Exposure of Murattal Al-Quran Audio Enhances Cisplatin Activity on Growth Inhibition and Cell Cycle Modulation on Hela Cells

Roihatul Mutiah, Muhammad Ragib Mustofa, Yen Yen Ari Indrawijaya, Abdul Hakim, Rahmi Annisa, Nurlaili Susanti, Ach Nashichuddin, Muhammad Zainuddin

Abstract


Cancer is a disease characterized by abnormal cell mechanisms. The development of alternative cancer treatments is still needed. One of them is the music therapy. The music therapy uses sound vibrations to improve healing. Al-Quran, the Holy book of muslims, recites with measured recitation produces beautiful tones. Reading and recitating of Al-Quran is an important form of worship for which a Muslim can expect reward and benefit in the Hereafter. Al-Fatihah is one of surahs in Al-Qur'an that is often read by Muslims and used as a prayer for healing. The purpose of this study was to determine the effect of cytotoxic activity and cell cycle modulation on Hela cells with exposure of murattal Al-Quran and cisplatin combination. Audio exposure murattal Al-Fatihah and its combination with cisplatin to HeLa cells were tested using the MTT method assay. Induction of apoptosis and modulation of cell cycle evaluated by flow cytometry method. Treatment used 30 minutes Audio Murattal (AM), Cisplatin 10 µg/mL (Cis), and the combination of AM + Cis caused a decrease in the viability of HeLa cells respectively 80.14%, 69.86%, and 64.32%. The results of flow cytometry explained that in treatment of AM there was inhibition in the G2-M phase and induction of apoptosis in the M5 phase. Whereas in treatment AM + Cis inhibition occurs in the S, G2-M phase, and induction of apoptosis in the M5 phase. Audio Murattal Al-Quran presents cytotoxic effects on HeLa cells and to provide a synergistic impact on cisplatin so that disclosure therapy murattal can be recommended for supporting therapy in the treatment of cancer (supportive therapy).

Keywords: Al-Quran, Audio murottal, HeLa Cells, Cell Viability, Flow cytometry


Full Text:

PDF

References


Al-Kaheel, A.D., 2012, Treatment of Qurani, Efficacious treatment with Al-Quran. [Translation] ‘Alij Nafsaka bi Al-Quran. Translated by: Muhammad Misbah. Jakarta: AMZAH.

Al-Qurthubi, 2006, Al-Jami’ li Ahkamil Qur’an. 1st ed. Al Resalah Publishers. Beirut. 110-111.

Da’i, M., Supardjan, A.M., Jenie, U.A., Kawaichi, M. and Meiyanto, E., 2011, Pentagamavunon-1 inhibition T47D cell cycle caspase induced inhibitor Z-VAD-Fmk in G2-M phase, Indonesian Journal of Pharmacy, 5, 180-187. Link

DeFilippis, R.A., Goodwin, E.C., Wu, L. and DiMaio, D., 2003, Endogenous Human Papillomavirus E6 and E7 Proteins Differentially Regulate Proliferation, Senescence, and Apoptosis in HeLa Cervical Carcinoma Cells, Journal of Virology, 77, 1551-1563. CrossRef

Dharmayanti, N.L.P., 2003, Study of Molecular Biology: Tumor suppressor gen (p53) as target gen in cancer treatment, Wartazoa, 13, 99-107. CrossRef

Djohan, 2006, Music therapy, theory and application. Yogyakarta: Galangpress.

Goodwin, E.C. and DiMaio, D., 2000, Repression of Human Papilloma Virus Oncogenes in HeLa Cervical Carcinoma Cells Causes the Orderly Reactivation of Dormant Tumor Suppressor Pathways, Biochemistry, 97, 12513-12518. CrossRef

Handayani, S., Susidarti, R.A., Jenie, R.I. and Meiyanto, E., 2017, Two Active Compounds from Caesalpinia sappan L. in Combination with Cisplatin Synergistically Induce Apoptosis and Cell Cycle Arrest on WiDr Cells, Advanced Pharmaceutical Bulletin, 7, 375-380. CrossRef

Heather, S., 2007, What is Sound Healing?, The International Journal of Healing and Caring, 7, 1-11. Link

Hondermarck, H., 2003, Breast Cancer, When Proteomics Challenges Biological Complexity, Molecular & Cellular Proteomics 2.5, 2, 281-291. CrossRef

Ibrahim, M.A., Shah, M.S.M. and Mohd, R.A., 2017, Concept of shifa in Al-Quran: Quranic medicine approach in healing physical ailment, 2nd International Conference on Islam, Science & Education: University Sains Malaysia. Link

Ismiyati, N. and Nurhaeni, F., 2016, Basil leaf extract effect (Ocimum sanctum L.) as chemopreventive agent in Hela cervical cancer cells through cytotoxic activity and apoptosis induction, Media Farmasi, 13, 35-48. CrossRef

Istindiah, H.N. and Auerkari, E.I., 2001, Cell cycle control mechanism: a special review of the protein regulator’s role in retinoblastoma pathways (Rb), JKGUI, 8, 39-47.

Jones, H., Feth, L., Rumpf, D., Hefti, A. and Mariotti, A., 2000, Acoustic Energy Affects Human Gingival Fibroblast Proliferation but Leaves Protein Production Unchanged, J Clin Periodontol, 27, 832-838. CrossRef

Khatoni, A., 1997, The Effect of Reciting the Quran on Anxiety of Patients Hospitalized in the Cardiac Intensive Care Unit of the Selected hospitals in Tehran: Master Thesis, Tehran, J Iran Univ Med Sci., 39, 4-22.

Larasati, Y.A., Putri, D.D.P., Utomo, R.Y., Hermawan, A. and Meiyanto, E., 2014, Combination of Cisplatin and Cinnamon Essential Oil Inhibits HeLa Cells Proliferation Through Cell Cycle Arrest, Journal of Applied Pharmaceutical Science, 4, 014-019. CrossRef

Lestard, N.R., Valente, R.C., Lopes, A.G. and Capella, M.A.M., 2013, Direct Effects of Music in Non-Auditory Cells in Culture, Noise and Health, A Bimonthly Inter-Disciplinary International Journal, 15, 307-314. CrossRef

Lestard, N.R. and Capella, M.A.M., 2016, Exposure to Music Alters Cell Viability and Cell Motility of Human Nonauditory Cells in Culture, Evidence-Based Complementary and Alternative Medicine, 2016, 1-7. CrossRef

Maman, F., 1997, The Role of Music in the 21st Century – Book 1. Redondo Beach, CA: Tama-Do Press.

Mehrafsar, A. and Mokhtari, M.J., 2018, Effect of exposure to Quran recitation on cell viability, cell migration, and BCL2L12 gene expression of human prostate adenocarcinoma cell line in culture, Health, Spirituality and Medical Ethics, 5(4), 46-52. CrossRef

Murti, H., Boediono, A., Setiawan, B. and Sandra, F., 2007, Cell cycle regulation: key success somatic cell nuclear transfer, CDK, 34,, 312-316. Link

Mustamir, 2008, 5 Healing methods of the heavens. Yogyakarta: Lingkaran.

Nemoto, Y., 2014, Message from Water and Science. The 9th Annual Conference on the Physics, Chemistry, and Biology of Water; Bulgaria, 2014. CrossRef

Nisyak, K. and Farid, R.M., 2017, Antioxidant and cytotoxic activity of methanol bark extract Cambodian stem (Plumeria acuminate) against cervical cancer cells, Jurnal Tumbuhan Obat Indonesia, 10, 37-44. Link

Oemiati, R., Rahajeng, E. and Kristanto, A.Y., 2011, The prevalence of tumors and some factors that affect them in Indonesia, Buletin Penelitian Kesehatan, 39, 190-204. CrossRef

Prabasari, C.I.W. and Budiana, I.N.G., 2017, Profile of cervical cancer in general Hospital Sanglah Denpasar, Bali period July 2012-June 2013, E-Jurnal Medika, 6, 1-9.

Putri, Y.K. and Rusdiana, T., 2016, Comparison of various drug interactions with herbs: Article Review, Farmaka Suplemen, 14, 203-214. CrossRef

Sayyid, S.A., 2008, The Secret of Al-Fatihah & Ayat Kursi. Solo: Mumtaza.

Sundarajan, S. and Vogelzang, N., 2014, Chemotherapy in the treatment of prostate cancer – the past, the present, and the future, American Journal of Hematology and Oncology, 10(6), 14-21. Link

Yudhani, R.D., Pesik, N.R. and Indarto, D., 2016, Metformin Enhances Anti-Proliferative Effect of Cisplatin in Cervical Cancer Cell Line, Indonesian Journal of Clinical Pharmacy, 5, 75-83. CrossRef

Zhang, M., Marcela, C., Yang, E.B., Wong, K.P. and Mack, P., 2001, Modulation of cisplatin cytotoxicity and cisplatin-induced DNA cross-links in HepG2 cells by regulation of glutathione-related mechanism, Molecular Pharmacology, 59, 837-843. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev10iss2pp71-79

Copyright (c) 2019 Roihatul Mutiah, Muhammad Ragib Mustofa, Yen Yen Ari Indrawijaya, Abdul Hakim, Rahmi Annisa, Nurlaili Susanti, Ach Nashichuddin, Muhammad Zainuddin

Indexed by:

               

               

      

 

Indonesian Society for Cancer Chemoprevention