Antioxidant Properties and Cytotoxic Activity of Ethyl Acetate Fraction of Plectranthus amboinicus (Lour.) Spreng. Leaves on HeLa and T47D Cell Lines
Abstract
Research into plants with anticancer effects is actively encouraged in orderto discover new drugs with lessertoxicity but more potent effects. The aims of study are to evaluate the antioxidant properties and to investigate the cytotoxic activity of Plectranthus amboinicus (Lour.) Spreng. leaves ethyl acetate fractions on HeLa,T47D and MCF7 cell lines. The extract was prepared by graded maceration using n-hexane and ethyl acetate. The ethyl acetate extract was fractionated in vacuum liquid chromatography with n-hexane: ethyl acetate; and ethyl acetate: methanol as mobile phase. Then, the fractions were analyzed with thin layer chromatography (TLC). The free radical scavenging activity was measured by DPPH method, the total flavonoid content was calculated by quercetin equivalent and the absorbance is measured by using UV-Visible spectrophotometry. The cytotoxic activity were determined using MTT assay. The fractions contained 5 sub fractions with same TLC profile. The fractions showed antioxidant activity by DPPH method with different IC50 values, namely: 130 µg/mL(I), 127 µg/mL(II), 137 µg/mL(III), 129 µg/mL(IV), and 124 µg/ mL(V), respectively. The measurement of total flavonoid content showed 118 mg QE/g (I), 50 mg QE/g (II), 207 mg QE/g (III), 56 mg QE/g (IV), and 55 mg QE/g (V). The IC50 of each sub fractions on HeLa cell were 77 µg/mL, 46 µg/mL, 93 µg/mL, 71 µg/mL and 476 µg/mL; for T47D cell were 1621 µg/mL, 111 µg/mL, 128 µg/mL, 150 µg/mL and 209 µg/mL; and for MCF7 were 259 µg/mL, 343 µg/mL, 575 µg/mL, 408 µg/mL and 250 µg/mL. Based on the results, the fractions derived from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. leaves exhibit antioxidant. The Fraction II from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. was the most cytotoxic on HeLa, T47D and MCF7 cell lines. It is potential to undergo further isolation of its cytotoxic compounds.
Keywords : antioxidant, cytotoxic, Plectranthus amboinicul (Lour.) Spreng., ethyl acetate fractions
Full Text:
PDFReferences
Anlar, H., Bacanli, M., Kutluk, B., Basaran, A.H. and Basaran, N., 2016, Cytotoxic Activity of Resveratrol in Different Cell Lines Evaluated by MTT and NRU Assays, Turk. J. Pharm. Sci., 13(1), 27-34. Link
Atolani, O. and Olantuji, G., 2016, Chemical Composition, Antioxidant and Cytotoxic Potential of Daniellia oliveri (Rolfe) Hutch. & Dalz, Turk. J. Pharm. Sci., 13(1), 41-46. Link
Asrin, H., Hasibuan, P.A.Z. and Marianne, 2017, Total Phenolic Content of Ethanol Extract of Artocarpus camansi Leave and Its Effect to Superoxide Dismutase (SOD) Level in Mice, Indones J. Cancer Chemoprevent., 8(3), 101- 109. CrossRef
Choirunnisa, A.R., Fidrianny, I. and Ruslan, K., 2016, Comparison of Five Antioxidant Assays for Estimating Antioxidant Capacity from Three Solanum Sp Extracts, Asian J. Pharm. Clin. Res., 9(Supl2), 123-128. CrossRef
Cicco, N., Lanorte, M.T., Paraggio, M., Viggianoa, M. and Lattanzio, V., 2009, A Reproducible, Rapid, and Inexpensive Folin-ciocalteu Micro-method in Determining Phenolies of Plant Methanol Extracts, Microchem. J., 91(1), 107-110. CrossRef
Dalimunthe, A., Hasibuan, P.A.Z., Silalahi, J., Sinaga, S.F. and Satria, D., 2018, Antioxidant Activity of Alkaloid Compounds from Litsea cubeba, Lour, Orient. J. Chem., 34(2), 1149-1152. CrossRef
Elgadir, M. A., Salama, M. and Adam, A.I.S.H.A.H., 2015, Anti-breast Cancer from Various Natural Sources Review, Int. J. Pharm. Pharm. Sci., 7(2), 44-47. Link
Geetha, S., Irulandi, K. and Mehalingam, P., 2017, Evaluation of Antioxidant and Free Radical Scavenging Activities of Different Solvent Exracts of Leaves of Piper umbellatum, Asian J. Pharm. Clin. Res., 10(2), 274-276. CrossRef
Gupta, M., Dahiya, J., Marhawa, R.M. and Dureja, H., 2015, Therapies in Cancer Treatment: an Overview, Int. J. Pharm. Pharm. Sci., 7(4), 1-9. Link
Illian, D. N., Basyuni, M., Wati, R. and Hasibuan, P.A.Z., 2018, Polyisoprenoids from Avicennia marina and Avicennia lanata inhibit WiDr cells proliferation, Pharmacogn. Mag., 14(58), 513. CrossRef
Hasibuan, P.A., Rosidah, Ilyas, S. and Nasution, M.P., 2013, Antioxidant and Cytotoxic Activities of Plectranthus amboinicus, (Lour.) Spreng. Extracts, Int. J. Pharm. Pharm. Res., 4(3), 755- 758. Link
Hasibuan, P.A.Z. and Rosidah, 2016, Combination Effect of Ethylacetate Extract of Plectranthus amboinicus, (Lour.) Spreng. With Doxorubicin Againts HeLa Cell Lines, Int. J. Pharm. Clin. Res., 8(5) Suppl, 357-360. Link
Hasibuan, PAZ, Chrestella, J. and Satria, D., 2015, Combination Effect of Ethylacetate Extract of Plectranthus amboinicus, (Lour.) Spreng. With Doxorubicin Againts T47D Breast Cancer Cells, Int. J. Pharm. Pharm. Sci., 7(10),156-159. Link
Hameed, H., Aydin, S., Başaran, A.A. and Başaran, N., 2016, Assessment of Cytotoxic Properties of Sinapic Acid in vitro, Turk. J. Pharm. Sci., 13(2), 225-232. Link
Kamuhabwa, A., Nshimo, C. and de Witte, P., 2000. Cytotoxic of Some Medicinal Plant Extracts Used in Tanzanian Traditional Medicine, J. Ethnopharmacol., 70(4), 143-149. Link
Mahadev, R., Ramakrisnaiah, H., Krishna, V., Deepalakhsmi, P. and Kumar, N.N., 2015, Cytotoxic Activity of Methanolic Extracts of Solanum erianthum, D. Don, Int. J. Pharm. Pharm. Sci., 7(2), 106-108. Link
Middleton, E., Kandaswami, C. and Theoharides T.C., 2000, The Effect of Plant Flavonoids on Mammalian Cells: Implication for Inflammation, Heart Disease and Cancer, Pharmacol. Rev., 52(4), 673-751. Link
Pokorny, J., Nedyalka, Y. and Michael, G., 2001, Antioxidants in Food, England: Woodhead Publishing Limited. Link
Rosidah and Hasibuan, P.A.Z, 2014, Cytotoxic Effect of n-Hexane, Ethylacetate, and Ethanol Extracts of Plectranthus amboinicus, (Lour.) Spreng. on HeLa and Vero Cells Lines, Int. J. Pharm. Tech. Res., 6(6), 1806-1809. Link
Rosidah, Yam, M.F., Sadikun, A. and Asmawi, M.Z., 2008, Antioxidant Potential of Gynura procumbens, Pharm. Biol., 46(9), 616-625. CrossRef
Satria, D., Silalahi, J., Haro,G.,Ilyas, S. and Hasibuan, P.AZ., 2017, Antioxidant and Antiproliferative Activity of Ethylacetate Fraction of Picria Felterrae, Lour. Herb, Asian Pac. J. Cancer Prev., 18(2), 399-403. CrossRef
Sitorus, P., Hasibuan, P.A.Z. and Satria, D., 2017, Total Phenolic and Flavonoid Contents and Antioxidant Acitivity of Ethanol Fraction of Picria fel-terrae, Lour. Herbs, Asian J. Pharm. Clin. Res., 10(7), 243-245. CrossRef
Sun, T. and Ho, C., 2005, Antioxidant Activities of Buckwheat Extracts, Food Chem., 90, 743-749. CrossRef
Thangavelu, K., Ravisankar, N., Siddiq, A. and Joseph, J, 2015, In Vitro Antioxidant and Anticancer Potential of Flowers of Toddalia Asiatica (Rutaceae), Int. J. Pharm. Pharm. Sci., 7(3), 95-99. Link
Wang, X., Zhang, F., Yang, L. and Mei, Y., 2011, Ursolic Acid Inhibits Proliferation and Induces Apoptosis of Cancer Cells In Vitro and In Vivo, J. Biomed. Biotechnol., 1, 1-8. CrossRef
DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev10iss1pp37-45
Copyright (c) 2019 poppy anjelisa zaitun hasibuan
Indexed by:
Indonesian Society for Cancer Chemoprevention