The Effect of Liberica Coffee on Liver Physiology and Histological Appearance of White Mice

Cindra Ayuning Kirana, Syamsurizal Syamsurizal, Diah Tri Utami

Abstract


Indonesia is one of the world's top coffee producing and exporting countries. Liberica coffee was one of the most widely cultivated types of coffee in Indonesia, especially in Tanjung Jabung Barat Jambi Province. Liberica coffee known as the coffee with highest levels of caffeine if compared with Arabica and Robusta coffee but the research about Liberica coffee was still very little if compared to the other two types of coffee. In the previous research, caffeine shown to be increasing alanine aminotransferase enzyme. Otherwise, coffee also contain chlorogenic acid which is a potent antioxidant and play a role in the protection of liver. The aim of this study are to investigate the effect of dark roasted Liberica coffee consumption on liver histological appearance, macroscopic anatomy and physiological function (serum glutamic-pyruvic transaminase (SGPT) level). This was an experimental study with posttest only control group design, using 45 male white mice and divided into 4 groups: control (with no coffee treatment), P1, P2 and P3 which were given coffee treatment consecutively 8000 ppm; 10.000 ppm and 12.000 ppm for 28 days. The result showed that liver histological damage level associated with the extent coffee intake. Although, the damage shown is still in a low level and has no impact in increasing SGPT level.

Keywords : Liberica coffee, Liver, Histological appearance, SGPT level


Full Text:

PDF

References


Aditya, I.W., Nocianitri, K.A. and Yusasrini, N.L.A., 2016, Kajian Kandungan Kafein Kopi Bubuk, Nilai pH dan Karakteristik Aroma dan Rasa Seduhan Kopi Jantan (Pea Berry Coffee) dan Betina (FlatBeans Coffee) Jenis Arabika dan Robusta, Jurnal Fakultas Teknologi Pangan, 5(1), 1-12. Link

Ananda, Sipahutar, H. and Nugrahalia, M., 2014, Daya Fertilitas Mencit (Mus musculus) Betina Pasca Pemberian Air Seduhan Kopi Peroral, Proceeding, Padang: Seminar Nasional Biodiversitas dan Ekologi Tropika Indonesia, pp.6-9. Link

Asikin, N., 2001, Antioksidan Endogen dan Penilaian Status Oksidan, Jakarta: Repository Fakultas Kedokteran Universitas Indonesia.

Bhara, M., 2004, Pengaruh Pemberian Kopi Dosis Bertingkat Per Oral 30 Hari Terhadap Gambaran Histologi Hepar Tikus Wistar, Thesis, Semarang: FK Universitas Diponegoro.

Boekschoten, M.V., Schouten, E.G., Katan, M.B, 2004, Coffee Bean Extract Rich and Poor in Kahweol Both Rise to Elevation of Liver Enzyms in Healthy Volunters, Nutr. J., 3, 7.CrossRef

Daswin, N. and Samosir, N.E., 2013, Pengaruh Kafein Terhadap Kualitas Tidur Mahasiswa Fakultas Kedokteran Universitas Sumatera Utara. E-Jurnal FK-USU, 1(1), 1341.

Dialab, 2006, Liquid Reagents of GOT (AST), Austria: DIALAB Production von chemish-technishen.

Fajariyah, S., Utami, E.T. and Arisandi, Y., 2010, Efek Pemberian Estrogen Sintetis (Diethylstillbestrol) Terhadap Struktur Hepar dan Kadar SGOT dan SGPT pada Mencit (Mus musculus) Betina Strain Bal/C, J. Ilmu Dasar, 11(1), 76-82.

Handajani, F., 2008, Pengaruh Pemberian Ekstrak Buah Merah (Pandanus conoideus L) pada Kadar SGOT dan SGPT Tikus Putih yang Diinduksi Parasetamol Dosis Tinggi Tunggal, Surabaya: Universitas Airlangga.

Hermanto S., 2007, Kafein Senyawa Bermanfaat atau Beracunkah, Jakarta: [no publisher].

Johnston K.L., Clifford M.N., Knight S. and Kuhnert N., 2003, Hierarchical Scheme for LC-MSn Identification of Chlorogenic Acids, J. Agric.Food Chem., 51(10), 2900-2911. CrossRef

Kee, J.L., 2007, Pedoman Pemeriksaan Laboratorium dan Diagnostik, 6th Ed, Jakarta: Penerbit Buku Kedokteran EGC.

Kreicbergs, V., Dimins, F., Mikelsone, V. and Cinkmanis, I., 2011, Biologically Active Compounds in Roasted Coffee, Proceeding, Latvia: Foodbalt 2011, pp.110-115.

Lee, K.A., Chae, J.I. and Shim, J.H., 2012, Natural Diterpenes from Coffee, Cafestol and Kahweol Induce Apoptosis through Regulation of Specificity Protein 1 Expression in Human Malignant Pleural Mesothelioma, J. Biomed.Sci., 19,, 60. CrossRef

Li, S.Y., Chang, C.Q., Ma, F.Y. and Yu, C.L., 2009, Modulating Effects of Chlorogenic Acid on Lipids and Glucose Metabolism and Expression of Hepatic Peroxisome Proliferator-Activated Receptor-Alpha in Golden Hamsters Fed on High Fat Diet, Biomed. Environ.Sci., 22(2),122-9. CrossRef

Maharani, P., 2007, Histopatologi Organ Hati dan Mata Pada Tikus Penderita Diabetes Mellitus Eksperimental, Essay, Bogor: Fakultas Kedokteran Hewan Institut Pertanian Bogor.

Muntiha, M., 2001, Teknik Pembuatan Prepatar Histopatologi dari Jaringan Hewan dengan Pewarnaan Haematoksilin dan Eosin (H&E), Proceeding, Bogor: Temu Teknis Fungsional Non

Peneliti, pp. 156-163.

Murase, T., Misawa, K., Minegishi, Y., Aoki, M., Ominami, H., Suzuki, Y., Shibuya, Y., et al., 2011, Coffee Polyphenols Suppress Diet-Induced Body Fat Accumulation by Downregulating

SREBP-1C and Related Molecules in C57BL/6J Mice, Am. J. Physiol. Endocrinol. Metab., 300(1), E122-E133. CrossRef

Nagao, T., Ochiai, R., Watanabe, T., Kataoka, K., Komikado, M., Tokimitsu, I., et al., 2009, Visceral Fat-reducing Effect of Continuous Coffe Beverage Consumption in Obese Subjects, Japan. Pharmacol.Ther., 37(3), 33-44.

Najiyati, S., and Danarti, 2009, Kopi: Budidaya dan Penanganan Lepas Panen, Jakarta: Penebar Swadaya.

Narita, Y. and Inouye, K.J., 2009, Kinetic Analysis and Mechanism on the Inhibition of Chlorogenic Acid and Its Components Against Porcine Pancreas Alpha-Amylase Isozymes I and II, J. Agric. Food Chem., 57(19), 18-25. CrossRef

Ong, K.W., Hsu, A. and Tan, B.K., 2012, Chlorogenic Acid Stimulates Glucose Transport in Skeletal Muscle Via APMK Activation: A Contributor to the Beneficial Effects of Coffee on Diabetes, PLOS One, 7(3), e32718. CrossRef

Ridwansyah, 2003, Pengolahan Kopi, Medan: Departemen Teknologi Pertanian, Fakultas Pertanian, Universitas Sumatera Utara.

Riswanto, A.T., 2009, Tinjauan Klinis Hasil Pemeriksaan Laboratorium, 11th Ed, Jakarta: Penerbit Buku Kedokteran EGC.

Robbins, S.L. and Kumar, V. 2001, Buku Ajar Patologi I (Basic Pathology Part II), 4th Ed, Jakarta: Penerbit Buku Kedokteran EGC.

Shimoda, H., Seki, E. and Aitani, M., 2006, Inhibitory Effect of Green Coffee Bean Extract on Fat Accumulation and Body Weight Gain in Mice, BMC Complement. Altern. Med., 6, 1-9. CrossRef

Siti, K., Laraswati, M. and Zatul, S., 2015, Analisis Konflik Laten Jepang-Cina. Peluang, Hambatan dan Kebijakan Ekspor Kopi Indonesia ke Pasar Amerika Serikat, Jurnal Ilmu Hubungan Internasional, 3(1), 758-760.

Sudiono, S., Hidayat. S., Rusmilah, S. and Soemartono, S., 2001, Deteksi Molekuler dan Uji Kisaran Inang Virus Gemini Asal Tanaman Tomat, Proceeding, Bogor: Konggres Nasional XVI, PFI, ISBN 979-95938-1-6, pp.210-216.

Wresdiyati, T., 2006, Profil Imunohistokimia Superoksida Dismutasi (SOD) Pada Jaringan Hati Tikus dengan Kondisi Hiperkolesterolemia, Hayati, 13(3), 85-89. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev9iss3pp%25p

Copyright (c) 2018 Cindra Ayuning Kirana

Indexed by:

               

                  

         

 

Indonesian Society for Cancer Chemoprevention