Acetoxy Chavicol Acetate (ACA) Concentration and Cytotoxic Activity of Alpinia galanga Extract on HeLa, MCF7 and T47D Cancer Cell Lines

Andi Suhendi, Erindyah Retno Wikantyasning, Gunawan Setyadi, Arifah Sri Wahyuni, Muhammad Da'i


Due to severe side effect and non-specific chemotherapeutic agent, screening and discovery for cancer therapy are still working, especially from natural resources. Traditionally, people used herbal medicine either to prevent or cure diseases. One of herbal that commonly used in Indonesia is Alpinia galangal. Previous study stated that active compound is acetoxy chavicol acetate (ACA) and active as anticancer. This research aimed to determine ACA concentration and cytotoxic activity of Alpinia galanga extract (AGE) from three local markets on HeLa, MCF7 and T47D cell lines. The galangal used from three local markets namely Pasar Legi Surakarta, Beringharjo Yogyakarta, and Wonogiri. The extraction was performed by maceration using 96% ethanol as solvent. ACA quantitation using UV spectrophotometer at λ = 208.5 nm. Samples were prepared by liquid-liquid extraction using an ethyl acetate. Cytotoxic activities were performed by MTT assay. The result showed that the concentration of ACA of AGE from the three local markets were 3.798; 0.035; and 0.009 % w/w, respectively. Cytotoxic activity, describes as IC50 value, on HeLa cell line of AGE from three local markets, in order were 13.26; 36.32 and > 100 µg/ mL. Meanwhile, AGE from Pasar Legi on MCF7 and T47D cell lines have IC50 value of 15.80; 12.50 µg/ mL, respectively. In contrast, two other samples have IC50 values of greater than 100 µg/ mL. The highest activity was from the highest concentration of ACA on the samples.

Key words: Alpinia galanga, HeLa, T47D, MCF7 and Acetoxy chavicol acetate

Full Text:



Asri, A. and Winarko, S., 2016, Antiproliferative Activity by Ethanolic Extract of Red Alpinia galanga (L) Willd in Inoculated Breast Carcinoma Cells of C3H Mice, J. Advances in Med. Pharm. Sci., 5(4), 1–9. CrossRef

Bermawie, N., Purwiyanti, S., Melati, M. and Meilawati N.W., 2012, Karakter Morfologi, Hasil, dan Mutu Enam Genotip Lengkuas pada Tiga Argoekologi, Bul. Littro, 23(2), 125–135. CrossRef

Chauhan Vimal Singh, Swapna M, and A.S., 2014. Phytochemical Investigation and Cytotoxic Activity Of Methanolic Extract of Alpinia galanga, Department of Pharmacology, Vidhya Bharathi College of Pharmacy, India, Int. J. Appl. Biol. Pharm., 5(3), 186–189.

Chudiwal A.K., Jain D.P. and Somani R.S., 2010, Alpinia galanga Willd.- An overview on Phyto-Pharmacological Properties, Indian J. Nat. Prod. Resour, 1(2), 143–149. Link

Elfahmi, et al, 2006, The Indonesian Tradisional Herbal Medicine, Link, Cited, November 25, 2016.

Hasima, N., Aun, L.I.L., Azmi, M.N., Aziz, A.N., Thirthagiri, E., Ibrahim, H., et al., 2010, 1S-1’-Acetoxyeugenol Acetate: A New Chemotherapeutic Natural Compound Against MCF-7 Human Breast Cancer Cells, Phytomedicine, 17(12), 935–939. CrossRef

KemenKesRI, 2016, Hilangkan mitos tentang kanker, 1–2.

KemenKesRI, 2015, Infodatin Pusat Data dan Informasi Kesehatan RI, Jakarta: Kementerian Kesehatan RI

Lee, C.C. and Houghton, P., 2005, Cytotoxicity of Plants from Malaysia and Thailand used Traditionally to Treat Cancer, J. Ethnopharmacology, 100, 237-243. CrossRef

Matsuda, H., Ando, S., Morikawa, T., Kataoka, S. and Yoshikawa, M., 2005. Structure–activity Relationships of 1′ S-1′-acetoxychavicol Acetate for Inhibitory Effect on NO Production in Lipopolysaccharide-activated Mouse Peritoneal Macrophages, Bioorg. Med. Chem. Lett., 15(7), 1949-1953. CrossRef

Rajabalian, S., Foroumadi, A., Shafiee, A. and Emami, S., 2007, Functionalized N-(2-oxyiminoethyl) Piperazinyl quinolonesas New Cytotoxic Agents, J. Pharm. Pharm. Sci., 10(2), 153-158. Link

Samarghandian, S., Hadjzadeh, M.-A.-R., Afshari, J. T. and Hosseini, M, 2014, Antiproliferative Activity and Induction of Apoptotic by Ethanolic Extract of Alpinia galanga rhizhome in Human Breast Carcinoma Cell Line, BMC Complement Altern. Med., 14, 192. CrossRef


Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:




Indonesian Society for Cancer Chemoprevention