Estrogenic Activity of Ethanolic Extract of Papaya Peels (Carica Papaya L.) on Uterine Weight and Mammae Gland Proliferation on Ovariectomy Rats

Dhania Novitasari, Devyanto Hadi Triutomo, Fitriana Hayyu Arifah, Anselma Ivanawati, Zahrotul Ulum, Retno Murwanti

Abstract


Papaya bark is one of Indonesia's natural wealth that contains flavonoid compounds such as myricetin and kaempferol that included in the phytoestrogen compounds. The aim of this study is to examine the estrogenic effects of ethanolic extract of papaya peels (EEPP) on the development of mammae gland and the increasing of uterine weight. The in vivo test was performed in ovariectomized Sprague Dawley female rats. After 30 days of treatment, animals were sacrificed to take the uterus and mammae glands. Measurement of uterine weight and mammae gland was observed by hematoxylin-eosin staining method to know the lobulus development and AgNOR staining to determine the proliferation level of mammae gland epithelial cells. The results showed that EEPP at the concentrations of 500 and 1000 mg/kgBW were able to increase uterine weight and proliferation of mammae gland. In conclusion, papaya bark has the potential as phytoestrogen compound to maintain female reproductive health and woman beauty.

Keywords : Ethanolic extract of papaya peels (EEPP), phytoestrogen, ovariectomized rats, uterine weight, mammae proliferation


Full Text:

PDF

References


Chlebowski, R.T., Lewis, H.K., Ross, L.P., Marcia, L.S., JoAnn, E.M., Margery, G., et al., 2009. Breast Cancer after Use of Estrogen plus Progestin in Postmenopausal Women, N. Engl. J. Med., 360(6), 573-587, CrossRef.

Frasor, J., Barnett, D.H., Danes, J.M., Hess, R., Parlow, A.F. and Katzenellenbogen, B.S., 2003, Response-Specific and Ligand Dose Dependent Modulation of Estrogen Reseptor (ER) Alpha Activity by ER Beta in the Uterus, Endocrinology, 144(7), 3159-3166, CrossRef.

Jordan, V., 2004, Selective Estrogen Receptor Modulation: Concept and Consequences in Cancer, Cancer Cel., 5(3), 207-213, CrossRef.

Maggiolini, M.A., Recchia, G., Bonofiglio, D., Catalano, S., Vivacqua, A., Carpino, A., et al., 2005, The Red Wine Phenolics Piceatannol and Myricetin Act as Agonists for Estrogen Receptor in Human Breast Cancer Cells, J. Clin. Mol. Endocrinol., 35, 268-281, CrossRef.

Mendelsohn, M.E. and Karas, R.H., 2010, Rapid Progress for Non-nuclear Estrogen Receptor Signaling, J. Clin. Invest., 120(7), 2277-2279, CrossRef.

Oh, S.M., Kim, Y.P. and Chung, K.H., 2006, Biphasic Effects of Kaempferol on the Estrogenicity in Human Breast Cancer Cells, Arch. Pharm. Res., 29(5), 354-362, CrossRef.

Pratama, N.R., Gilang, Y., Riata, R., Hermawan, A., Ikawati, M. and Meiyanto, E., 2011, Banana Peels (Musa paradisiaca L.) Extract as Phytoestrogen on Ovariectomized Mice Mammary Gland Development by Inducing c-Myc Expression, Indones. J. Cancer Chemoprevent., 2(1), 151-159, CrossRef.

Rivera-Pastrana, D.M., Yahia, E.M. and González-Aguilar, G.A., 2010, Phenolic and Carotenoid Profiles of Papaya Fruit (Carica papaya L.) and their Contents Under Low Temperature Storage, J. Sci. Food Agric., 14(90), 2358-2365, CrossRef.

Trivedi, R., Kumar, S., Kumar, A., Siddiqui, J.A., Swarnkar, G., Gupta, V., et al., 2008, Kaempferol has Osteogenic Effect in Ovariectomized Adult Sprague-Dawley Rats, Mol. Cell. Endrocinol., 289(1), 85-93, CrossRef.

Turner, C.D. and Bagnara, J.T., 1988, Endokrinologi Umum, Surabaya: Airlangga University Press.

Yildiz, F., 2005, Phytoestrogens in Functional Foods, Boca Raton: Taylor and Francis Ltd, Link.




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev9iss2pp86-91

Copyright (c) 2018 Dhania Novitasari, Devyanto Hadi Triutomo, Fitriana Hayyu Arifah, Anselma Ivanawati, Zahrotul Ulum, Retno Murwanti

Indexed by:

                 

                  

   

 

Indonesian Society for Cancer Chemoprevention