Cytotoxic Potential of Flavonoid from Nicotiana tabacum Leaves on MCF-7 Human Breast Cancer Cells
Abstract
Flavonoid has potential bioactivity as anticancer agents. The flavonoid of cultivated tobacco (Nicotiana tabacum), locally known as “Kasturi”, leaves was screened for its cytotoxicity against MCF-7 human breast cancer cells and non-transformed Vero cells (African normal cell kidney line) in different concentrations. This study aimed to examine the cytotoxic potential of the flavonoid of Kasturi tobacco leaves against MCF-7 human breast cancer cells. Flavonoid obtained from methanolic extracts of Kasturi tobacco leaves, which have been purified from nicotine. The flavonoid of Kasturi tobacco leaves with concentrations of 20 to 640 μg/mL were exposed to MCF-7 and Vero cells for 24 h. Cell viability was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Flavonoid of Kasturi tobacco leaves with concentrations of 160 μg/mL decreased the MCF-7 cell viability more than 50%, with an inhibitory concentration 50 (IC50) value of 148.41 μg/mL. Meanwhile, it inhibited 50% of Vero cell viability at 255.35 μg/mL. The flavonoid of Kasturi tobacco leaves has cytotoxic activity on MCF-7 cells, and might be a potential alternative agent for human breast cancer therapy.
Keywords: flavonoid, tobacco leaves, human breast cancer cells, anticancer activity
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Brusselmans, K., Vrolix, R., Verhoeven, G. and Swin¬nen, J.V., 2002, Induction of cancer cell apopto¬sis by flavonoids is associated with their ability to inhibit fatty acid synthase activity, J. Biol. Chem., 280(7), 5636–5645. CrossRef
Danciu, C., Vlaia, L., Fetea, F., Hancianu, M., Cori¬covac, D.E., et al., 2015, Evaluation of phenolic profile, antioxidant and anticancer potential of two main representants of Zingiberaceae family against B164A5 murine melanoma cells, Biol. Res., 48, 1. CrossRef
Docheva, M., Dagnon, S. and Statkova-Abeghe, S., 2014, Flavonoid content and radical scavenging potential of extracts prepared from tobacco culvitars and waste, Nat. Prod. Res., 28(17), 1328-1334. CrossRef
Du, G., Lin, H., Wang, M., Zhang, S., Wu, X., et al., 2010, Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1 a in tumor and normal cells, Cancer Chemother. Pharmacol, 65(2), 277–287. CrossRef
Fathiazad, F., Delazar, A., Amiri, R. and Sarker, S.D., 2006, Extraction of flavonoids and quantifica¬tion of rutin from waste tobacco leaves, IJPR., 5(3), 222-227. CrossRef
Gibellini, L., Pinti, M., Nasi, M., De Biasi, S., Roat, E., et al., 2010, Interfering with ROS metabolism in cancer cells: the potential role of quercetin, Cancers, 2(2), 1288–1311. CrossRef
Hashemzaei, M., Far, A.D., Yari, A., Heravi, R.E., Tabrizian, K., et al., 2017, Anticancer and apoptosis-inducing effects of quercetin in vitro and in vivo, Oncol. Rep., 38(2), 819–828. CrossRef
Hossain, M. and Salehuddin, S., 2013, Analytical determination of nicotine in tobacco leaves by gas chromatography–mass spectrometry, Arabian Journal of Chemistry, 6, 275-278. CrossRef
Karabegović, I.T., Veljković, V.B. and Lazić M.L., 2011, Ultrasound-assisted extraction of total phenols and flavonoids from dry tobacco (Nicotiana tabacum) leaves, Nat. Prod. Commun., 6(12), 1855-1856. CrossRef
Kenny, P.A., Lee, G.Y., Myers, C.A., Neve, R.M., Semeiks, J.R., et al., 2007, The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression, Mol. Oncol., 1(1), 84–96. CrossRef
Lacroix, M., Toillon, R.A. and Leclercq, G, 2006, p53 and breast cancer, an update. Endocr. Relat. Cancer, 13(2), 293–325. CrossRef
Le Marchand, L., 2002, Cancer preventive effects of flavonoids a review, Biomed. Pharmacother., 56(6), 296–301. CrossRef
Metwaly, H.A., Al-Gayyar, M.M., Eletreby, S., Ebrahim, M.A. and El-Shishtawy, M.M., 2012, Relevance of serum levels of interleukin-6 and syndecan-1 in patients with hepatocellular carcinoma, Sci. Pharm., 80(1), 179-188. CrossRef
Mujwah, A., Mohammed, M. and Ahmed, M., 2010. First isolation of a flavonoid fom Juniperus procera using ethyl acetate extract, Arabian Journal of Chemistry, 3, 85-88. CrossRef
Peters, G.J., Backus, H.H., Freemantle, S., van Triest, B., Codacci-Pisanelli, G., et al., 2002, Induction of thymidylate synthase as a 5-flu¬orouracil resistance mechanism, Biochim. Bio¬phys. Acta., 1587(2-3), 194-205. CrossRef
Srivastava, S., Somasagara, R.R., Hegde, M., Nishana, M., Tadi, S.K., et al., 2016, Quercetin, a natural flavonoid interacts with dna, arrests cell cycle and causes tumor regression by activating mitochondrial pathway of apoptosis, Sci. Rep., 6, 24049. CrossRef
Stankovic, M., 2011, Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L, Extracts, 33, 63-72.
Tsao, A.S., Edward, S.K. and Hong, W.K., 2004, Chemoprevention of cancer, C.A. Cancer J. Clin., 54(3), 150–180. CrossRef
Wang, X., Yuan, S., Wang, J., Lin, P., Liu, G., et al., 2006, Anticancer activity of litchi fruit pericarp extract against human breast cancer in vitro and in vivo, Toxicol. Appl. Pharmacol., 215(2), 168-178. CrossRef
Xie, F., Yu, A., Hou, D., Liu, H., Ding, L., et al., 2011, Rapid and sensitive analysis of eight polyphenols in tobacco by rapid resolution liquid chro¬matography, AJAC, 2(2), 929-933. CrossRef
Yang, C.S., Landau, J.M., Huang, M.T. and Newmark, H.L., 2001, Inhibition of carcinogenesis by dietary polyphenolic compounds, Annu. Rev. Nutr., 21, 381–406. CrossRef
DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev11iss2pp97-10
Copyright (c) 2020 Banun Kusumawardani
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Indonesian Society for Cancer Chemoprevention
