Effect of Basil Leaves (Ocimum sanctum L.) Infusion as Hepatoprotective Agent Induced by Paracetamol

Ni Nyoman Yuliani, Jefrin Sambara, Maria Hilaria, Harlinda Harlinda


Indonesia has  biodiversity potential to be developed as medicinal plants, such as basil leaves (Ocimum sanctum L.). Basil was reported to have a very high antioxidant activity in vitro. The aim of this study was to determine the effect of basil leaves (Ocimum sanctum L.) infusion to liver based Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) value in each dose. 18 rats were divided into 6 groups.,  control group,  negative control group treated with CMC Na 0.5%,  positive control group treated with Curcuma 3.6 mg / 200gBB, groups of 4-6 consecutive given a 80 mg  dose infusion basil / 200 gBB, 160 mg / 200 gBB, 320 mg / 200 gBB for 8 consecutive days, on the day of the 4th and 8th all treatment groups induced by toxic doses of paracetamol (500 mg / 200 gBB) except the normal control group 1, The research data in the form of enzyme activity of AST and ALT were analyzed using parametric and nonparametric ANOVA, and Friedman test with the level of trust  then followed by SNK test and Bnj test. The statistical test result with a 95% of level of trust that shown basil infuse with a dose of 80mg / 200gBB, 160mg / 200gBB, 320mg / 200gBB have hepatoprotective effects in rats induced by paracetamol 500mg / 200gBB. Based on the result of changes in average levels of AST on the fourth day and the eighth day of the three treatment infusion, infusion at a dose of 160 mg / 200gBB most effectively reduce average levels of AST and a group that has the average AST closest to the control group is positive, but infusion at a dose of 320mg / 200gBB the group that has the closest average ALT positive control group.

Keywords : hepatoprotective, Ocimum sanctum L, Paracetamol

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Anief, M., 2000, Science Theory and Practice of medicine dispensing, Yogyakarta: Gadjah Mada University Press.

Anonymous, Benefits of Basil, http://wwwsmallcrab.com/kesehatan/216-manfaat-kemangi, Cited July 12, 2015.

Anonymous, Hepatoprotective Herbs, http://buletinkesehatan.com/hepatoprotektordari-tanaman-herbal-untuk-mengobati-penyakit-hati/, Cited December 1, 2014.

Anonymous, 2005, Pharmaceutical, Yogyakarta: Gadjah Mada University Press.

Anonymous, 2003, Pharmacology and Therapy, 4th edition, Jakarta: Univeritas Indonesia Press.

Anonymous, 2013, Practical guide Pharmacology, Kupang: Health Polytechnic.

Anonymous, 1993, Screening of Pharmacology, Phytochemicals and Testing. Clinic. The Development of Natural Medicine, Jakarta: ____.

Biomed, C.M, and Lestari, 2011, Guidelines Basic Techniques for Health Laboratory, Jakarta: EGC Publishers.

Dahlan, M.S., 2009, Statistics for Medicine and Health. Salemba Medika. Jakarta Factors Affecting Enzymes Work, http://www.jendelasarjana.com/2013/09/faktor-yang-mempengaruhi-kerja-enzim, Cited July 12, 2015.

Gandjar, I.G. and Rohman, A., 2007. _____, Yogyakarta: Student Library.

Gunawan, D. and Mulyani, S., 2004, Volume I. Pharmacognosy Sower Organization, Jakarta: ____.

Harborne, J.B., 1987, Metode Fitokomia, Bandung: Bandung Institute of Technology Press.

Iklas, N., 2013, Antioxidant Activity Test Extract Herb Basil (Ocimum americanum Linn) Method DPPH (2,2-Diphenyl-1-picrylhydrazyl), Essay, Medicine Faculty of Pharmacy and Health Sciences, Jakarta.

Kamajaya, 2006, Physics, Jakarta: Grafindo.

Katzung, B.G., 2002, Basic and Clinical Pharmacology, 8th Edition, Jakarta: Salemba Medika.

Kee, J.L., 2007, Examination and Diagnostic Guidelines, Jakarta: EGC.

Kementrian Kesehatan RI, 2003, Decree of the Minister of Health of the Republic of Indonesia No. 1076 of 2003 on the Implementation of Traditional Medicine, Jakarta: Menteri Kesehatan Republic of Indonesia.

Kementrian Kesehatan RI, 2011, Guidelines for Clinical Data Interpretation Jakarta: Menteri Kesehatan Republic of Indonesia.

Kementrian Kesehatan RI, 2001, Inventory of Medicinal Plants Indonesia, Volume II, Jakarta: Menteri Kesehatan Republic of Indonesia.

Kementrian Kesehatan RI, 1995, Materia Medika Indonesia Volume VI, Jakarta: Menteri Kesehatan Republic of Indonesia.

Kementrian Kesehatan RI, 2010, Regulation of the Minister of Health of the Republic of Indonesia Number 003 of 2010 on Saintifikasi Medicinal Research-Based Health Services, Jakarta: Menteri Kesehatan Republic of Indonesia.

Moelyono, M.W., 2007, Curcuma, Icon Herbal Medicine Indonesia, http://blogs.unpad.ac.id/moelyono/?=14.htm, Cited December 1, 2014.

Palupi, H.D., 2011, Effect of Ethanolic Extract of Leaves of Basil (Ocimum Sanctum L.) to Change Levels of Enzyme ALT, AST and Histology Hearts on White Male Wistar Rats were Induced Paracetamol, Essay, Universitas Setia Budi Surakarta, Surakarta.

Santoso, G., 2005., Quantitative and Qualitative Research Methodology, Jakarta: Perestasi Reader Publisher.

Sastroamidjojo, S., 2001, Original Drug Indonesia, Jakarta: Dian Rakyat.

Situmorang, T.E, 2010., Effect of Juice Papaya (Carica papaya L.) As hepatoprotective Against Liver Rats exposed Paracetamol, Essay, Universitas Sebelas Maret, Surakarta.

Suharman, and Muhammad, M., 1995., Instrumental Analysis, Surabaya: Airlangga University Press.

Tamboyang, J., 2001, Anatomy and Physiology for Nursing, Jakarta: EGC Publishers.

Tjay, T.H and Rahadja, K., 2007, Essential Drugs Issue VI, Jakarta: PT Elex Media Komputindo.

Watson, R., 2002, Anatomy and Physiology for Nursing, 10th Edition, Jakarta: EGC Publishers.

DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev7iss2pp68-73

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