The Effect of Marigold Leaves and Doxorubicin Toward Cell Cycle and Apoptosis of T47D Cells

Anis Fauzia, Umi Any Tiyas Wati, Cahyaning Gita Rizkita, Eka Wahyuni Nurul Qori'ah, Ibrahim Arifin


Doxorubicin is a commonly used chemotherapeutic agent in patients with breast cancer. The risk of side effect using of doxorubicin  such as cardiomyopathy and congestive heart failure which can lead to death. One of the  approach to overcome overloaded use of doxorubicin is the combined use with a chemopreventive agent (cochemotherapy), including the leaf extract of marigolds (Cosmos caudatus Kunth.). This research aims to reviewing the effect of the methanol extract of leaves of marigolds on the cytotoxic activity of doxorubicin in modulating cell cycle and apoptosis of breast cancer cells T47D. Determination of the cytotoxic activity of methanol extract of leaves of marigolds and doxorubicin as well as a combination of both conducted by MTT assay. modulation surveillance of cell cycle and apoptosis induction is done by using flowcytometry and analyzed by FACS Calibur program. Cytotoxicity assay single treatment of the methanol extract of leaves of marigolds produce use values of IC50 504,840 μg/ml, whereas IC50 values doxorubicin is 141,055 nM. The synergistic effect was shown a combination of methanol extract leaves marigolds and doxorubicin at concentrations of 84,17 μg/ml -23,5 nM; 84,17 μg/ml -47 nM; 126,25 μg/ml -23,5 nM; 252,5 μg/ml -35,25 nM and 252,5 μg/ml -71 nM with a combination index value (CI) consecutively of 0,5; 0,6; 0,6; 0,6 and 0,6. Observations modulation of cell cycle and apoptosis induction combination of methanol extract leaves marigolds and doxorubicin at concentrations of 84,17 μg/ml -23,5 nM, said that a combination of the methanol extract of leaves of marigolds and doxorubicin to inhibit the proliferation of cells in G2 / M phase and able to induce apoptosis of breast cancer cells T47D.

Keywords: methanol extract of leaves of marigolds, doxorubicin, flowcytometry, cell cycle, apoptosis.

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Abas F., Shaari, K., Lajis, N.H., Israf, D.A. and Kalsom, Y.U., 2003, Anti oxidative and Radical Scavenging Properties of The Constituents Isolated From Tagetes patula Jusst, Nat. Prod. Science, 9(4), 245-248. Link

Ayers, L., Kohler, M., Harrison, P., Sargent, I., Dragovic, R. and Schaap, M., 2011, Measurement of Circulating Cell-derived Microparticles by Flowcytometry: Source of Variability within the Assay, Thromb. Res., 127(4), 370-377. CrossRef

Depkes RI, 1979, Farmakope Indonesia, 3rd Edition, Jakarta: Departemen Kesehatan Republik Indonesia.

Drummond, C., 2007, The Mechanism of Anti-tumour Activity of the DNA Binding Agent SN 28049, Thesis, New Zealand: University of Auckland.

Fita, F.E., Listianingsih, D., Pradana, R.G. and Safitri E.I., 2015, Kolaborasi Ekstrak Metanol Daun Kenikir dan Doksorubisin sebagai Upaya Pengembangan Agen Ko-kemoterapi Terhadap Sel Kanker Payudara T47D, Studi in Vitro dan in Silico, Semarang: Seminar Nasional Peluang Herbal Sebagai Alternative Medicine.

Gattenlohner, S., Etschmann, B., Kunzmann, V., Thalheimer, A., Hack, M. and Kleber, G., 2009, Concordance of KRAS/BRAF Mutation Status in Metastatic Colorectal Cancer Before and After Anti-EGFR Therapy, J. Oncol., 2009(2009), 1-8. CrossRef

Gibellini, L., Marcello P., Milena N., Jonas P.M., Sara, D.B., Erika, R., et al., 2011, Quercetin and Cancer Chemoprevention, Evid. Based Complementary Altern. Med., 2011, 1-15. CrossRef

IARC, 2013, Latest World Cancer Statistics Global Cancer Burden Rises To 14.1 Million New Cases In 2012: Marked Increase In Breast Cancers Must Be Addressed, Geneva: World Health Organization.

Jemal, A., Siegel, R., Ward, E., Murray, T., Xu, J.T. and Michael J.T., 2007, Cancer Statistic 2007, CA Cancer J. Clin., 57(1), 43-66. Link

Mossmann, T., 1983, Rapid Colorimetric Assay for Cellular Growth and Survival, Application to Proliferation and Cytotoxicity Assays, J. Immunol. Methods, 65(1-2), 55-63. Link

Rock, E. and DeMichele, A., 2003, Nutritional Approaches to Late Toxicities of Adjuvant Chemotherapy 86 in Breast Cancer Survivors, American Society for Nutritional Sciences, J. Nutr., 133(11 Suppl 1), 3785S-3793S. Link

Singal, P.K., Iliskovic, N., Li, T. and Kaur, K., 2001, Heart Failure Due to Doxorubicin, Kuwait Medical Journal, 33(2), 111-115.

Smith, L., Watson, M.B., O’Kane, S.L.,Drew, P.J. and Lind, M.J., 2006. The Analysis of Doxorubicin Resistance in Human Breast Cancer Cell Using Antibody Microarray, Mol. Cancer Ther., 5(8), 2115-2120. CrossRef


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