Immunostimulant Effect of Garlic Chives Leaf Ethanolic Extract (Allium tuberosum) by Increasing Level of Antioxidant at Rats Doxorubicin-Induced Rats

Ika Rahmawati Sutejo, Kiky Martha Ariesaka, Fuad Adi Prasetyo, Mudzakkir Taufiqurrqhman, Ain Yuanita Insani, Brilliant Givya Ariansari

Abstract


Cancer is one of the leading causes of death in the world, approximately 14 million new cases and 8.2 million deaths each year. Doxorubicin is a well-known chemotherapy drug which frequently used in treating various types of cancer. However,  doxorubicin posesses several side effects including cardiotoxic, hepatotoxic, nephrotoxic, and immunosuppression. One of the natural product that can be used as an adjuvant of doxorubicin to reduce the toxic effects is garlic chives (Allium tuberosum). The purpose of this study was to determine the effect of Allium tuberosum based on hematological profile, levels of CD4+, CD8+, and MDA serum of male Wistar rats which induced by doxorubicin. The hematological profile was analyzes by blood smear, levels of CD4+ and CD8+ were conducted by flowcytometry and levels of MDA serum were determined by spectrofotometry. The results showed that the etanolic extract of Allium tuberosum (EAT) increased neutrophil and lymphocyte, percentage of CD4+ cells (p<0.01) and CD8+ cells. It also decreased the levels of serum MDA (p<0.01). These results indicated that EAT work as immunostimulant possibly through an antioxidant mechanism (MDA). It can be concluded that EAT can be developed as adjuvant for doxorubicin.

Keywords: doxorubicin, Allium tuberosum, immunostimulant, antioxidant, CD4+, CD8+ 


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References


Alessandra, L., Stefano, F., Gaetano, B.and Salvatore S., 2007., Long-term Follow-Up of Patients with Doxorubicin-induced Cardiac Toxicity After Chemotherapy for Osteosarcoma, Anti Cancer Drugs, 18(6), 737-744. CrossRef

Chaudhary, D., Khatiwada, S., Sah, S.K.,Tamang M.K., Bhattacharya S. and Jha C.B., 2016, Effect of Doxorubicin on Histomorphology of Liver of Wistar Albino Rats, J. Pharm. Pharmacol., 4(2016), 186-190. CrossRef

Chirumbolo, S., 2010, The Role of Quercetin, Flavonols and Flavones in Modulating Inflammatory Cell Function, Inflamm. Allergy Drug Targets, 9(4), 263-85. Link

Feng, Y., Zhu, X., Wang, Q., Jiang, Y., Shang, H., Cu, L., et al., 2012, Allicin Enhances Host Pro-inflammatory Immune Responses and Protects Against Acute murine malaria infection, Malar.

J., 11(1), 268-276. CrossRef

Gill, L., Martinez, G., Gonzalez, I., Tarinas,A., Alvarez, A., Giuliani, A., et al.,2003. Contribution to Characterization of Oxidative Stress in HIV/AIDS Patients, Pharmacol. Res., 47(3), 217-224. Link

Kasianningsih, S., Rivanti, E., Pratama, R.H.,Pratama, N.R., Ikawati, M. and Meiyanto, E., 2011., Taraxacum officinale Leaves Etanolikic Extract as Immunostimulatory Agent for Reducing Side Effect of

Doxorubicin in Sprague Dawley Rats, Indones. J. of Cancer Chemoprevent., 2(1), 135-140. Link

Kementrian Kesehatan RI, 2015, SituasiPenyakit Kanker, Jakarta: Pusatdata dan informasi Kementrian Kesehatan RI.

Li. M., Tang, Z., Lv, S., Song, W., Hong, H.,Jing, X., et al., 2014, Cisplatin Crosslinked pH Sensitive Nanoparticles for Efficient Deliveryof Doxorubicin, Biomaterials, 35(12),3851-3864. CrossRef

Mohebbati, R., Abbsnezhad, A., Khajavi,R.A., Mousavi, S.M. and Haghshenas M., 2016, Effect of HydroalcholicExtract of Nigella sativa on Doxorubicin-Induced Functional Damage of Kidney in Rats, Horizon Med. Sci., 22(1), 13-20. CrossRef

Roomi, W., Kalinovsky, T., Roomi, N.W.,Rath, W. and Niedzwiecki, A., 2014, Prevention of Adriamycin-induced Hepatic and Renal Toxicity in Male BALB/c Mice by a Nutrient Mixture, Exp. Ther. Med., 7(4),1040–1044. CrossRef

Song, W.Y. and Choi J.H., 2016, Effects of Oenanthe javanica and Allium tuberosum on Lipid Content in Rats Fed a High-fat High-cholesterol Diet, J. Life Sci., 26(3), 302-308. CrossRef

Tacar, O., Sriamornsak, P. and Dass, C.R.,2012, Doxorubicin: An Update onAnticancer Molecular Action, Toxicityand Novel Drug Delivery Systems, J. Pharm. Pharmacol., 65(2), 157-170. CrossRef

World Health Organization, 2015, Cancer, http://www.who.int/mediacentre/factsh

eets/fs297/en/, Cited June 8, 2016.

Zhang, F., Zhang, X., Wang, B.C. and Wang,F., 2009, Pachymic Acid, A Novel Compound for Anti-Rejection: Effect in Rats Following Cardiac Allograft Transplantation, Chin. Med. J., 122(23), 2898-2902. Link

Zhang, X., Li, W., Wu, Y., Zheng, T., Li, W.,Qu, S., et al., 2005b, Proanthocyanidin from Grape Seeds Potentiates Anti-tumor Activity of Doxorubicin via Immunomodulatory Mechanism, Int. Immunopharmacol., 5(7-8), 1247-1257. CrossRef

Zhang, X.Y., Li, W.G., Wu, Y.J. and Gao, M.T., 2005a, Amelioration of Doxorubicin-Induced Myocardial Oxidative Stress and Immuosuppression by Grape Seed Proanthocyanidins in Tumour-bearing Mice, J. Pharm. Pharmacol., 57(8), 1043-1051. CrossRef




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev7iss3pp93-98

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