Mangifera indica L. Leaves Extract Induced Intrinsic Apoptotic Pathway in MCF-7 Cells by Decreasing Bcl-2 Expression and Inducing Bax Expression

Fitriasih Fitriasih, Siti Mega Komariyah, Ferry Sandra, Nenni Pratiwi, Devi Nisa Hidayati


Extract of Mangifera indica L. has been investigated to have cytotoxic activity against MCF-7 cells.  Current research was conducted to determine the cytotoxic activity of methanol extract of M. indica leaves (MEML) on MCF-7 breast cancer cells and to investigate on the MEML's effect on Bcl-2 and Bax expressions. M. indica leaves were extracted using soxhletation with methanol. The cytotoxic test was perfomed using MTT assay with various MEML concentrations: 15.625, 31.25, 62.5, 125, 250 and 1000 μg/mL. Meanwhile, Bax and Bcl-2 expressions of MCF-7 breast cancer cells were detected with immunocytochemical staining after treatment of MEML with concentrations of ¼ IC50, ½ IC50 and IC50. The results showed that IC50 of cytotoxic activity was 308.12 µg/mL. MEML could influence Bax and Bcl-2 protein expressions. After treatment of MEML, Bcl-2 expression was decreased while Bax expression was increased.

Keywords : Mangifera indica L., Cytotoxic, Bax, Bcl-2, MCF-7

Full Text:



Abdullah, A H., Mohammed, A S., Abdullah, Rasedee., Mirghani, Mohamed E S., and Al-Qubaisi, Mothanna., 2014, Cytotoxic effects of Mangifera indica L. Kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bioactive constituents in the crude extract, BMC Complement. Altern. Med., 14, 199. CrossRef

Adefolaju, G.A., Theron, K.E. and Hosie, M.J., 2015, BAX/BCL-2 mRNA and protein expression in human breast MCF-7 cells exposed to drug vehicles-methanol and dimethyl sulfoxide (DMSO) for 24 hrs, Niger. Med. J., 56(3), 169-174. CrossRef

Barreto, J.C., Trevisan, M.T., Hull, W.E., Erben, G., de Brito, E.S., Pfundstein, B., et al., 2008, Characterization and Quantitation of Polyphenolic Compounds in Bark, Kernel, Leaves, and Peel of Mango (Mangifera indica L.), J. Agric. Food Chem., 56(14), 5599-5610. CrossRef

International Agency for Research on Cancer, World Health Organization, Globocan, 2018, Website,, accessed on May 25, 2015. Link

Hendarmin, L., Kawano, S., Yoshiga, D., Sandra, F., Mitsuyasu, T., Nakao, Y., et al., 2008, An Anti-apoptotic Role of NF-κB in TNFα-induced Apoptosis in an Ameloblastoma Cell Line, Oral Sci. Int., 5(2), 96-103. CrossRef

Igney, F.H. and Krammer, P.H., 2002, Death and anti-death: tumor resistance to apoptosis, Nat. Rev. Cancer, 2(4), 277-288. Link

Núñez Selles, A.J., Daglia, M. and Rastrelli, L., 2016, The potential role of mangiferin in cancer treatment through its immunomodulatory, anti-angiogenic, apoptopic, and gene regulatory effects, Biofactors, 42(5), 475-491. CrossRef

Pan, L.L., Wang, A.Y., Huang, Y.Q., Luo, Y. and Ling, M., 2014, Mangiferin Induces Apoptosis by Regulating Bcl-2 and Bax Expression in the CNE2 Nasopharyngeal Carcinoma Cell Line, Asian Pac. J. Cancer Prev., 15(17), 7065-7068. CrossRef

Sandra, F., Nakamura, N., Mitsuyasu, T., Shiratsuchi, Y. and Ohishi, M., 2001, Two relatively distinct patterns of ameloblastoma: an anti-apoptotic proliferating site in the outer layer (periphery) and a pro-apoptotic differentiating site in the inner layer (centre), Histopathology, 39(1), 93-98. CrossRef

Sandra, F., Sudiono, J., Sidharta, E.A. Sunata, E.P., Sungkono, D.J., Dirgantara, Y., et al., 2014, Conditioned Media of Human Umbilical Cord Blood Mesenchymal Stem Cell-derived Secretome Induced Apoptosis and Inhibited Growth of HeLa Cells, Indones. Biomed. J., 6(1), 57-62. CrossRef

Sandra, F., 2018, Targeting Ameloblatoma into Apoptosis, Indones. Biomed. J., 10(1), 35-39. CrossRef

Sandra, F. and Sidharta, M.A., 2018, Caffeic Acid Induced Apoptosis in MG63 Osteosarcoma Cells Through Activation of Caspases, Mol. Cell. Biomed. Sci., 1(2): 1-5. CrossRef


Copyright (c) 2019

Indexed by:




Indonesian Society for Cancer Chemoprevention