A Comparison of Antimetastatic Activity between Nerium indicum and Cinnamomum burmannii on 4T1 Cells

Beni Lestari, Laeli Muntafiah, Ziana Walidah, Riris Istighfari Jenie

Abstract


Metastatic process becomes a major problem in advanced cancer cases. Natural compounds found in several plants in Indonesia have a potency to be developed as chemoterapeutic agent which are targeted to metastatic process. Jure leaves (Nerium indicum) which contain oleandrin and cinnamaldehyde in cinnamon bark (Cinnamomum burmannii) reported to have cytotoxic activity on several cancer cells, but their activities on metastasic process have never been explored. This research aims to reveal and to compare their anti-metastatic effect toward 4T1 breast cancer cells. The cytotoxicity of jure leaves extract (JLE) and cinnamon essential oil (CEO) was obtained by MTT assay. Metastatic process mainly on cell migration was examined by scratch wound healing assay while MMP-9 expression that described the invassion process was observed by gelatin zymography assay. Molecular interaction between their active compounds and MMP-9 receptor was predicted by molecular docking. The result showed that treatment with JLE and CEO inhibited the growth of 4T1 cells with IC50 value of 125 µg/mL and 2.5 µg/mL, respectively. In addition, JLE performed inhibitory effect of cell migration better than CEO. Meanwhile, both JLE and CEO decreased MMP-9 protein expression. Thus, JLE and CEO have potentials to be developed as an anti-metastatic agent and JLE could be more effective.

Key words: Nerium indicum, Cinnamomum burmannii, anti-metastasis, scratch assay, gelatin zimography


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References


Duke, J.A. and Boca Raton, 1985, Handbook of Medicinal Herbs, FL: CRC Press.

Hartwel, J.L., 1982, Plants Used Against Cancer, 407, Quarterman Publication, Inc., Lawrence, massachusetts.

Hembing, W.H.M., 1993, Tanaman Berkhasiat Obat di Indonesia, 2nd Edition, 74-75, Jakarta : Pustaka Kartini.

Heppner, G.H., Miller, F.R. and Shekhar, P.M., 2000, Nontransgenic Models of Breast Cancer, Breast Cancer Res., 2(5), 331–334. CrossRef

Hsieh, Y.S., Chu, S.C., Yang, S.F., Chen, P.N., Liu, Y.C. and Lu, K.H., 2007, Silibinin Suppresses Human Osteosarcoma MG-63 Cell Invasion by Inhibiting the ERK-dependentc- Jun/AP-1 Induction of MMP-2, Carcinogenesis, 28, 977–298. CrossRef

Hu, K., Liu, Q., Wang, S. and Ding, K., 2009. New Oligosaccharides Prepared by Acid Hydrolysis of the Polysaccharides from Nerium indicum Mill and Their Anti-angiogenesis Activities, Carbohydr. Res., 344(2), 198–203. CrossRef

Calderón-Montaño, J.M., Burgos-Morón, E., Orta, M.L., Mateos, S. and López-Lázaro, M., 2013, A Hydroalcoholic Extract from the Leaves of Nerium oleander Inhibits Glycolysis and Induces Selective Killing of Lung Cancer Cells, Planta Med., 79(12), 1017–1023. CrossRef

Koppikar, S.J., Choudhari, A.S., Suryavanshi, S.A., Kumari, S., Chattopadhyay, S. and Kaul-Ghanekar, R., 2010, Aqueous Cinnamon Extract (ACE-c) from the Bark of Cinnamomum Cassia Causes Apoptosis in Human Cervical Cancer Cell Line (SiHa) Through Loss of Mitochondrial Membrane Potential, BMC Cancer, 10, 210. CrossRef

Larasati, Y. A., Putri, D. D. P., Utomo, R. Y., Hermawan, A., and Meiyanto, E., 2014, Combination of Cisplatin and Cinnamon Essential Oil Inhibits HeLa Cells Proliferation through Cell Cycle Arrest, J. App. Pharm. Sci., 4(12), 014-019. CrossRef

Liao, L.B., Zhou, H.Y. and Xiao, X.M., 2005, Spectroscopic and Viscosity Study of Doxorubicin Interaction with DNA, J. Mol. Struct., 749(1-3), 108-113. CrossRef

Lu, J., Zhang, K., Nam, S., Anderson, R., Jove, R. and Wen, W., 2010. Novel Angiogenesis Inhibitory Activity in Cinnamon Extract Blocks VEGFR2 Kinase and Downstream Signaling, Carcinogenesis, 31(3), 481-488. CrossRef

McConkey, D.J., Lin, Y., Nutt, L.K., Ozel, H.Z. and Newman, R.A., 2000, Cardiac Glycosides Stimulate Ca2+Increases and Apoptosis in Androgen-independent, Metastat ic Human Prostate Adenocarcinoma Cells, Cancer Res., 60(14), 3807-3812. Link

Manna, S.K., Sah, N.K., Newman, R.A., Cisneros, A. and Aggarwal, B.B., 2000, Oleandrin Suppresses Activation of Nuclear Transcription Factor-κB, Activator Protein-1, and c-Jun NH2-Terminal Kinase, Cancer Res., 60(14), 3838 –3847. Link

Prayong, P., Barusrux, S. and Weerapreeyakul, N., 2008, Cytotoxic Activity Screening of Some Indigenous Thai Plants, Fitoterapia, 79(7-8), 598-601. CrossRef

Scoene, N. W., Kelly, M. A., Polansky, M. M. and Anderson, R. A., 2008, A Polyphenol Mixture from Cinnamon Targets p38 MAP Kinase-Regulated Signaling Pathways to Produce G2/M Arrest, J. Nutr. Biochem., 20(8), 614-620. CrossRef

Siddiqui, S., Hafeez, F., Begum, S. and Siddiqui, B.S., 1987, Isolation and Structure of Twocardiac Glycosides from the Leaves of Nerium oleander, Phytochemistry, 26(1), 237-241. CrossRef

Wahyuningsih, M. S. H., Wahyuono, S. and Artama, W. T., 2000, Efek Sitotoksik Oleandrin, Senyawa Bioaktif Hasil Isolasi dari daun Nerium indicum Mill. Terhadap Sel Mieloma, Berkala Ilmu Kedokteran, 32(4), 235-241. Link

Wahyuningsih, M.S.H., 2006, Selektivitas dan Mekanisme Antikanker 5a-oleandrin dan dehidrodeasetil-5a-oleandrin Hasil Isolasi daun jure (Nerium indicum Mill.), Disertation, Yogyakarta: Universitas Gadjah Mada. Link

Wang, R., Wang, R. and Yang, B., 2009, Extraction of Essential Oils from Five Cinnamon Leaves and Identification of Their Volatile Compound Compositions, Innov. Food Sci. Emerg. Techno., 10(2), 289-292. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev8iss2pp85-93

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