Mangifera indica L. Leaves Extract Induced Intrinsic Apoptotic Pathway in MCF-7 Cells by Decreasing Bcl-2 Expression and Inducing Bax Expression

Fitriasih Fitriasih, Siti Mega Komariyah, Ferry Sandra, Nenni Pratiwi, Devi Nisa Hidayati

Abstract


Extract of Mangifera indica L. has been investigated to have cytotoxic activity against MCF-7 cells.  Current research was conducted to determine the cytotoxic activity of methanol extract of M. indica leaves (MEML) on MCF-7 breast cancer cells and to investigate on the MEML's effect on Bcl-2 and Bax expressions. M. indica leaves were extracted using soxhletation with methanol. The cytotoxic test was perfomed using MTT assay with various MEML concentrations: 15.625, 31.25, 62.5, 125, 250 and 1000 μg/mL. Meanwhile, Bax and Bcl-2 expressions of MCF-7 breast cancer cells were detected with immunocytochemical staining after treatment of MEML with concentrations of ¼ IC50, ½ IC50 and IC50. The results showed that IC50 of cytotoxic activity was 308.12 µg/mL. MEML could influence Bax and Bcl-2 protein expressions. After treatment of MEML, Bcl-2 expression was decreased while Bax expression was increased.

Keywords : Mangifera indica L., Cytotoxic, Bax, Bcl-2, MCF-7


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References


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DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev10iss1pp1-6

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