Anti-Breast Cancer Potency of Multistage Extraction from Jamur Dewa (Agaricus blazei Murill) Solvents on MCF-7 Cells

Misgiati Misgiati, Sukardiman Sukardiman, Aty Widyawaruyanti


ABM (Agaricus blazei Murill) is a basidiomycetes fungus. ABM is used by people for the treatment of diabetes, antihypertention, anticholesterol, anticancer, and immunostimulant. ABM contains terpene, steroids, agaritine, vitamin C, vitamin E, and betaglucane. In this research, ABM extract was tested as an anti-breast cancer in vitro using MCF-7 breast cancer cells. The extract was obtained from the multistage extraction process of several solvents in turn, the solvent used, among others, n-hexane, dichloromethane (DCM), chloroform, ethyl acetate, butanol, and water. The results of the research were the obtained IC50 value from n-hexane extract 247,17 μg /ml; extract DCM 227μg/ml ; chloroform extract 215,64 μg /ml ; extract of ethyl acetate 234,9 μg/ml ; butanol extract 500,78 μg/ml; while the water extract was inactive. Based on these results can be considered for further research to fractionate in order to know which class compounds have the potency as anticancer within the extracts.

Key words : Agaricus blazei, multistage extraction, MCF-7 cells.

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Akiyama, H., Endo, M., Matsui, T., Katsuda, I., Emi, N., Kawamoto, Y., et al., 2011, Agaritine from Agaricus blazei Murrill Induces Apoptosis in the Leukemic Cell Line U937, Biochim. Biophys. Acta., 1810(5), 519-525. CrossRef

American cancer society, 2017, Breast cancer: key statistic of breast cancer. Available at:, accessed on January, 11th 2017. Link

Cho, S.M., Park, J.S., Kim, K.P., Cha, D.Y., Kim, H.M. and Yoo, I.D., 1999, Chemical Features and Purification of Immunostimulating Ppolysaccharides from the Fruit Bodies of Agaricus blazei, Kor. J. Mycol., 27(2), 170-174. Link

Desen, W., 2008, Buku Ajar Onkologi Klnik, 2nd Edition, Jakarta: Fakultas Kedokteran Universitas Indonesia.

Ekowati, H., Prasasti, E. and Rastuti, U., 2011, The Active Fraction from Nigella sativa and its Activity Against T47D Cell Line, Indones. J. Chem., 11(3), 217-222. CrossRef

Fujimiya, Y., Suzuki, Y., Oshiman, K.I., Kobori, H., Moriguchi, K., Nakashima, H., et al 1998, Selective Tumoricidal Effect of Soluble Proteoglucan Extracted from the Basidiomycete, Agaricus blazei Murill, Mediated Via Natural Killer Cell Activation and Apoptosis, Cancer Immunol. Immunother., 46(3), 147-159. CrossRef

Gao, L., Sun, Y., Chen, C., Xi, Y., Wang, J. and Wang, Z., 2007, Primary Mechanism of Apoptosis Induction in a Leukemia Cell Line by Fraction FA-2-b-ss Prepared from the Mushroom Agaricus blazei Murill, Braz. J. Med. Biol. Res., 40(11), 1545-1555. CrossRef

Hikichi, M., Hiroe, E. and Okubo, S., 1999, Protein Polysaccharide 0041. European Patent 0939082, 9 January 1999.

Hirotani, M., Masuda, M., Sukemori, A., Hirotani, S., Sato, N. and Yoshikawa, T, 2005, Agariblazeipirol C from the Cultured Mycelia of The Fungus, Agaricus blazei, and The Chemical Conversion of Blazeipirol A, Tetrahedron, 61(2005), 189-194. CrossRef

Huang, S.J. and Mau, J.L., 2006, Antioxidant Properties of Methanolic Extracts from Agaricus blazei with Various Doses of γ-Irradiation, Food Sci. Technol., 39(7), 707-716. CrossRef

Itoh, H., Ito, H. and Hibasami, H., 2008. Blazein of a New Steroid Isolated from Agaricus blazei Murrill (himematsutake) Induces Cell Death and Morphological Change Indicative of Apoptotic Chromatin Condensation in Human Lung Cancer LU99 and Stomach Cancer KATO III Cells, Oncol. Rep., 20(6), 1359-1361. Link

Kawagishi, H., Kanao, T., Inagaki, R., Mizuno, T., Shimura, K., Ito, H., Hagiwara, T. and Nakamura, T., 1990, Formolysis of a Potent Antitumor (1→ 6)-β-d-glucan-protein Complex from Agaricus blazei Fruiting Bodies and Antitumor Activity of the Resulting Products, Carbohydr. Polym., 12(4), 393-403. CrossRef

Kimura, Y., Kido, T., Takaku, T., Sumiyoshi, M. and Baba, K., 2004, Isolation of an Anti‐Angiogenic Substance from Agaricus blazei Murill: Its Antitumor and Antimetastatic Actions, Cancer Sci., 95(9), 758-764. CrossRef

Kusumastuti, R., 2013, Efek Ekstrak Kloroform Daun Tapak Dara (Catharanthus roseus [L.] G. Don) Var. Albus dan Roseus dalam induksi apoptosis berdasarkan ekspresi procaspase-3 pada sel HeLa, Dissertation, Universitas Gadjah Mada, Yogyakarta.

Machana, S., Weerapreeyakul, N., Barusrux, S., Nonpunya, A., Sripanidkulchai, B. and Thitimetharoch, T., 2011, Cytotoxic and Apoptotic Effects of Six Herbal Plants Against the Human Hepatocarcinoma (HepG2) Cell Line, Chin. Med., 6(1), 39, doi: 10.1186/1749-8546-6-39. CrossRef

Misgiati, M., 2011, Kemampuan Antikanker Ekstrak Jamur Dewa (Agaricus blazei Murill) Pada Sel kanker Serviks Sebagai Bahan Bacaan Pengobatan Tradisional, Thesis, Disertation and thesis graduate programme, Universitas Negri Malang, Malang.

Mizuno, T., Inagaki, R., Kanao, T., Hagiwara, T., Nakamura, T., Ito, H., et al.,1990, Antitumor Activity and Some Properties of Water-Insoluble Hetero-glycans from “Himematsutake,” the Fruiting Body of Agaricus blazei Murill, Agric. Biol. Chem., 54(11), 2897-2905. CrossRef

Nagaoka, M.H., Nagaoka, H., Kondo, K., Akiyama, H. Maitani, T., 2006, Measurement of a Genotoxic Hydrazine, Agaritine, and Its Derivatives by HPLC with Fluorescence Derivatization in the Agaricus Mushroom and Its Products, Chem. Pharm. Bull., 54(6), 922–924. CrossRef

Takaku, T., Kimura, Y. and Okuda, H., 2001, Isolation of an Antitumor Compound from Agaricus blazei Murill and Its Mechanism of Action, J. nutr., 131(5), 1409-1413. CrossRef

Winarno, E., 2011, Uji Sitotoksik Ekstrak Kapang Aspergillus Sp. terhadap Sel Kanker Payudara T47D, Thesis undergraduate programme, Universitas Indonesia, Jakarta.

Yu, C.H., Kan, S.F., Shu, C.H., Lu, T.J., Sun-Hwang, L. and Wang, P.S., 2009, Inhibitory Mechanisms of Agaricus blazei Murill on the Growth of Prostate Cancer In vitro and in vivo, J. Nutr. Biochem., 20(10), 753-764. CrossRef


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