Antioxidant-Free Radical Scavenging of Some Euphorbiaceae Herbs

Titik Sunarni, Fransiska Leviana


In order to screen natural antioxidant, the research about antioxidant of some Euphobiaceae herbs, have been conducted. The air-dried herbs  of Euphorbia heterophylla L, Phyllanthus acidus (L.) Skeels, and Phyllanthus buxifolius Muell Arg were extracted with metanol. The obtained extract was concentrated and then suspended to produce n-hexane, ethyl acetat and aqueous fractions. Free radical scavenger activity against DPPH (1,1-diphenyl-2-pycrylhydrazyl) measured by spectrophotometric method  and the IC50 value was determined. The compounds of  active fraction had been identified by TLC method. All of the herbs showed activity as DPPH scavenger. Among these herbs, Euphorbia heterophylla L. and Phyllanthus buxifolius Muell, Arg. exhibited a strong free radical scavenging of ethyl acetat fraction with IC50 value 5,88 µg/ml and 4,64 µg/ml. The result of TLC by mobile phase n-buthanol-acetic acid-water (4:1:5) and acetic acid 15% showed flavonoid compound.

Keywords: Euphorbiaceae herbs, antioxidant, DPPH, flavonoid

Full Text:



Bartosikova, L., Necas, J., Suchy, V., Kubinova, R., Vesela, D., Benes, L., Illek, J., Salplachta, J., Florian, T., Frydrych, M., Klusakova, J., Bartosik,T., Frana, L., Frana, P., and Dzurova, J. 2003. Antioxidantive Effects of Morine in ischemia-Reperfusion of Kidneys in the Laboratory Rat. Acta Vet. BRNO, 72:87-94.

Bergman, M., Varshavsky, L., Gottlieb, H. E. and Grossman, S., 2001, The Antioxidant Activity of Aqueous Spinach Extract: Chemical Identification of Active Fractions, Phytochemistry, 58: 143-152.

Cos, P., Calomme, M., Sindambiwe, J.B., Bruyne, T.D., Cimanga, K., Pieters, L., Vlietinck, A.J., and Berghe, D.V., 2001, Cytotoxicity and Lipid Peroxidation-Inhibiting Activity of Flavonoids, Planta Med., 67 : 515-519.

Duh, P.D., Y.Y. Tu and G.C. Yen, 1999, Antioxidant activity of water extract of harng jyur (Chrysanthemum morifolium Ramat). Lebensm, -Wiss.u-Technol. 32:269-277.

Gulcin, I., Uguz, M.T., Oktay, M., Beydemir, S., and Kufrevioglu, O.I. 2004, Evaluation of the Antioxidant and Antimicrobial Activities of Clary Sage (Salvia sclarea L.). Turk I. Agric. For. 28: 25-33.

Kwon, Y.S. and Kim, C.M., 2003, Antioxidant Constituents from the Stem of Sorghum bicolor, Arch. Pharm. Res., 26 (7) : 535 – 539.

Oke, J.M. and Hamburger, M.O., 2002, Screening of Some Nigerian Medicinal Plants For Antioxidant Activity Using 2,2, Diphenyl-Picryl-Hydrazyl Radical, AJBR, 5 : 77-79.

Pokorny, J., Yanishlieva, N. and Gordon, M., 2001, Antioxidants in Food, Practical Applications, 1-123, Wood Publishing Limited, Cambridge, England.

Yagi, A., Kabash, A., Okamura, N., Haraguchi, H., Moustafa., S.M., and Khalifa., T.I., 2002, Antioxidant, Free Radical Scavenging and Anti-inflammatory Effect of Aloesin Derivatives in Aloe vera, Planta Med., 68 : 957-960.


Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:





Indonesian Society for Cancer Chemoprevention