Combination of Solanum nigrum L. Herb Ethanolic Extract and Doxorubicin Performs Synergism on T47D Breast Cancer Cells

Anindyajati Anindyajati, Sarmoko Sarmoko, Dyaningtyas Dewi Pamungkas Putri, Adam Hermawan, Edy Meiyanto


Leunca (Solanum nigrum L.) has been proven to possess anticancer activity on some type of cancer cells. In vitro study of solamargine found in the herb showed cytotoxic effect against several breast cancer cell lines, such as T47D and MDA-MB-31. Hence, further study on its potential as a co-chemotherapeutic agent needs to be conducted, in order to overcome resistance problem commonly found in cancer chemotherapy. This study aimed to examine the cytotoxic activity of leunca herb ethanolic extract (LEE) alone and its combination with doxorubicin. Single and combinational treatment of LEE and doxorubicin on T47D breast cancer cells were done, and their viability representing cytotoxicity were analyzed by using MTT assay to determine the IC50 value and combination index (CI) to evaluate the combinational effect. Twenty four hours-treatment of LEE alone gave cytotoxicity activity showing a dose-dependent manner with the IC50 of 47 µg/ml, while combinational treatment showed that 4 µg/ml LEE was found to be synergist with 4 nM doxorubicin on T47D cells, with the optimum CI value of 0.59. This result shows that Solanum nigrum L. is potential to be proposed as doxorubicin co-chemotherapeutic agent against breast cancer. Further study on its molecular mechanism needs to be conducted.

Key words: Solanum nigrum, doxorubicin, synergist, breast cancer

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Anonym, 2009, Rumah Sakit Kanker Dharmais’ Medical Report; Statistik Kanker: 10 Besar Kanker Tersering RSKD Rawat Jalan (Kasus Baru) tahun 2007, available in, accessed on March 2010.

Everist, S.L. 1974, Poisonous Plants of Australia, Angus and Robertson, Sydney.

Gibbs, J.B., 2000, Anticancer Drug Targets: Growth Factor and Growth Factor Signaling, J. Clin. Inves., 105 (1): 9-13.

Hu, K., Kobayashi H., Dong A., Jing Y., Iwasaki S., and Yao X., 1999, Antineoplastic Agents III: Steroidal Glycosides from Solanum nigrum, Planta Med., 65 (1): 35-38.

Ji, Y.B., Gao S.Y., Ji C.F., and Zou X., 2008, Induction of Apoptosis in HepG2 Cells by Solanine and Bcl-2 protein, J. Ethnopharmacol., 115 (2): 194-202.

Junedi, Sendy, 2010, Peningkatan Aktivitas Sitotoksik Doxorubicin oleh Naringenin pada Sel Kanker Payudara T47D melalui Modulasi Siklus Sel dan Pemacuan Apoptosis, Thesis, Program Pascasarjana Program Studi Ilmu Farmasi Universitas Gadjah Mada, Yogyakarta.

Liang, C.H., Shiu L.Y., Chang L.C., Sheu H.M., Tsai E.M., and Kuo K.W., 2008, Solamargine Enhances HER2 Expression and Increases the Susceptibility of Human Lung Cancer H661 and H69 Cells to Trastuzumab and Epirubicin, Chem Res. Toxicol., 21 (2): 393-399.

Liu, L.F., Liang C.H., Shiu L.Y., Lin W.L., Lin C.C., and Kuo K.W., 2004, Action of Solamargine on Human Lung Cancer Cells-Enhancement of The Susceptibility of Cancer Cells to TNFs, FEBS Lett., 577 (1-2): 67-74.

Mechetner, E., Kyshtoobayeva A., Zonis S., Kim H., Stroup R., Garcia R., Parker R.J., and Fruehauf, J.P., 1998, Levels of Multidrug Resistance (MDR1) P-Glycoprotein Expression by Human Breast Cancer Correlate with in vitro Resistance to Taxol and Doxorubicin, Clin. Cancer Res., 4, 389-398.

Pandanilam, B.J., 2003, Cell Death Induced by Acute Renal Injury: A Perspective on the Contributions of Apoptosis and Necrosis, Am. J. Physiol. Renal Physiol., 284: 608-627.

Reynolds, C.P. and Maurer B.J., 2005, Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models, Methods in Molecular Medicine, 110, 173-183.

Saijo, R., Murakami K., Nohara T., Tomimatsu T., Sato A., and Matsuoka K., 1982, Studies on the Constituents of Solanum Plants II: Constituents of the Immature Fruits of Solanum nigrum, Yakugaku Zasshi, 102: 300-305.

Wattanapitayakul, S.K., Chularojmontri L., Herunsalee A., Charuchongkolwongse S., Niumsakul S., and Bauer J.A., 2005, Screening of Antioxidants from Medicinal Plants for Cardioprotective Effect against Doxorubicin Toxicity, Basic and Clin. Pharmacol. and Toxicol., 96 (1): 80-87.

Weller, R.F. and R.H. Phipps., 1979, A Review of The Black Nightshade (Solanum nigrum L.), Protection Ecology, 1: 121-139.


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