Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX Cells

Ni Putu Linda Laksmiani, Ratna Asmah Susidarti, Edy Meiyanto

Abstract


Brazilein is a compound obtained in a large amount from the dried heartwood of Secang (Caesalpinia sappan L.). Brazilein has strong cytotoxic effect in several cancer cell lines. This research was designed to evaluate the cytotoxic effect of brazilein and its combination with a chemotherapy agent, doxorubicin on MCF-7/DOX breast cancer cells. In the cytotoxicity assay, MCF-7/DOX cells were cultured in the presence of brazilein solely and in combination with doxorubicin for 24 hours and cell viability was evaluated by using MTT assay. MTT assay showed a dose-dependent inhibition of cell proliferation with IC50 value of 37 µM. Brazilein increased doxorubicin’s cytotoxic activity on MCF-7/DOX cells. Both of single treatment with different concentration of brazilein 12.5 and 25 mM or doxorubicin 0.8 and 1 mM gave cell viability percentage above 80%, but combination of them led to decrease the cell viability percentage significantly. Based on this research, it can be concluded that brazilein is potential to be developed as a co-chemotherapy agent on breast cancer cell that have been resistant to doxorubicin. Futher study must be held to evaluate its molecular mechanism.

Keywords : brazilein, doxorubicin, MCF-7/DOX, cytotoxic.

 


Full Text:

PDF

References


Altieri, D.C., 2003, Validating Survivin as a Cancer Therapeutic Target, Nat Rev Cancer , 3, 46–54.

American Cancer Society (ACS), 2011, Breast Cancer Facts & Figures 2011-2012, Atlanta: American Cancer Society, Inc

Fortugno, P., Wall, N.R., and Giodini, A., 2002, Survivin Exists in Immunochemically Distinct Subcellular Pools and is Involved in Spindle Microtubule Function, J Cell Sci., 115, 85-575.

Gilmore, T. D., 2006, Introduction to NFkB: players, pathways, perspectives, Oncogene, 25, 6680-6684.

Lim, D.K., Choi, U., and Shin, D.H., 1997, Antioxidative Activity of Some Solvent Extract from Caesalpinia sappan Linn, Korean J. Food Sci. Technol. 28(1), 77−82.

Siddiqa, A., Long, L. M., Li, L., Marciniak, R.A., and Kazhdan, I., 2008, Expression of HER-2 in MCF-7 Breast Cancer Cells Modulates Anti-Apoptotic Proteins Survivin and Bcl-2 Via The Extracellular Signal-Related Kinase (ERK) and Phosphoinositide-3 Kinase (PI3K) Signaling Pathways, BMC Cancer 2008, 8, 129.

Tao, L.Y., Li, J.Y. and Zhang, J.Y., 2011, Brazilein Induced Cells Apoptosis in Human Breast Cancer MCF-7 and Its Mechanism, Journal of Sun Yat-Sen University (Medical Sciences), 32, 449-453.

Teng, W.Y., Yu, L.H., Chien, C.S., and Ray, L.H., 2005, Cytotoxic Acridone Alkaloids from Te Stem Bark of Citrus Maxima, Journal of The Chinese Chemical Society, 52, 1253-1255.

Yen, C., Goto, K.N., Hwang, T.S., Wu, P.C., Natschke, S.L.M., Lai, W.C., Bastow, K.F., Chang, F.B., Wu, Y.C., and Lee, K.H., 2010, Total Synthesis and Evaluation of Brazilein and Analogs as Anti-inflammatory and Cytotoxic Agents, Bioorg Med Chem Lett, 20(3), 1037–1039.

Zhong, B., Wu, Y.J., Pan, S., and Zheng, 2009, Brazilein Inhibits Survivin Protein and mRNA Expression and Induces Apoptosis in Hepatocellular Carcinoma HepG2 Cells, Neoplasma, 56 (5), 387-92.




DOI: http://dx.doi.org/10.14499/indonesianjcanchemoprev8iss2pp51

Refbacks

  • There are currently no refbacks.