Phaleria macrocarpa Fruit Extract Inhibits NF-ĸB Activation and Apoptosis Induction on HeLa Cells

Meirizky Zulharini S., Amalia Miranda, Lina Permatasari, Hilyatul Fadliyah, Riris Istighfari Jenie


NF-κB is a transcription factor and if activated, it induces apoptosis inhibition. Phalerin from Phaleria macrocarpa fruits expected to inhibit NF-κB activation. This research is to investigate anticancer mechanism of Phaleria macrocarpa fruits extracts (EBMD) in NF-κB pathway. Molecular docking assay was performed to determine phalerin affinity to IKK. Cytotoxic activity was observed by MTT assay. Double staining was performed to determine the apoptotic cells. Docking score of phalerin to IKK is -60. The IC50 value of EBMD is 629 μg/mL. Apoptosis profile shows (shown that) many cells undergoing apoptosis after treatment. Thus, EBMD potentially inhibits activation of NF-κB pathway and triggers apoptosis on HeLa cells.

Keywords : NF-κB, Phaleria macrocarpa, sel HeLa, Bcl-2, IKK, molecular docking

Full Text:



Agung, B.S., Faried, A., Arifin, M.Z., Wiriadisastra, K. and Ohta, T., 2008, Herbal Medicine Isolation, Phaleria macrocarpa for Primary Glioblastoma Multiforme Cells, Ann. Epidemiol, 18(9), 718. CrossRef

Anonymous, 2012, Yayasan Kanker Indonesia-Jakarta Race,, Cited July 14, 2014.

Backer, C. and van den Brink, R., 1965, Flora of Java (Spermatophytes Only), Volume II, Groningen: Noordhoff.

Baldwin, A.S., Jr., 2001, The Transcription Factor NF-kB and Human Disease, J. Clin. Investig., 107(1), 3–6. CrossRef

Ghosh, S. and Hayden, M.S., 2008, New Regulators of NF-κB in Inflammation, Nat. Rev. Immunol., 8(11),837-848. CrossRef

Giardina, C. and Hubbard, A.K., 2002, Growing Old With NF-kappaB, Cell Stress Chaperones, 7(2), 207-212.

Gilmore T.D., 2006, Introduction to NF-B: players, pathways, perspectives, Oncogene, 25(51), 6680–6684. CrossRef

Hou, S. Y., Wu, S. and Chiang, C., 2002, Transcriptional Activitty among High an Low Risk human Papillomavirus proteins E2 Proteins correlates of E2 DNA binding, J. Biol. Chemy., 227(47), 45619-29. CrossRef

Kim, H., Kim H., Ashik, M., Rajendra, G., Kwang, S., Somi, K., 2012, Induction of Apoptosis by Ethanolic Extracts of Mango Peel and Comparative Analysis of the Chemical Consists of Mango Peel and Flesh, Food Chem., 133(2), 416-422. CrossRef

Lodish, H., Berk, A. and Zipursky, S.L., 2000, Molecular Cell Biology, 4th edition, New York: W. H. Freeman.

Li, R., Cheng, Y. and Gao, C., 2006, Enhancing Radiosensitivity of Hela Cells by Combining Transfection of bcl-2 c-myc ASODNs, Bull. Cancer, 93(11), E119-25. Link

Taraphdar, A.K., Roy, M. and Bhattacharya R.K., 2001, Natural Products as Inducers of Apoptosis: Implication for Cancer Therapy and Prevention, Curr. Sci., 80(11), 1387-1396. Link

Thu, Y.M. and Richmond, A., 2010, NF-κB Inducing Kinase: A Key Regulator in the Immune System and Cancer, Cytokine Growth Factor Rev., 21(4), 213-226.

Timothy, S., Canavan, M.D., Nipa, R. and Doshi, M.D., 2000, Cervical Cancer, Cited December 25, 2011.

Verma, I.M., 2004, Nuclear Factor (NF)-kappa B Proteins: Therapeutic Targets, Ann. Rheum. Dis., 62(2), 1157-1161. CrossRef

Voorhees, P.M., Dees, E.C., O’Neil, B. and Orlowski, R.Z., 2003, The proteasome as a target for cancer therapy, Clin. Cancer Res., 9(17), 6316-25. Link

WHO/ICO, 2010, Information Centre on HPV and Cervical Cancer. Human Papilloma Virus and Cancers in World,, Cited December 20, 2012.


Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention

Indexed by:





Indonesian Society for Cancer Chemoprevention