Combination of Curcuma (Curcuma xanthorriza Roxb) Rhizome Ethanolic Extract and Awar-Awar (Ficus septica Burm.F) Leaves Ethanolic Extract Increases Cisplatin Cytotoxicity on T47D Breast Cancer Cells through Cell Cycle Modulation

Devi Nisa Hidayati, Riris Istighfari Jenie, Edy Meiyanto

Abstract


Curcuma (Curcuma xanthorriza Roxb.) and Awar-awar ((Ficus septica Burm.f.) are well known to have anticancer potential especially for breast cancer with low toxicity. This study aims to examine the potential effect of the combination of Curcuma ethanolic extracts (CEE) and awar-awar leaves ethanolic extract (AEE) to increase the efficacy of cisplatin toward T47D breast cancer cells. The combination activity was done using 3 series of concentration, 1/3; 1/6 and 1/12 of IC50, to determine the combination index (CI) of cisplatin, CEE and AEE under MTT assay. The result showed that the combination of 2.5 µM, 5 µg/ml, 1 µg/ml concentrations of  cisplatin, CEE and AEE respectively result in synergistic effect with CI values less than 1. The treatment exhibited the cell accumulation in S phase (27.7%) against T47D breast cancer cells confirmed through cell cycle examination by flow cytometry. These results provided the evidence that the CEE and the AEE can be developed as co-chemotherapeutic agents combined with cisplatin to improve the effectiveness of breast cancer treatment.

Keywords : Curcuma xanthorriza Roxb., Ficus septica Burm.f., cisplatin, cell cycle


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References


Cruet-Hennequart, S., Villalan, S., Kaczmarczyk, A., O’Meara, E., Sokol, A.M., dan Carty, M.P., 2009. Characterization of The Effect of Cisplatin and Carboplatin on Cell Cycle Progression and DNA Damage Response Activation in DNA Polymerase Eta-deficient Human Cells. Cell cycle (Georgetown, Tex.), 8(18): 3039–3050. CrossRef

Dalimartha, S., 2008. Kandungan Nutrisi & Bioaktif Tahitain Noni Juice Membantu Mengatasi Kanker Payudara. Women Health Cancer, Jakarta.

Damu, A.G., Kuo, P.C., Shi, L.S., Li, C.Y., Su, C.R., dan Wu, T.S., 2009. Cytotoxic Phenanthroindolizine Alkaloids from the Roots of Ficus septica. Planta Med, 75(10): 1152–1156. CrossRef

Dhar, S.N., Kolishetti, S.., Lippard, dan Farokhzad, 2011. Re: Targeted Delivery of a Cisplatin Prodrug for Safer and More Effective Prostate Cancer Therapy In Vivo. Proceedings of the National Academy of Sciences, 108(5) 1850–1855. CrossRef

Florea, M.A. dan Busselberg, D., 2011. Cisplatin as an Anti-Tumor Drug : Celullar Mechanism of Activity, Drug Resistance and Induced Side Effects. Cancer, 3(4): 1351–1371. CrossRef

Foo, S.Y. dan Nolan, G.P., 1999. NF-kappaB to the Rescue: RELs, Apoptosis and Cellular Transformation. Trends Genet, 15(6): 229–235. CrossRef

IARC, 2013. Latest World Cancer Statistics Global Cancer Burden Rises To 14.1 Million New Cases In 2012: Marked Increase In Breast Cancers Must Be Addressed. World Health Organization.

Jemal, A., Bray, F., Mellisa, M.C., Ferlay, J., dan Ward, E., 2011. Global Cancer Statistic. CA Cancer J. Clin, 61: 43–66.

Kim, K.H., Park, H.Y., Nam, J.H., Park, J.E., Kim, J.Y., Park, M.I., dkk., 2005. The Inhibitory Effect of Curcumin on the Growth of Human Colon Cancer Cells (HT-29, WiDr) in Vitro. Korea J Gastroenterol, 4: 277–84.

Milosavlievic, N., Duranton, C., Djerbi, N., Puech, P., Gounon, P., LagadicGossmann, D., 2010. Nongenomic Effects Of Cisplatin: Acute Inhibition Of Mechanosensitive Transporters and Channels Without Actin Remodeling. Molecular and Cellular Pathobiology, 70: 7514–7522. CrossRef

Reynold, C.P. dan Maurer, B.J., 2005. Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models. Methods Mol, 110: 173–183. CrossRef

Sekti, D.A., Mubarok, M.F., Armandani, I., Junedy, S., dan Meiyanto, E., 2010. Ekstrak Etanolik Daun Awar–awar (Ficus septica Burm. f.) Memacu Apoptosis Sel Kanker Payudara MCF-7 Melalui Penekanan Ekspresi Bcl-2. Majalah Obat Tradisional, 15: 100–104.

Siegel, R., Ma, J., Zou, Z., dan Jemal, A., 2014. Cancer Statistics 2014. Cancer Journal for Clinicians, 64(1): 9–29. CrossRef

Ueda, J.Y., Tezuka, Y., Banskota, A.H., Tran, Q.L., Hariyana, Y., Saiki, I., dkk., 2002. Antiproliferative Activity of Vietnamase Medical Plants. Biol.Pharm, Bull, 25(6): 753–760. CrossRef

Zhao, L., Wientjes, M.G., dan Au, J.L., 2004. Evaluation of Combination Chemotherapy: Integration of Nonlinear Regression, Curve Shift, isobologram, and Combination Index Analyses. Clinical Cancer Research, 10(23): 7994-8004. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev8iss3pp120-125

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