Cinnamomum Essential Oil Prevents DNA Damage-Induced by Doxorubicin on CHO-K1 Cells

Layung Sekar Sih Wikanthi, Nindi Wulandari, Yuni Fajar Esti, Nur Fitra Sari, Ratna Asmah Susidarti

Abstract


DNA damage usually happens due to the several chemical materials that induce genotoxic effect in normal cells. Cinnamon essential oil (CEO), which contains cinnamaldehyde as its major compound, has been reported to possess antioxidant activity to prevent DNA damage. The aim of this study is to evaluate the genotoxic and cytotoxic effect of CEO on doxorubicin-induced Chinese Hamster Ovary (CHO-K1) cells. The cytotoxic effect of CEO was determined by MTT assay with the parameter of IC50 while the genotoxic effect was carried out by micronucleus (MN) assay by using acridine orange fluorescent staining with the parameter of MN/1000 cells reduction number. Based on MTT assay, CEO showed cytotoxic activity with the IC50 value of 30 μg/ml and for MN assay, 3 μg/ml (1/10 IC50) of CEO decreased the percentage of micronucleus per 1000 cells up to 94,55%. Thus, the result can be summarized that CEO does not induce genotoxic and has the potency to prevent DNA damage caused by doxorubicin on CHO-K1 cells.

Keywords: genotoxic, cinnamomum essential oil (CEO), micronuclei assay, in vitro


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References


Al-Dhubiab, B.E., 2012, Pharmaceutical Applications and Phytochemical Profile of Cinnamomum burmannii, Pharmacog. Rev., 6(12), 125-131. CrossRef

Bonassi, S., Coskun, E., Ceppi, M., Lando, C., Bolognesi, C., Burgaz, S., et al., 2011, The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL): the role of life-style, host factors, occupational exposures, health status, and assay protocol, Mutat. Res., 728(3), 88–97. CrossRef

Choi, E.M. and Hwang, J.K., 2005, Screening of Indonesian Medicinal Plants for Inhibitor Activity on Nitric Oxide Production of RAW264.7 cells and antioxidant activity, Fitoterapia, 76(2), 194–203. CrossRef

Guengerich, FP., 1992, Metabolic Activation of Carcinogens, Pharmacol. Ther., 54(1), 17-61. CrossRef

Shah, S.U., 2012, Importance of Genotoxicity & S2A guidelines for genotoxicity testing for pharmaceuticals, IOSR J. Pharm. Biol. Sci., 1(2), 2278-3008. CrossRef

Shi, M., Xu, B., Azakami, M., Morikawa, T., Watanabe, K., Morimoto, K., et al., 2005, Dual Role of Vitamin C in An Oxygen-sensitive System: Discrepancy between DNA Damage and Cell Death, Free Radic. Res., 39(2), 213-220. CrossRef

Zhou, B.B. and Elledge, S.J., 2000, The DNA Damage Response: Putting Checkpoints in Perspective, Nature, 408(6811), 433–439. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev8iss1pp27-31

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