Total Phenolic Content of Ethanol Extract of Artrocarpus camansi Leave and its Effect to SOD (Superoxide Dismutase) Level in Mice
Abstract
A free radical is one of the triggers of degenerative diseases that become the biggest cause of death. Excessive production of free radicals can be neutralized by antioxidant. Antioxidants can be generated from within the body (intracellular). One of them by the enzyme SOD (superoxide dismutase). However, when the production of free radicals exceeds the ability of intracellular antioxidants to neutralize it, antioxidants from outside (extracellular) is necessary. The ethanol extract of Artocarpus camansi leaves (EEACL) contains phenolic compounds which has very strong antioxidant activity based on in vitro study using the DPPH method, but the in vivo study about the total phenolic content effect of its leaves toward antioxidant activity has not been done. 25 mice were divided into 5 groups consisting of control group, a group was induced by stress and three groups were induced by stress, but given EEACL with each dose of 50 mg/kg bw, 100 mg/kg bw and 150 mg/kg bw. Inducing stress in the form of psychological stress was carried out for 7 days and continued with the EEACL administration for 7 days. The mice were dissected and the livers were isolated, then the liver morphological was examined using Hematoxyllin Eosin (HE) staining method and SOD level was examined with immunohistochemical staining method. The data were analyzed with One Way ANOVA and Duncan test using SPSS program version 19.0. Total phenolic content of EEACL is 235.03 ± 4.306 mg GAE/ g of sample. The average SOD levels in the control group is 94.05 %, stressed group is 55.94 %, stress with EEACL dose 50 mg/kg bw group is 58.40 %, stress with EEACL dose 100 mg/kg bw group is 79.68 %, stress with EEACL dose 150 mg/kg bw group is 80.90 %. Based on statistical result, SOD level increased along with the increase of EEACL dose, but not significantly (p < 0,05). Total phenolic content of EEACL has an influence to SOD levels. SOD level increased along with the increase of EEACL administration dose. The higher dose of EEACL leading to higher levels of SOD in the mouse liver.
Keywords : total phenolic content, superoxide dismutase, antioxidant, Artocarpus camansi
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Anisman, H. and Merali, Z., 1999., Understanding Stress: Characteristic and Caveates, Alc. Res. Health., 23(4),241-248.
Borras, C., Gambini, J., Cabrera, M.C.G., Sastre, J., Pallardo, F.V, Mann, G.E., et al, 2006, Genistein, A Soy Isoflavone, Up Regulates Expression of Antioxidant Genes: Involvement of Estrogen Receptor, ERK1/2 and NFkB, FASEB Journal, 20(12), 1476-2138. CrossRef
Chida, Y., Sudo, N. and Kubo, C., 2006, Does Stress Excerbate Liver Diseases?, J. Gastroenterol. Hepatol., 21(1), 202-208. CrossRef
Gulcin, L., Uguz, M.T., Oktay, M., Beydemir, S. and Kufrevioglu, O.I., 2004, Evaluation of the Antioxidant and Antimicrobial Activities of Clary Sage (Savia sclarea L.), Turk. I. Agric. For., 28, 25-33.
Halliwell, B., 2006, Reactive Species and Antioxidants: Redox Biology is a Fundamental Theme of Aerobic Life, Plant Physiol., 141(2), 312-322. CrossRef
Halliwel, B., 2001, Free Radicals and Other Reactive Species Disease, In: Encyclopedia of Life Science, Chichester: John Wiley and Sons Ltd, pp. 1- 7. Link
Hilakivi-Clarke, L. and Dickson, R.B., 1995. Stress Influence on Development of Hepatocellular Tumors in Transgenic Mice Overexpressing TGF-α, Acta Oncol., 34(7), 907–912. CrossRef
Junqueira, L.C. and Carneiro, J., 2003, Basic Histology: Text & Atlas, Tenth Ed., In: Tambayong, J., translator, 2003 Histologi Dasar: Teks & Atlas, 10th edition, Jakarta: EGC Publisher.
Kregel, K.C. and Zhang, H.J., 2007, An Integrated View of Oxidative Stress in Aging: Basic Mechanisms, Functional Effects, and Pathological Considerations, Am. J. Physiol. Regul. Integr. Comp. Physiol., 292(1), R18-R36. CrossRef
Marianne, Yuandani, and Rosnani, 2011, Antidiabetic Activity From Ethanol Extract of Kluwih’s Leaf (Artocarpus camansi), Jurnal Natural, 11(2), 64-68.
Moein, S. and Mahmood, R.M., 2010, Relationship between Antioxidant Properties and Phenolics in Zhumeria majdae, J Med. Plants Res., 4(7), 517-521.
Nishio,Y., Nakano,Y., Deguchi, Y., Terato,H,. Ide,H., Ito,C., et al, 2007, Social Stress lnduces Oxidative DNA Damage in Mouse Peripheral Blood Cells, Genes Environt., 29(1), 17-22. CrossRef
Pietta, P.G, 2000. Flavonoids as Antioxidants, J. Nat. Prod., 63(7), 1043-1046. CrossRef
Rosidah, Yam, M.F. and Asmawi, M.Z., 2008, Antioxidant Potential of Gynura procumbens, Pharm. Biol., 46(9), 616-625.
Wiryanthini, D.I.A, Aman I.G.M, and Bagiada N.A, 2012, Pemberian Ekstrak Biji Kakao (Theobroma cacao l.) Menurunkan Kadar Malondialdehide dan Meningkatkan Kadar NOx Darah Tikus Putih (Rattus norvegicus) yang Diinduksi Stres Psikososial, Jurnal Ilmiah Kedokteran Medicina 43, 47.
Wresdiyati, T., Astawan,M., Fithriani, D., Adnyane,I.K.M, Novelia,S. and Aryani, S., 2009., Pengaruh α-Tokoferol terhadap profil Superoksida Dismutase dan Malondialdehida pada jaringan Hati Tikus di Bawah Kondisi Stress, Jurnal Veteriner, 10(2), 202-209.
DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev8iss3pp101-109
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