Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer Cells

Roihatul Mutiah, Farenza Okta Kirana, Rahmi Annisa, Ana Rahmawati, Ferry Sandra

Abstract


Yellow root (Arcangelisia flava (L.) Merr.) has been scientifically known to have potential as an antimalarial, antibacterial, antioxidant, and anticancer. The purpose of this study was to determine the profile of alkaloid content and cytotoxicity of yellow root extract from several regions in Kalimantan. The alkaloid content was tested using the thin layer chromatography (TLC) method with dragendorf reagent. Cytotoxic in vitro test was conducted against WiDr colorectal cancer cells using the 3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide (MTT) assay. Yellow roots were collected from Samarinda city, Banjarmasin city, Barito Timur regency, Malinau district, and Balikpapan City. The MTT inhibitory concentration 50 (IC50) of yellow root extracts were 573.308 μg/mL; 582.857 μg/mL; 296.326 μg/mL; 114.119 μg/mL; and 320.162 μg/mL respectively. Results of the compound identification indicated that alkaloid was found in A. flava from all regions. Alkaloids of A. flava extract should be investigated further in order to find possible active agent that could decrease the viability of WiDr colorectal cancer cells.


Keywords: Arcangelisia flava, Borneo, colorectal cancer, Kalimantan, WiDr cells.


Full Text:

PDF

References


Arung, E.T., Wicaksono, B.D., Handoko, Y.A., Kusuma, I.W., Shimizu, K., Yulia, D., et al., 2010, Cytotoxic effect of artocarpin on T47D cells, J. Nat. Med., 64(4), 423-429. CrossRef

Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R.L., Torre, L.A. and Jemal, A., 2018, Global Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries, C.A. Cancer J. Clin., 68, 394-424. CrossRef

Hendarmin, L., Kawano, S., Yoshiga, D., Sandra, F., Mitsuyasu, T., Nakao, Y., et al., 2008, An Anti-apoptotic Role of NF-κB in TNFα-induced Apoptosis in an Ameloblastoma Cell Line, Oral Sci. Int., 5, 96-103.

Heyne, K., 1987, Tumbuhan Berguna Indonesia. Volume 4, Jakarta: Yayasan Sarana Wana Jaya.

Keawpradub, N., Dej-adisai, S. and Yuenyongsawad, S., 2005, Antioxidant and cytotoxic activities of Thai medicinal plants named Khaminkhruea: Arcangelisia flava, Coscinium blumeanum and Fibraurea tinctoria, Songklanakarin J. Sci. Technol., 27(Suppl. 2), 455-467.

Kim, T. and Croce, C.M., 2018, Long noncoding RNAs: Undeciphered Cellular Codes Encrypting Keys of Colorectal Cancer Pathogenesis, Cancer Lett., 417, 89-95. CrossRef

Lovin, E.R., Arwati, H. and Ramadhani R.B., 2012, In vitro, Intraerythrocytic Antimalaria Activity of Akar Kuning (Arcangelisia flava (L) Merr) Stem Aqueous Extract in Plasmodium falciparum, Folia Medica Indonesiana, 48(3), 90-95.

Maryani, Marsoedi, Nusryam, H. and Maftuch, 2013, The Phytochemistry and The Anti-bacterial Activity of Yellow Root (Arcangelisia flava Merr.) Against Aeromonas hydrophyla, J. Bio. Life Sci., 4(2), 180-190. CrossRef

Minarno, E.B., 2015, Skrining Fitokimia dan Kandungan Total Flavanoid Pada Buah Carica pubescens Lenne & K. Koch di Kawasan Bromo, Cangar, Dan Dataran Tinggi Dieng, El-Hayah, 5(2), 73-82. CrossRef

Mpapa, B.L., 2016, Analisis Kesuburan Tanah Tempat Tumbuh Pohon Jati (Tectona grandis L.) pada Ketinggian yang Berbeda, Jurnal Agrista, 20(3), 135-139. Link

Pratama, M.R.F., 2016, Akar Kuning (Arcangelisia flava) Sebagai Inhibitor EGFR: Kajian in silico, Farmagazine, 3(1), 6-17. Link

Rahmadania, E., Wibowo, A.A. and Rosida, L., 2016, Distribusi Pola Diet Pasien Kanker Kolorektal Di RSUD Ulin Banjarmasin Periode Agustus-Oktober 2015, Berkala Kedokteran, 12(2), 215-227.

Sandra, F., Harada, H., Nakamura, N. and Ohishi, M., 2004, Midkine induced growth of ameloblastoma through MAPK and Akt pathways, Oral Oncol., 40(3), 274-280. CrossRef

Sandra, F. and Rita Lahirin, R., 2017, Human Umbilical Cord Blood Serum Has Higher Potential in Inducing Proliferation of Fibroblast than Fetal Bovine Serum, Mol. Cell. Biomed. Sci., 1(2), 65-69. CrossRef

Sandra, F. and Sidharta, M.A., 2017, Caffeic Acid Induced Apoptosis in MG63 Osteosarcoma Cells Through Activation of Caspases, Mol. Cell. Biomed. Sci., 1(1), 28-33. CrossRef

Sandra, F., 2018, Survivin Ser81 Plays An Important Role in PI3K/Akt/mTOR Signaling Pathway, Mol. Cell. Biomed. Sci., 2(2), 55-59. CrossRef

Tatuhey, W.S., Nikijuluw, H. and Mainase, J., 2014, Karakteristik Kanker Kolorektal Di RSUD Dr. M Haulussy Ambon Periode Januari 2012-Juni 2013, Molucca Medica, 4(2), 150-157.

Vallianou, N.G., Evangelopoulos, A., Schizas, N. and Kazazis, C., 2015, Potential anticancer properties and mechanisms of action of curcumin, Anticancer Res., 35(2), 645-651. Link

Wang, K., Zhang, C., Bao, J., Jia, X., Liang, Y., Wang, X., et al., 2016, Synergistic Chemopreventive Effect of Curcumin and Berberine on Human Breast Cancer Cell Through Induction of Apoptosis and Autophagic Cell Death, Sci. Rep., 6, 1-14. CrossRef

Widowati, W., Mozef, T., Risdian, C., Ratnawati, H., Tjahyani, S. and Sandra, F., 2010, Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line, Indones. J. Cancer Chemoprevent., 1(2), 99-107.

Widyatmoko, D. and Zick., F., 1998, The Flora of Bukit Tiga Puluh National Park, Kerumutan Sanctuary and Mahato Protective Reserve, Riau, Indonesia. Bogor: Indonesian Botanical Gardens in collaboration with Yayasan Sosial Chevron dan Texaco Indonesia.




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev11iss2pp84-89

Copyright (c) 2020 Indonesian Journal of Cancer Chemoprevention

Indexed by:

                  

               

 

Indonesian Society for Cancer Chemoprevention