A New Compound (8,9) -Furanyl-Pterocarpan-3-Ol Used for Standardization of Bengkuang (Pachyrhizus erosus) Extract as Sunscreen and Skin Whitening Agent

Endang Lukitaningsih, Ulrike Holzgrabe

Abstract


Bengkuang (Pachyrhizus erosus) has been traditionally used as sun screening and skin whitening. The active compounds in bengkuang extract already published included their activities in antioxidant and skin whitening. However, standardization of bengkuang extract has not been studied. This research aims to find out the analysis procedure by High Performance Liquid Chromatography to make standardization bengkuang extract.

The first step of this research was collecting bengkuang from Prembun, Central Java, Indonesia in dry season. After cleaning and peeling, bengkuang root was sliced, dried and ground to make powder. Then followed by extraction using Soxhlet in petroleum ether and subsequently in methanol. Methanol extract was evaporated and then partitioned with ethyl acetate-water. Ethyl acetate fraction was evaporated and then separated in open column chromatography using silica gel as stationary phase and a gradient mixture of chloroform-ethyl acetate-methanol as mobile phase. Bio guided fraction method was used for separation and purification to get isolated compounds. The isolated compounds obtained from this fractionation were then elucidated and analyzed their activities.

A new compound (8,9-furanyl-pterocarpan-ol) has been selected as a biomarker for extract standardization. The optimum of HPLC condition for standardization consisted of a column (Zorbax SB-C18; i.d. 0.46 cm; 5 μm particle size), mobile phase (gradient elution of MeOH-water) with flow rate of 1 ml/min and detector (UV-detector at 293 nm). The obtained LOD value was 0.51 ± 0.02 µg. The potentials of this compound to absorb UV ray, antioxidant and anti-tyrosinase were 4.018 mAU*S/mml; 2.113±0.001mM (SC50); 7.19±0.11 mM (IC50), respectively.

Keywords : bengkuang (Pachyrhizus erosus) extract, (8,9)-furanyl-pterocarpan-3-ol, standardization, sunscreen, skin whitening


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References


Boyera, N., Galey, I. and Bernard, B.A., 1998, Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblast, Int. J. Cosmet. Sci., 20(3), 151-158. CrossRef

Chan, C.C., Lam, H., Lee, Y.C. and Zang, X.M., 2004, Analytical Method Validation and Instrument Performance Verification, John Wiley & Sons Inc., Canada, 16–24. Link

Chen, Q. and Kubo, I., 2002, Kinetics of mushroom tyrosinase inhibition by quercetin, J. Agric. Food Chem., 50, 4108-4112. CrossRef

D’Aniello, C., Cermola, F., Patriarca, E.J. and Minchiotti, G., 2017, Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics, Stem Cells Internat., 2017, Hindawi, 1-16. CrossRef

El-Zawawy, N.A. and Ali, S.S., 2016, Pyocyanin as anti-tyrosinase and anti tinea corporis: A novel treatment study, Microb. Pathogen., 100, 213–220. CrossRef

Giampieri, F., Alvarez-Suarez, J., Mazzoni, L., Forbes-Hernandez, T., Gasparrini, M., Gonzàlez-Paramàs, A., et al., 2014, Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality, Molecules, 19, 7798–7816. CrossRef

Gülçin, İ., 2012, Antioxidant activity of food constituents: an overview, Archiv. Toxicol., 86, 345–391. CrossRef

Hearing, V.J.Jr., 1987, In: Methods in Enzymology, Academic Press, New York, 142, 154-165.

Tobin, D.J., 2017, Introduction to skin aging, J. Tissue Viability, 26(1), 37-46. CrossRef

Karim, A.A., Azlan, A., Ismail, A., Hashim, P., Gani, S. salwa abd, and Zainudin, B.H., et al., 2014. Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract, BMC Complement. Alternat. Med., 14, 381. CrossRef

Kedare, S.B. and Singh, R.P., 2011, Genesis and development of DPPH method of antioxidant assay, J. Food Sci. Technol., 48, 412– 422. CrossRef

Kim, Y.H., Kim, K.H., Han, C.S., Yang, H.C., Park, S.H. and Jang, H.-I., et al., 2010, Anti-wrinkle activity of Platycarya strobilacea extract and its application as a cosmeceutical ingredient, J. Cosm. Sci., 61, 211. Link

Lukitaningsih, E., Bahi, M. and Holzgrabe, U., 2013, Tyrosinase Inhibition Type of Isolated Compounds Obtained from Pachyrhizus erosus, Aceh Int. J. Sci. Technol., 2(3), 98-102. CrossRef

Lukitaningsih, E. and Holzgrabe, U., 2014, Bioactive Compounds in Bengkoang (Pachyrhizus erosus) as Antioxidant and Tyrosinase Inhibition Agents, Indonesian J. Pharm., 25(2), 68-75. CrossRef

Manela-Azulay, M. and Bagatin, E., 2009, Cosmeceuticals vitamins, Clin. Dermatol, 27, 469-474. CrossRef

Menaa, F., Menaa, A. and Tréton, J., 2014, Polyphenols against Skin Aging in: Polyphenols in Human Health and Disease, Elsevier, 819–830. CrossRef

Nurrochmad, A., Leviana F., Wulancarsari, C.G. and Lukitaningsih, E., 2010, Phytoestrogens of Pachyrhizus erosus prevent Bone Loss in an Ovariectomized Rat Model of Osteoporosis, Int. J. Phytomed, 2, 363-372. CrossRef

Nurrochmad, A., Lukitaningsih, E., Monikawati, A., Septhea, D.B. and Meiyanto., E., 2013, Combination of low-concentration of novel phytoestrogen (8,9)-furanyl-pterocarpan-3-ol from Pachyrhizus erosus attenuated tamoxifen-associated growth inhibition on breast cancer T47D cells, Asian Pac. J. Trop. Biomed., 3(11), 847-852. CrossRef

Ochiai, A., Tanaka, S., Tanaka, T. and Taniguchi, M., 2016, Rice Bran Protein as a Potent Source of Antimelanogenic Peptides with Tyrosinase Inhibitory Activity, J. Nat. Prod., 79, 2545–2551. CrossRef

Pandey, P. and Tripathi, S., 2014, Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug, J. Pharmacog. Phytocem., 2(5), 115-119. CrossRef

Poljšak, B., Dahmane, R.G. and Godić, A., 2012, Intrinsic skin aging: the role of oxidative stress, Acta Dermatovenerol Alp Pannonica Adriat, 21, 33–6. CrossRef

Rangkadilok, N., Sitthimonchai, S., Worasuttayangkurn, L., Mahidol, C., Ruchirawat, M. and Satayavivad, J., 2006, Evaluation of free radical scavenging and antityrosinase activities of standardized longan fruit extract, Food Chem. Toxicol., 1016-1024. CrossRef

Sharma, O.P. and Bhat, J.K., 2009, DPPH antioxidant assay revisited, Food. Chem., 113(4), 1202-1205. CrossRef

Tran, D., Townley, J.P., Barnes, T.B. and Greive, K.A., 2015, An antiaging skin care system containing alpha hydroxy acids and vitamins improves the biomechanical parameters of facial skin, Clin. Cosm. Invest. Dermatol, 8, 9-17. CrossRef

Widyatmoko, A., Hastutik, D., Sudarmanto, A. and Lukitaningsih, E., 2016, Vitamin C, Vitamin A and Alpha Hydroxy Acid in Bengoang (Pachyrhizus erosus), Trad. Med. J., 21(1), 48-54. CrossRef

Zhang, J., 2013, Antioxidants and Aging, in: Bioactive Food as Dietary Interventions for the Aging Population, Elsevier, 241–248. CrossRef

Zhu, Q., Nakagawa, T., Kishikawa, A., Ohnuki, K. and Shimizu, K., 2015, In vitro bioactivities and phytochemical profile of various parts of the strawberry (Fragaria × ananassa var. Amaou), J. Function. Foods, 13, 38–49. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev10iss2pp60-70

Copyright (c) 2019 Endang Lukitaningsih, Ulrike Holzgrabe

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