Banana Peels (Musa paradisiaca L.) Extract as Phytoestrogen on Ovariectomized Mice Mammary Gland Development by Inducing c-Myc Expression

Nanda Resa Pratama, Yurista Gilang, Rita Riata, Adam Hermawan, Muthi' Ikawati, Edy Meiyanto


Hormone Replacement Therapy (HRT) is therapy for estrogen deficiency and post menopausal syndromes, but high cost and unwell-secured therapy. One of alternative therapy is the usage of phytoestrogens. The banana peel contains flavones, flavonol, flavanone and polimethoxyflavone which are potential as phytoestrogen. The purpose of this study was to examine the estrogenic effect of banana peel extract (BPE) development of mammary gland of ovariectomized rats. Estrogenic effects was examined based on in vivo and in silico experiment. For in vivo experiment, female Sprague-dawley rats aged 50 days were ovariectomized. At 70 days of age, 12 rats were treated with BPE 500 mg/kgBB and 1000mg/kgBB, 5 rats were treated with estradiol 2μg/day while others served as control were treated with CMC-Na 0.5% and sacrificed 2 weeks later. The base line ovariectomized rats and base line non-ovariectomized rats were sacrificed at 70 days of age. The in silico experiment examined by molecular docking between myricetin and estrogen receptor alpha (ER-α). The result of in vivo experiment showed that 1000 mg/kgBW BPE induced c-Myc expression and enhance ovariectomized rat mammary gland development significantly. Meanwhile, molecular docking showed that there are hydrogen bond interaction between bioactive compound in BPE and Estrogen Receptor (ER)-α but less powerfull than estrogen and ER-α interaction. In summary, BPE can act as an estrogen agonist, resulting in the enhancement of c-Myc expression.

Keywords: banana peels extract (BPE), phytoestrogen, mammary gland, ovariectomized rats

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Achadiat, C.M., 2003, Fitoestrogen untuk Wanita Menopause,, accessed September 2010.

Chen X, Danes C, Lowe M, Thaddeus W, Herliezek, and Keyomarsi K., Activation of the Estrogen-Signaling Pathway by p21WAFI/CIPI in Estrogen Receptor-Negative Breast Cancer Cells. J. Natl. Cancer Inst. 2000; 92:17:1403-13.

Doisneau-Sixou, SF., Estrogen and Antiestrogen Regulation of Cell Cycle Progression in Breast Cancer Cells. Endrocrine-Related Cancer. 2003;10: 179-86.

Gardner L, Lee L, and Dang C. The c-Myc Oncogenic Transcription Factor. Encyclopedia of Cancer. 2002:2.

Jordan, C. V., Selective Estrogen Receptor Modulation : Concept and Consequences in Cancer. 2004; 5:207-13.

Maggiolini, M, Recchia, B., Catalano, V., Carpino, R.V, Rossi, R, and Ando S. The red wine phenolics piceatannol and myricetin act as agonists for estrogen receptor α in human breast cancer cells. Journal of Molecular Endocrinology. 2005;35:269-281.

Murkies AL, Gisela W, and Susan RD. Phytoestrogen. Journal of Endrocrinology and Metabolism. 1998; 83:2:297-303.

Sultana, B., Farooq, A., Muhammad, R.A., and Shahzad, ASC. Antioxidant potential of extracts from different agro wastes: Stabilization of corn oil. Grasas Y Aceites. 2008; 59:3:205-17.

Van der Woude H, Ter Veld MG, Jacobs N, Van der Saag PT, Murk AJ, and Rietjens IM. The Stimulation of Cell Proliferation by Quercetin is Mediated by The Estrogen Receptor. Mol. Nutr. Food Res. 2005; 49:8:763-71.


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