Cytotoxicity Assay From Fractions of Hedyotis corymbosa Extract Against Breast Cancer Cell Line T47D

Rina Andriyani, Chandra Risdian, Zalinar Udin

Abstract


Drug discovery for cancer medication is the most important effort that researcher do at this time. Indonesia bio diversities have possibility as a cancer medicine sources. Finding a herbal medicine for cancer treatment is a first step to find a right cancer medicine in the future. This research has already completed for the earlier another research. Some fractions of Hedyotis corymbosa extract has been analyzed using Sulforhodamine B method with UV wavelength 515 nm against T47D cell line, a human breast cancer. There are Hexane extract, Methylene chloride extract and Ethyl acetate extract, and give inhibitory concentration 50 (IC50)  of 33.45 µg/ml, 54.59 µg/ml and 52.58 µg/ml, respectively. Ethanolic extract, itself has IC50 of 61.57 µg/ml, whereas IC50 value of Cisplatinum is  9.63 µg/ml. There is a difference between the ethanolic extracts with the other fraction.

Keywords: breast cancer, herbal medicine, T47D, Hedyotis corymbosa

Full Text:

PDF

References


Ahmad, R., Ali, A.M., Israf, D.A., Ismail, N.H., Saari, K. and Lajis, N.H., 2004, Antioxidant, Radical Scavenging, Anti-Inflamatory, Cytotoxic And Antibacterial Activities Of Methanolic Extracts Of Some Hedyotis Species, Life Sci., 76(17), 1953-1964. CrossRef

Jiang, W., Kuang, L.S., Hou, A.J., Qian, M. and Li, J.Z., 2007, Iridoid glycosides from Hedyotis corymbosa, Helv. Chim. Acta., 90(7), 1296-1301. CrossRef

Liou, W,C., Lin, Y,C., Y,M. and Chen, F,C., 1979, Chemistry (Taipei) 72.

Sadasivan, S., Latha, P.G., Sasikumar, J.M., Rajashekaran, S., Shyamal, S. and Shine, V.J., 2006, Hepatoprotective Studies on Hedyotis corymbosa (L.) Lam., J. Ethnopharmacol., 106(2), 245-249. CrossRef

Ho, T.I., Chen, G.P., Lin, Y.C., Lin, Y.M. and Chen, F.C., 1986, An anthraquinone from Hedyotis diffusa, Phytochemistry., 25(8), 1988-1989. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev2iss1pp182-186

Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:

               

               

      

 

Indonesian Society for Cancer Chemoprevention