekstrak Etanolik Daun Alpukat (Persea americana Mill.) Pada Sel Kanker Leher Rahim HeLa, Trad Med J., 19(1), 24-28.

Mutiah, R., 2014 Pengembangan Fitofarmaka Antikanker: Panduan dan Teknik Pengembangan Obat Herbal Indonesia Menjadi Fitofarmaka, Malang: UIN-Maliki Press.

Mutiah, R., Listiyana, A., and Suryadinata, A., 2017, Anticancer Activity of Combination of Benalu Belimbing (Macrosolen cochinensis) Extract and Sabrang Onion (Eleutherine palmifolia (L) Merr) on Cervical Cancer Hela Cell Line, Trad Med J., 22(3), 146-152.

Rahmawati, E., Sukardiman, and Muti, A.F., 2013, Aktivitas Antikanker Ekstrak n-Heksana dan Ekstrak Metanol Herba Pacar Air (Impatiens balsamina Linn) terhadap Sel Kanker Payudara T47D, Media Farmasi, 10(2), 2-6. CrossRef

Schuhmacher, R., and Krska, R., 2013, Metabolomics and metabolite profiling, Anal Bioanal Chem., 405,(15), 5003-5004. CrossRef

Skoog, D.A., West, D.M., Holler, F.J., and Crouch, S.R., 2004, Fundamentals of Analytical Chemistry. Eighth Edition. Belmont, CA: Thomson Brooks/Cole.

Suzery, Meiny, and Cahyono, B., 2014, Evaluation of Cytotoxicity Effect of Hyptis pectinata Poit (Lamiaceae) extracts using BSLT and MTT Methods, Jurnal Sains dan Matematika, 22(3), 84-88.

Takahashi, T., Takasuka, N., Ligo, M, Baba, M., Nishino, H., Tsuda, H., et al., 2004, Isoliquiritigenin, a Flavonoid from Licorice, Reduce Prostaglandin E2 and Nitric Oxide, Causes Apoptosis, and Suppress Aberrant Crypt Foci Development, Cancer Sci., 95(5), 448-453. CrossRef

Wang, Z., Wang, N., Han, S., Wang, D., Mo, S., Yu, L., et al., 2013, Dietary Compound Isoliquiritigenin Inhibits Breast Cancer Neoangiogenesis Via VEGF/VEGFR-2 Signaling Pathway, Plos One, 8(7), 1-14. 0068566. CrossRef

Wolfender, J.L., Marti, G., Thomas, A., and Bertrand, S., 2015, Current Approaches and Challenges for the Metabolite Profiling of Complex Natural Extract, J. Chromatogr A., 1382, 136-164. CrossRef

World Health Organization, 2013, WHO Guidance Note: Comprehensive Cervical Cancer Prevention and Control: A Healthier Future for Girls and Women, Geneva: WHO Press.

Yusni, M.A., 2009, Perbedaan Pengaruh Pemberian Fraksi Etanolik Bawang Dayak (Eleutherine palmifolia L. Merr) dengan 5-Fluorourasil Terhadap Penghambatan Pertumbuhan Galur Sel Karsinoma Kolon HT29 Dan Ekspresi p53 Mutan, Thesis, Universitas Sebelas Maret, Surakarta.

Zhao, Y.Y., and Lin, R.C., 2014, UPLC-MS application in disease biomarker discovery: The discovery in proteomics to metabolics, Chem Biol Interact, 215, 7-16. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev10iss3pp131-139

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