Ethanolic Extract of Hedyotis corymbosa L. Inhibits Migration and MMP-9 Activity on Metastatic Breast Cancer Cells

Dhania Novitasari, Sri Handayani, Riris Istighfari Jenie


Many natural products have been widely explored for their pharmacological activities, including anticancer activity. Hedyotis corymbosa L. is known for its anticancer properties toward several cancer cell lines. The study aims to investigate whether ethanolic extract of Hedyotis corymbosa L. (EEH) performs anticancer properties by inhibiting migration and metastasis on breast cancer cells. Cytotoxic evaluation by using MTT assay was carried out to determine EEH effect on 4T1 breast cancer cells, meanwhile to investigate the treatment of EEH in migration and metastasis inhibitory effect, scratch wound healing assay and gelatin zymography were conducted in this study. The data showed that EEH possessed cytotoxic activity with IC50 value of 400 μg/mL.  Interestingly, migration inhibitory effect was shown up to 42 hours and the activity of MMP-9 was also decreased after the treatment with EEH. According to these findings, we suggest that Hedyotis corymbosa L. promotes another anticancer properties by inhibiting migration and metastasis towards breast cancer cells.

Keywords : Hedyotis corymbosa L., cytotoxicity, migration, metastatic, 4T1 breast cancer cells

Full Text:



Andriyani, R., Risdian, C. and Udin, Z., 2011, Cytotoxicity Assay From Fractions of Hedyotis corymbosa Extract Against Breast Cancer Cell Line T47D, Indones. J. Cancer Chemoprevent., 2(1), 182-186. CrossRef

Elfahmi, Woerdenbag, H.J. and Kayser, O., 2014, Jamu: Indonesian Traditional Herbal Medicine towards Rational Phytopharmacological Use, J. Herbal Med., 30(2), 51-73. CrossRef

Harmand, P.O., Duval, R., Delage, C. and Simon, A., 2005, Ursolic Acid Induces Apoptosis through Mitochondrial Intrinsic Pathway and Caspase-3 Activation in M4Beu Melanoma Cells, Int. J. Cancer, 114(1), 1-11. CrossRef

Haryanti, S., Junedi, S. and Meiyanto, E., 2009, Ethanolic Extract of Hedyotis corymbosa L. Increases Cytotoxic Activity of Doxorubicin on MCF-7 Breast Cancer Cell, Indones. J. Biotechnol., 14(1), 1146-1154. CrossRef

Hsieh, C.Y., Tsai, P.C., Chu, C.L., Chang, F.R., Chang, L.S., Wu, Y.C., et al., 2013, Brazilein Suppresses Migration and Invasion of MDA-MB-231 Breast Cancer Cells, Chem. Biol. Interact., 204(2), 105-115. CrossRef

Hudis, C.A. and Gianni, L., 2011, Triple-negative Breast Cancer: An Unmet Medical Need, Oncologist, 16(Suppl 1), 1-11. CrossRef

Lee, H.Z., Bau, D.T., Kuo, C.L., Tsai, R.Y., Chen, Y.C. and Chang, Y.H., 2011, Clarification of the Phenotypic Characteristics and Anti-tumor Activity of Hedyotis Diffusa, Am. J. Chin. Med., 39(1), 201-213. CrossRef

Li, Y., Xing, D., Chen, Q. and Chen, W.R., 2010, Enhancement of Chemotherapeutic Agent Induced Apoptosis by Inhibition of NF-κB Using Ursolic Acid, Int. J. Cancer, 127(2), 462-473. CrossRef

Meiftasari, A., Pandega, J.C.W., Novarina, A., Wijayanti, J.Y. and Jenie, R.I., 2016, Ethanolic Extract of Hedyotis corymbosa and Its Combination with 5-FU Inhibit Cyclin D Expression on WiDr Colorectal Cancer Cell, Indones. J. Cancer Chemoprevent., 7(1), 25-30. CrossRef

Mosmann, T., 1983, Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxic Assays, J. Immunol. Methods, 65(1-2), 55-63. CrossRef

Noiarsa, P., Ruchiwarat, S., Otsuka, H. and Kanchanapoom, T., 2008, Chemical constituents from Oldenlandia corymbosa L. of Thai origin, J. Nat. Med., 62(2), 249-250. CrossRef

Nurcahyanti, A.D.R., 2016, Cervical Cancer: The Case in Indonesia and Natural Product-Based Therapy, J. Cancer Biol. Res., 4(1), 1078. Link

Pandey, K., Sharma, P.K. and Dudhe, R., 2012, Anticancer Activity of Parthenium hysterophorus Linn and oldenlandia corymbosa Lam by SRB Method, Open Access Sci. Rep., 1(6), 1-3. Link

Prasad, S., Yadav, V.R., Sung, B., Reuter, S., Kannappan, R., Deorukhkar, A., et al., 2012, Ursolic Acid Inhibits Growth and Metastasis of Human Colorectal Cancer in An Orthotopic Nude Mouse Model by Targeting Multiple Cell Signaling Pathways: Chemosensitization with Capecitabine, Clin. Cancer Res., 18(18), 4942-4953. CrossRef

Prasad, S., Yadav, V.R., Sung, B., Gupta, S.C., Tyagi, A.K. and Aggarwal, B.B., 2016, Ursolic Acid Inhibits the Growth of Human Pancreatic Cancer and Enhances the Antitumor Potential of Gemcitabine in an Orthotopic Mouse Model through Suppression of the Inflammatory Microenvironment, Oncotarget, 7(11), 13182-13196. CrossRef

Reis-Filho, J. S. and Pusztai, L., 2011, Gene Expression Profiling in Breast Cancer: Classification, Prognostication, and Prediction, Lancet, 378(9805), 1812-1823. CrossRef

Sivapraksam, S.S.K., Karunakaran, K., Subburaya, U. and Kuppusamy, S., 2014, A Review on Phytochemical and Pharmacological Profile of Hedyotis corymbosa Linn., Int. J. Pharm. Sci. Rev. Res., 26(1), 320-324. Link

Torre, L.A., Bray, F., Siegel, R.L., Ferlay, J., Lortet-Tieulent, J. and Jemal, A., 2015, Global Cancer Statistics, 2012, C.A. Cancer J. Clin., 65(2), 87-108. CrossRef

Wang, W., Zhao, C., Jou, D., Lü, J., Zhang, C., Lin, L. and Lin, J., 2013, Ursolic Acid Inhibits the Growth of Colon Cancer-initiating Cells by Targeting STAT3, Anticancer Res., 33(10), 4279-4284. Link

Xavier, C.P., Lima, C.F., Pedro, D.F., Wilson, J.M., Kristiansen, K. and Pereira-Wilson, C., 2013, Ursolic Acid Induces Cell Death and Modulates Autophagy through JNK Pathway in Apoptosis-resistant Colorectal Cancer Cells, J. Nutr. Biochem., 24(4), 706-712. CrossRef


Copyright (c) 2018 Dhania Novitasari, Sri Handayani, Riris Istighfari Jenie

Indexed by:




Indonesian Society for Cancer Chemoprevention